对以双水相萃取新技术提取ε-聚赖氨酸(ε-poly-L-lysine，ε-PL)作了较深入的探索研究。通过对多个双水相体系的筛选，确定了利用乙醇/硫酸铵双水相体系来萃取ε-PL的方案，得到的最佳萃取条件为：pH 9.5，乙醇/硫酸铵质量分数组成为20%/20%，分配系数和收率分别可以达到18.33和98.02%。进一步探究了多级萃取方式对ε-PL萃取效果的影响，发现3次萃取的效果最好，蛋白质和色素去除率分别达到85.66%和60.00%，ε-PL收率为96.15%。最后，收集经过3次萃取后的上相，利用超滤膜进行简单脱盐浓缩，产品的收率和纯度分别可以达到68.28%和92.39%。

An integrated fermentation-separation process for the production of ε-poly-L-lysine (ε-PL) was investigated. Through the screening of several aqueous two-phase systems, the extraction of ε -PL by ethanol/ammonium sulfate aqueous two-phase system was investigated in detail. The results indicated that the highest partition coefficient (18.33) and recovery (98.02%) of ε-PL were obtained with an aqueous two-phase system composed of 20% (w/w) ethanol and 20% (w/w) ammonium sulfate, pH9.5. The effect of multi-stage extraction was investigated. It was found that the effect of extraction for three times was the best, the removal rate of protein and pigment were 85.66% and 60.00%, respectively, and the yield of ε -PL was 96.15%. Finally, the top phase after three times of extraction was desalted and concentrated by ultrafiltration membrane, and the yield and purity of the product reached 68.28% and 92.39%, respectively.