从酒曲中筛选高产糖化酶菌株，并对筛选得到的菌株进行耐受性研究及固态发酵产酶条件的优化。耐受性试验结果表明：该菌株能耐受体积分数10%的酒精，在pH值4以上能较好生长，pH值3.5时生长受到明显抑制，在23～41 ℃范围内均能较好生长。通过单因素试验和正交试验，确定固态发酵产酶最佳条件为：糠壳添加量4%、接种量1.25%、初始pH值为5.5、料水比5∶3 (g∶mL)，在30 ℃下培养72 h，其糖化酶活性高达1 791.3 U/g。该菌株制作成浅盘曲：外观符合要求、试饭糖分含量24.76 g/100g。

The strain of high yield glycosylated enzyme was screened from the wine, and the tolerance study of the screened strains and the optimization of the enzymatic conditions of the solid fermentation were carried out. Tolerance test results showed that the strain was tolerant of 10% alcohol and the pH 4 above, and its growth was significantly inhibited at pH 3.5, while it could grow better at 23-41 ℃. By single factor test and orthogonal test, the best conditions for solid-state fermentation determined as follows: enzyme addition of 4%, bran shell quantity of 1.25%, initial pH value of 5.5, water ratio 5∶3 (g∶mL), cultivation at 30 ℃ for 72 h. Under these conditions, the saccharifying enzyme activity was up to 1791.3 U/g. The strain was made into a light disc. The appearance was in accordance with the requirements, and the content of sugar was 24.76 g/100g.