不同食物来源乳酸细菌的安全性评价

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  • 1 (青岛科技大学 海洋科学与生物工程学院,山东 青岛, 266042) 2 (山东省农作物种质资源中心,山东 济南, 25000) 3 (青岛大学 附属医院呼吸科,山东 青岛, 266003)

网络出版日期: 2018-07-04

基金资助

国家绿肥产业技术体系(China Agriculture Research System-Green Manure, CARS-22);国家十二五科技支撑计划项目(2011BAD17B04);山东省重点研发计划(2017CXGC0311)

Safety assessment of lactic acid bacteria from different food products

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  • 1 (College of Marine Science and Bioengineering, Qingdao University of Science and Technology, Qingdao 266042, China) 2 (Shandong Center of Crop Germplasm Resources, Jinan 25000, China) 3 (Department of Respiratory Medicine, the Affiliated Hospital of Qingdao University, Qingdao 266003, China)

Online published: 2018-07-04

Supported by


摘要

以分离自市售商品、发酵萝卜和发酵面团的48株乳酸菌(lactic acid bacteria, LAB)为对象,评价其安全性和工业化应用前景。这48株菌中,有41株菌没有检测到腐胺、尸胺、组胺、酪胺、精胺和亚精胺的形成;有41株菌没有检测到溶解明胶的活性;有36株菌没有表现出明显溶解羊血的特性;有2株菌(QKL2和7)形成L-乳酸的光学纯度为91%,这2株菌不产生物胺、不溶解明胶且不对羊血产生明显溶解圈。这2株菌对低质量浓度(≤20 mg/L)的红霉素和阿莫西林表现为敏感,QKL7对大肠杆菌和酵母菌的抑制力要高于QKL2,但该菌对葡萄糖的理论利用率低。因此,试验获得了2株食用安全度高的LAB菌株,这2株菌形成的复合菌将是预防食品和饲草腐败的益生菌剂。

本文引用格式

闫肃, 李慧敏, 张晓冬, 等 . 不同食物来源乳酸细菌的安全性评价[J]. 食品与发酵工业, 2018 , 44(5) : 82 -89 . DOI: 10.13995/j.cnki.11-1802/ts.015919

Abstract

The aim of this study was to evaluate the safety of 48 lactic acid bacterial (LAB) strains that had previously been isolated from different food products such as yoghurt, goat's milk, cheese, spontaneous fermented green turnip and wheat flour, scallop, and oyster.The results showed that 41 LAB strains had no the tested biogenic amines (cadaverine, putrescine, histamine, tyramine, spermine, and spermidine), 41 LAB strains had no abilities to perform gelaune liquefaction, and 36 LAB strains could not or slightly dissolve sheep blood.However, among the tested strains, only QKL2 and QKL 7 produced the highly pure L-lactic acid (91%).Furthermore, these two strains showed sensitivity to low concentrations of two antibiotics (erythromycin and amoxicillin).QKL7 showed higher antimicrobial activity in comparison with QKL2, but it had lower glucose utilization due to the formation of carbon dioxide during fermentation.Based on these results, two strains, QKL2 and QKL 7, could be considered as interesting candidates for selection of starter cultures or probiotics for different applications.
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