在酒精发酵不同时间接入植物乳杆菌,使葡萄汁酵母和植物乳杆菌混合发酵,平衡酒精发酵和苹果酸-乳酸发酵的作用。结果表明,于酒精发酵第1天接入植物乳杆菌,在发酵第15天,L-苹果酸含量即可由最初的5.40 g/L降为1.30 g/L左右,加快了发酵速率,缩短了发酵周期,且发酵后葡萄酒酸度和高级醇含量降低,酯含量明显提高,尤其是乳酸乙酯含量可以达到24.313 mg/L,提高了葡萄酒的风味质量。
This study aimed to explore the effects of inoculating Lactobacillus plantarum during alcoholic fermentation (AF) at different time to generate a co-fermentation of Saccharomyces uvarum and L. plantarum, to balance the effect of AF and malolactic fermentation (MLF) on wine. The results showed that L-malic acid metabolized from 5.04 g/L to 1.30 g/L on day 15 when L. plantarum was inoculated on the first day of AF, leading to increased fermentation rate and considerable reduction in overall fermentation duration. In addition, simultaneous inoculation not only reduced acidity and higher alcohol contents, but also improved the production of esters, especially when the ethyl lactate content increased to 24.313 mg/L, which improved the flavor quality of the wine.
[1] GONZÁLEZ-ARENZANA L, SANTAMARÍA P, LÓPEZ R, LÓPEZ-ALFARO I.Indigenous lactic acid bacteria communities in alcoholic and malolactic fermentations of tempranillo wines elaborated in ten wineries of la rioja (Spain) [J]. Food Research International, 2013,50(1), 438-445.
[2] LIU S Q. Malolactic fermentation in wine-Beyond deacidification [J]. Journal of Applied Microbiology, 2002, 92(4): 589-601.
[3] BARTOWSKY E J, BORNEMAN A R. Genomic variations of oenococcus oeni strains and the potential to impact on malolactic fermentation and aroma compounds in wine [J]. Applied Microbiology & Biotechnology, 2011,92(3):441-447.
[4] GAGNÉ S, LUCAS P M, PERELLO M C, et al. Variety and variability of glycosidase activities in an Oenococcus oeni strain collection tested with synthetic and natural substrates [J]. Journal of Applied Microbiology, 2011,110(1): 218-228.
[5] LI N, DUAN J, GAO D, LUO J, ZHENG R, BIAN Y, et al. Mutation and selection of Oenococcus oeni for controlling wine malolactic fermentation [J]. European Food Research & Technology, 2015,240(1): 93-100.
[6] 潘海燕. 苹果酒苹果酸乳酸发酵的研究[D]. 无锡:江南大学, 2004.
[7] 邱冬梅. 葡萄酒苹果酸-乳酸发酵的影响因素[J]. 江苏食品与发酵, 1996(3):15-17.
[8] 徐平. 用细菌噬菌体抑制葡萄酒中的细菌生长和苹果酸、乳酸发酵[J]. 微生物学杂志, 1988(1):80.
[9] 卜潇,薛雪,程静,等. 葡萄酒中植物乳杆菌苹果酸-乳酸发酵潜能评价[J]. 中国农业科学, 2017, 50(5):959-968.
[10] FUMI M D, SILVA A, KRIEGERWEBER S, et al. A new generation of malolactic starter cultures for high-ph wine [J]. Contributo, 2010.
[11] 王福荣. 酿酒分析与检测[M].北京:化学工业出版社,2005.
[12] ABRAHAMSE C E, BARTOWSKY E J. Timing of malolactic fermentation inoculation in Shiraz grape must and wine: influence on chemical composition [J]. World Journal of Microbiology & Biotechnology, 2012, 28(1):255-265.
[13] KLAMPFL C W, BUCHBERGER W, HADDAD P R. Determination of organic acids in food samples by capillary zone electrophoresis [J]. Journal of Chromatography A, 2000, 881(1):357-364.
[14] 杨春霞,苟春林,单巧玲. 葡萄酒酿造过程中有机酸变化规律研究[J]. 中国酿造, 2017, 36(4):83-86.
