食品与发酵工业 >

2019 , Vol. 45 >Issue 5: 218 - 223

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.017926

分析与检测

基于磁性纳米材料和适配体的荧光传感器检测牛奶中黄曲霉毒素M1

  • 郭婷 ,
  • 林淑凤 ,
  • 马良 ,
  • 谭红霞 ,
  • 张宇昊
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  • (西南大学 食品科学学院,重庆,400715)
博士,讲师(马良教授为通讯作者,E-mail:zhyhml@163.com)。

网络出版日期: 2019-03-25

基金资助

重庆市技术创新与应用示范项目(cstc2018jscx-msyb0804);中央高校基本科研业务费专项资金(SWU117005,XDJK2017B042)

收起

A fluorescent biosensor based on magnetic nanoparticles and aptamer for detecting AFM1 in milk

  • GUO Ting ,
  • LIN Shufeng ,
  • MA Liang ,
  • TAN Hongxia ,
  • ZHANG Yuhao
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  • (College of Food Science, Southwest University, Chongqing, 400715, China)

Online published: 2019-03-25

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摘要

根据荧光共振能量转移原理,利用磁性纳米材料的磁性分离技术及荧光猝灭能力,构建了基于磁性纳米材料和适配体的荧光传感器,用于高灵敏检测牛奶中黄曲霉毒素M1(aflatoxin M1, AFM1)。标记羧基荧光素(carboxy-fluorescein, FAM)的适配体通过静电作用吸附在Fe3O4磁性纳米颗粒表面,并与Fe3O4发生能量共振转移,导致荧光猝灭;当体系中存在AFM1时,适配体与AFM1特异性识别并形成折叠结构,适配体从Fe3O4磁性纳米颗粒表面脱附,使得荧光信号恢复,据此可实现对FAM1的定量检测。该研究对所制备的Fe3O4磁性纳米颗粒进行表征,透射电镜结果表明,Fe3O4磁性纳米颗粒粒径在10～15 nm。在优化的实验条件下,该传感器的线性范围为0.05～0.70 μg/L,检测限为0.02 μg/L。利用荧光传感器检测牛奶中AFM1的回收率为82.5%～102.3%。

关键词： 核酸适配体; 黄曲霉毒素M1; Fe3O4磁性纳米颗粒; 荧光传感器

本文引用格式

郭婷 , 林淑凤 , 马良 , 谭红霞 , 张宇昊 . 基于磁性纳米材料和适配体的荧光传感器检测牛奶中黄曲霉毒素M1[J]. 食品与发酵工业, 2019 , 45(5) : 218 -223 . DOI: 10.13995/j.cnki.11-1802/ts.017926

Abstract

Based on the mechanism of fluorescence resonance energy transfer (FRET), a fluorescence biosensor was constructed by magnetic nanoparticles with abilities of magnetic separation and fluorescence quenching as well as the aptamer to highly sensitively detect aflatoxin M1 (AFM1) in milk. The aptamer labelled with carboxy-fluorescein (FAM) adsorbed to the surface of Fe3O4 magnetic nanoparticles by electrostatic interaction, followed by FRET from FAM-aptamer to Fe3O4, resulting in fluorescent quenching. When AFM1 was present, FAM-aptamer identified AFM1 specifically and desorbed from the surface of Fe3O4 after forming the aptamer-AFM1 complex, resulting the recovery of the fluorescent signal. Based on this, AFM1 could be quantitatively measured. In this study, the prepared Fe3O4 magnetic nanoparticles were characterized. Transmission electron microscopy analysis showed that the size of Fe3O4 was 10-15 nm. Under optimal conditions, the linear range of detection of the aptamer was 0.05-0.70 μg/L, and the detection limit was 0.02 μg/L. The recovery rate of using fluorescence biosensor to detect AFM1 in milk was 82.5%-102.3%. This developed strategy provides a new thought for constructing highly sensitive and rapid detection method.

Key words： aptamer; aflatoxin M1; Fe3O4 magnetic nanoparticles; fluorescence sensor

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