以1株分离自酱醪的嗜盐四联球菌R23为出发菌株,采用紫外和等离子诱变的方法并通过高通量筛选获得了1株能够减少氨基甲酸乙酯(ethyl carbamate, EC)前体瓜氨酸并能高效利用精氨酸的突变株3-H9。与野生菌株R23相比,突变株3-H9在不利于EC前体代谢的条件下,利用精氨酸和瓜氨酸的能力均有显著提高。通过基因转录水平分析和对相关酶活测定发现,突变株3-H9精氨酸代谢途径中各基因转录水平和相应酶活性均高于野生菌R23。
Tetragenococcus halophilus R23, a soy sauce isolate, was mutagenized by UV mutagenesis and atmospheric and room temperature plasma (ARTP). By means of high throughput screening, a mutant strain, 3-H9, which exhibited enhanced capability in reduction of ethyl carbamate (EC) precursor citrulline and citrulline precursor, arginine was obtained. Compared with the wild strain R23, utilization of EC precursors citrulline and arginine under conditions that are not conducive to EC precursor metabolism by T. halophilus 3-H9, was greatly increased. Production of enzymes that are related to arginine and citrulline utilization was enhanced in T. halophilus 3-H9. Transcription level of corresponding genes involved in arginine metabolism in 3-H9 was detected to be higher than that in the wild strain R23.