研究报告

高产抑制大肠杆菌血凝性的胞外多糖的解淀粉芽孢杆菌

  • 蔡国林 ,
  • 冯文旭 ,
  • 刘逸凡 ,
  • 李晓敏 ,
  • 陆健
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  • 1(工业生物技术教育部重点实验室(江南大学),江苏 无锡,214122)
    2(粮食发酵工艺与技术国家工程实验室(江南大学),江苏 无锡,214122)
    3(江南大学 生物工程学院,江苏 无锡,214122)
博士,副研究员(陆健教授为通讯作者,E-mail:jlu@jiangnan.edu.cn)。

网络出版日期: 2019-03-25

基金资助

国家重点基础研究发展计划(973计划,2013CB 733602);高等学校学科创新引智计划(111计划)资助项目(111-2-06);江苏高校优势学科建设工程资助项目

A potential probiotic Bacillus amyloliquefaciens strain producing exopolysaccharide with anti-hemagglutination activity towards enterotoxigenic Escherichia coli

  • CAI Guolin ,
  • FENG Wenxu ,
  • LIU Yifan ,
  • LI Xiaomin ,
  • LU Jian
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  • 1(Key Laboratory of Industrial Biotechnology, Ministry of Education(Jiangnan University), Wuxi 214122, China)
    2(National Engineering Laboratory for Cereal Fermentation Technology(Jiangnan University), Wuxi 214122, China)
    3(School of Biotechnology, Jiangnan University, Wuxi 214122, China)

Online published: 2019-03-25

摘要

产肠毒素大肠杆菌(enterotoxigenic Escherichia coli, ETEC)是一类引起断奶仔猪腹泻的重要病原菌,筛选获得高产抑制ETEC血凝性胞外多糖的芽孢杆菌可以有效预防这类动物疾病。该研究从健康动物粪便中分离获得芽孢杆菌,比较分析芽孢杆菌胞外多糖产量及其抑制ETEC血凝性的能力,最终筛选获得1株多糖产量大于20 g/L,多糖质量浓度在4 mg/mL时就能抑制ETEC血凝性的芽孢杆菌JN4。体外益生潜力评价发现,其芽孢生成率高,萌发率高,具有很好的耐受人工肠、胃液能力,对大部分常规抗生素敏感。通过生理生化及16 S rRNA序列分析,鉴定其为解淀粉芽孢杆菌JN4。

本文引用格式

蔡国林 , 冯文旭 , 刘逸凡 , 李晓敏 , 陆健 . 高产抑制大肠杆菌血凝性的胞外多糖的解淀粉芽孢杆菌[J]. 食品与发酵工业, 2019 , 45(5) : 14 -18 . DOI: 10.13995/j.cnki.11-1802/ts.018363

Abstract

Enterotoxigenic Escherichia coli (ETEC) is a leading bacterial cause of diarrhea in weaned piglets. An effective prevention and treatement of ETEC caused diarrhea is to isolate a potential probiotic Bacillus strain which could produce exopolysaccharide (EPS) with anti-hemagglutination activity towards ETEC. Bacillus amyloliquefaciens JN4 was isolated from healthy piglets faeces. When cultured with LB medium containing 100 g/L sucrose, it could produce more than 20 g/L EPS. The EPS showed anti-hemagglutination activity with a titer of 4 mg/mL, lower than that of EPS from other Bacillus strains. The spores of JN4 were tolerant to gastric and small intestinal juice, indicated its capable to survive in the intestine, and the JN4 strain was sensitive to most commercial antibiotics.

参考文献

[1] GAGGIA F, MATTARELLI P, BIAVATI B. Probiotics and prebiotics in animal feeding for safe food production [J]. International Journal of Food Microbiology, 2010, 141:S15-S28.
[2] JOHN J, ROEDIGER K, SCHROEDL W, et al. Development of intestinal microflora and occurrence of diarrhea in sucking foals: effects of Bacillus cereus var. toyoi supplementation [J]. BMC Veterinary Research, 2015, 11:34.
[3] CUTTING S M. Bacillus probiotics [J]. Food Microbiology, 2011, 28(2):214-220.
[4] SANTOSO U, TANAKA K, OHTANI S, et al. Effect of fermented product from Bacillus subtilis on feed conversion efficiency, lipid accumulation and ammonia production in broiler chicks [J]. Asian-Australasian Journal of Animal Sciences, 2001, 14(3):333-337.
[5] DILNA S V, SURYA H, ASWATHY R G, et al. Characterization of an exopolysaccharide with potential health-benefit properties from a probiotic Lactobacillus plantarum RJF(4) [J]. LWT-Food Science and Technology, 2015, 64(2):1 179-1 186.
[6] SALAZAR N, GUEIMONDE M, REYES-GAVILAN C G, et al. Exopolysaccharides produced by Lactic Acid Bacteria and Bifidobacteria as fermentable substrates by the intestinal microbiota [J]. Critical Reviews in Food Science and Nutrition, 2016, 56(9):1 440-1 453.
[7] POLAK-BERECKA M, SZWAJGIER D, WASKO A. Biosorption of Al(+3) and Cd(+2) by an exopolysaccharide from Lactobacillus rhamnosus[J]. Journal of Food Science, 2014, 79(11):T2 404-T2 408.
[8] 梁增澜,李超,王艳萍. 乳酸菌胞外多糖免疫活性的研究进展[J]. 食品与发酵工业, 2018,44(2):266-272.
[9] YANG H X, DENG J J, YUAN Y, et al. Two novel exopolysaccharides from Bacillus amyloliquefaciens C-1: Antioxidation and effect on oxidative stress [J]. Current Microbiology, 2015, 70(2):298-306.
[10] NGUYEN ATV, NGUYEN DV, TRAN MT, et al. Isolation and characterization of Bacillus subtilis CH16 strain from chicken gastrointestinal tracts for use as a feed supplement to promote weight gain in broilers [J]. Letters in Applied Microbiology, 2015, 60(6):580-588.
[11] 李洁,窦文芳,李会,等. 胶质芽孢杆菌胞外多糖的制备及流变学特性[J]. 食品与发酵工业, 2013, 39(1):1-5.
[12] WANG Y, GANZLE M G, SCHWAB C. Exopolysaccharide synthesized by Lactobacillus reuteri decreases the ability of enterotoxigenic Escherichia coli to bind to porcine erythrocytes[J]. Applied Environmental Microbiology, 2010, 76(14):4 863-4 866.
[13] 李善仁,陈济琛,蔡海松,等. 三株芽孢杆菌作为益生菌的生物特性[J]. 营养学报, 2010, 32(1):75-78.
[14] 严涛,朱建国,姜甜,等. 一株凝结芽孢杆菌的分离筛选及产孢条件优化[J]. 微生物学通报, 2018, 45(2):238-249.
[15] NORDESTE R, TESSEMA A, SHARMA S, et al. Molecules produced by probiotics prevent enteric colibacillosis in pigs [J]. BMC Veterinary Research, 2017, 13:335.
[16] FAIRBROTHER J M, NADEAU E, GYLES C L. Escherichia coli in postweaning diarrhea in pigs: an update on bacterial types, pathogenesis, and prevention strategies [J]. Animal Health Research Reviews, 2005, 6(1):17-39.
[17] SHOAF K, MULVEY G L, ARMSTRONG G D, et al. Prebiotic galactooligosaccharides reduce adherence of enteropathogenic Escherichia coli to tissue culture cells [J]. Infection and Immunity, 2006, 74(12):6 920-6 928.
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