诱变育种提高嗜盐四联球菌精氨酸和瓜氨酸利用能力

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  • 1(江南大学 生物工程学院,江苏 无锡,214122)2(工业生物技术教育部重点实验室,江苏 无锡,214122 ) 3(糖化学与生物技术教育部重点实验室,江苏 无锡,214122 )

网络出版日期: 2018-07-31

基金资助

国家自然科学基金(31371821,31771955)

Enhancement of arginine and citrulline utilization ability of Tetragenococcus halophilus by mutation breeding

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  • 1(School of Biotechnology, Jiangnan University, Wuxi 214122, China) 2(Key Laboratory of Industrial Biotechnology, Ministry of Education, Wuxi 214122, China) 3(The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China)

Online published: 2018-07-31

摘要

以1株分离自酱醪的嗜盐四联球菌R23为出发菌株,采用紫外和等离子诱变的方法并通过高通量筛选获得了1株能够减少氨基甲酸乙酯(ethyl carbamate, EC)前体瓜氨酸并能高效利用精氨酸的突变株3-H9。与野生菌株R23相比,突变株3-H9在不利于EC前体代谢的条件下,利用精氨酸和瓜氨酸的能力均有显著提高。通过基因转录水平分析和对相关酶活测定发现,突变株3-H9精氨酸代谢途径中各基因转录水平和相应酶活性均高于野生菌R23。

本文引用格式

雷庆子, 王博, 堵国成, 等 . 诱变育种提高嗜盐四联球菌精氨酸和瓜氨酸利用能力[J]. 食品与发酵工业, 2018 , 44(6) : 30 -36 . DOI: 10.13995/j.cnki.11-1802/ts.016327

Abstract

Tetragenococcus halophilus R23, a soy sauce isolate, was mutagenized by UV mutagenesis and atmospheric and room temperature plasma (ARTP). By means of high throughput screening, a mutant strain, 3-H9, which exhibited enhanced capability in reduction of ethyl carbamate (EC) precursor citrulline and citrulline precursor, arginine was obtained. Compared with the wild strain R23, utilization of EC precursors citrulline and arginine under conditions that are not conducive to EC precursor metabolism by T. halophilus 3-H9, was greatly increased. Production of enzymes that are related to arginine and citrulline utilization was enhanced in T. halophilus 3-H9. Transcription level of corresponding genes involved in arginine metabolism in 3-H9 was detected to be higher than that in the wild strain R23.
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