生产与科研经验

三相萃取体系分离富集螺旋藻多糖及其结构特征分析

  • 罗光宏 ,
  • 马明辉 ,
  • 张喜峰 ,
  • 杨生辉 ,
  • 王丹霞 ,
  • 陈天仁
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  • 1(河西学院,甘肃省微藻技术创新中心,甘肃 张掖,734000)
    2(甘肃省河西走廊特色资源利用重点实验室,甘肃 张掖,734000)
    3(河西学院农业与生物技术学院,甘肃 张掖,734000)
硕士,教授(张喜峰副教授为通讯作者,E-mail:curi ouslysxsd@163.com)。

收稿日期: 2018-05-16

  网络出版日期: 2019-04-18

基金资助

国家自然科学基金(11665011);甘肃省高校协同创新科技团队支持计划(项目编号2017C-17);2019甘肃省微藻技术创新中心建设项目;河西学院第八批大学生科技创新项目(135)

Three-phase partitioning for efficient extraction and separation of polysaccharides from Spriulina platensis and its structural characterization

  • LUO Guanghong ,
  • MA Minghui ,
  • ZHANG Xifeng ,
  • YANG Shenghui ,
  • WANG Danxia ,
  • CHEN Tianren
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  • 1(Gansu Innovation Technology Innovation Center for Microalgae, Hexi University, Zhangye 734000, China)
    2 (Key Laboratory of Hexi Corridor Resources Utilization of Gansu, Zhangye 734000, China)
    3 (College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China)

Received date: 2018-05-16

  Online published: 2019-04-18

摘要

采用简单、有效、绿色的三相萃取体系分离富集螺旋藻多糖,分别研究了(NH4)2SO4质量分数、叔丁醇加入量、pH、萃取温度、萃取时间对多糖得率的影响,并对萃取后多糖结构特征进行了分析;结果表明,(NH4)2SO4质量分数为20%,叔丁醇加入量为15 mL,体系pH为7.0,在35 ℃条件下,萃取30 min后,螺旋藻多糖得率为(9.25±0.42)%;红外光谱表明其具有多糖的特征吸收峰,紫外-可见波长扫描显示其不含蛋白质和核酸,采用凝胶渗透色谱测定多糖重均分子质量为3.597×104,其单糖物质量组成为n(鼠李糖):n(甘露糖):n(葡萄糖)=5.14:5.78:81.37;该法可为天然产物活性成分提取和纯化提供借鉴作用。

本文引用格式

罗光宏 , 马明辉 , 张喜峰 , 杨生辉 , 王丹霞 , 陈天仁 . 三相萃取体系分离富集螺旋藻多糖及其结构特征分析[J]. 食品与发酵工业, 2019 , 45(6) : 147 -152 . DOI: 10.13995/j.cnki.11-1802/ts.017786

Abstract

Three-phase partitioning (TPP), which is a simple, efficient, and green bioseparation technique, was used to extract and separate polysaccharides from Spriulina platensis (PSP). The effect of mass fraction of (NH4)2SO4, amount of t-butanol, pH, temperature, and extraction time on the yield of PSP were studied. The structural feature of extracted PSP were also analyzed. The optimal parameters with a high yield of (9.25±0.42)% were as follows: mass fraction of (NH4)2SO4, 20.0%; amount of t-butanol, 15 mL; pH 7.0; temperature, 35 ℃; and extraction time, 30 min. The results of IR showed that PSP had the characteristic absorption peaks of polysaccharide, UV wavelength scanning result showed that PSP do not contain protein and nucleic acids. The weight average molecular of PSP determined by gel permeation chromatography can reach 3.597×104, which is composed of Rhamnose, mannose, and glucose in a molar ratio of 5.14:5.78:81.37, respectively. Hence, TPP was an excellent efficient to concurrent extraction and purification polysaccharides.

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