了从玉米秸秆中获得高浓度可发酵糖以实现琥珀酸的高产，首先比较了蒸汽爆破和稀酸预处理2种方式对玉米秸秆酶解效果的影响，发现预处理后的玉米秸秆中含有较多影响琥珀酸发酵的抑制物，因此采用水洗及D301树脂共同脱毒以减少玉米秸秆水解液中的抑制物。研究结果表明，基于蒸汽爆破预处理的玉米秸秆酶解后总糖质量浓度最终能够达到147.24 g/L，高于稀酸预处理的115.61 g/L；经过蒸汽爆破、水洗脱毒后的玉米秸秆水解液初糖质量浓度为60 g/L时，蒸汽爆破组的琥珀酸的产量为25.32 g/L，得率为42.19%，比稀酸预处理组的琥珀酸产量提高11.59%。

Aim to obtain high titer fermentable sugars from corn stover for future high titer succinic acid production, the effect of steam explosion and dilute acid pretreatment methods on enzymatic hydrolysis were compared in this study at first.Many inhibitors were generated in the pretreatment process and could inhibit the fermentation of succinic acid.Water washing and resin (D301) adsorption methods were used to remove the inhibitors of corn stover hydrolysate.The results indicated that the total sugar concentration in steam explosion pretreated corn stover hydrolysate finally reached 147.24 g/L, which was higher than that of 115.61 g/L in dilute acid pretreated corn stover hydrolysate.25.32 g/L of succinic acid with 42.19% of yield was obtained from detoxified corn stover by only water washing after steam explosion pretreatment，it was 11.59% higher than dilute acid pretreatment group.This study provided the primary succinic acid production from lignocellulosic feedstock with potential of industrial applications, and suggested that the lignocellulose could be an important alternative feedstock used for future industrial succinic acid production.