稀酸和蒸汽爆破预处理玉米秸秆对琥珀酸发酵的影响

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  • 1(河南农业大学 生命科学学院,农业部农业微生物酶工程重点实验室,河南 郑州,450002) 2(郑州市质量技术监督检验测试中心,河南 郑州,450006)

网络出版日期: 2018-07-04

基金资助

河南省高校创新人才工程基金(15IRTSTHN014); 河南省高校创新人才工程基金(164100510016)

Effect of dilute acid and steam explosion pretreatment of corn straw on theproduction of succinic acid

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  • 1(College of Life Science, Henan Agricultural University, Key Laboratory of Agricultural Microbial Enzyme Engineering of the Ministry of Agriculture, Zhengzhou 450002, China) 2(Zhengzhou Testing Center of Quality Inspection and Technical Research, Zhengzhou 450002, China)

Online published: 2018-07-04

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摘要

了从玉米秸秆中获得高浓度可发酵糖以实现琥珀酸的高产,首先比较了蒸汽爆破和稀酸预处理2种方式对玉米秸秆酶解效果的影响,发现预处理后的玉米秸秆中含有较多影响琥珀酸发酵的抑制物,因此采用水洗及D301树脂共同脱毒以减少玉米秸秆水解液中的抑制物。研究结果表明,基于蒸汽爆破预处理的玉米秸秆酶解后总糖质量浓度最终能够达到147.24 g/L,高于稀酸预处理的115.61 g/L;经过蒸汽爆破、水洗脱毒后的玉米秸秆水解液初糖质量浓度为60 g/L时,蒸汽爆破组的琥珀酸的产量为25.32 g/L,得率为42.19%,比稀酸预处理组的琥珀酸产量提高11.59%。

本文引用格式

谢慧, 张东, 张兆昆, 等 . 稀酸和蒸汽爆破预处理玉米秸秆对琥珀酸发酵的影响[J]. 食品与发酵工业, 2018 , 44(5) : 57 -62 . DOI: 10.13995/j.cnki.11-1802/ts.016401

Abstract

Aim to obtain high titer fermentable sugars from corn stover for future high titer succinic acid production, the effect of steam explosion and dilute acid pretreatment methods on enzymatic hydrolysis were compared in this study at first.Many inhibitors were generated in the pretreatment process and could inhibit the fermentation of succinic acid.Water washing and resin (D301) adsorption methods were used to remove the inhibitors of corn stover hydrolysate.The results indicated that the total sugar concentration in steam explosion pretreated corn stover hydrolysate finally reached 147.24 g/L, which was higher than that of 115.61 g/L in dilute acid pretreated corn stover hydrolysate.25.32 g/L of succinic acid with 42.19% of yield was obtained from detoxified corn stover by only water washing after steam explosion pretreatment,it was 11.59% higher than dilute acid pretreatment group.This study provided the primary succinic acid production from lignocellulosic feedstock with potential of industrial applications, and suggested that the lignocellulose could be an important alternative feedstock used for future industrial succinic acid production.
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