高密度培养(high cell density cultivation,HCDC)是酵母生产实现高效高产的途径。文中以菌体量、菌体得率和生产强度为评价指标,探讨了拟指数和DO-stat两种补料策略的优缺点,提出了1种两阶段补料策略:在21 h的分批培养结束后,首先采用比生长速率为0.03 h-1的拟指数补料,然后在溶氧跌到20%以下后将补料方式切换为DO 40%的DO-stat补料。结果显示,两阶段补料策略达到了菌体量123.57 g/L、总菌体得率0.51 g/g、生产强度1.15 g/(L·h)。与拟指数补料策略相比,菌体量提高了23.8%、菌体得率提高 6.25%;与DO-Stat补料策略相比,培养周期缩短20.4%、生产强度提高了14.5%。研究结果表明,两阶段补料策略可以有效地提高酵母生长能力。
High cell density cultivation (HCDC) is a way to achieve efficient and high yeast production yield. Two feeding strategies, quasi-exponential and DO-Stat, were evaluated according to the dry cell weight, yield, and productivity. Based on the results, a two-step feeding strategy was developed, which started with quasi-exponential at specific growth rate of 0.03 h-1 after 21 h batch cultivation. The feeding strategy was then switched to DO-Stat with 40% DO when the dissolved oxygen fell to 20%. Implementation of the two-step feeding strategy resulted in 123.57 g/L dry cell weight, total yield of 0.51 g/g, and 1.15 g/(L·h) productivity. Compared with quasi-exponential feeding strategy, the dry cell weight and the yield of the two-step feeding strategy increased by 23.8% and 6.25%, respectively. Compared with DO-Stat feeding strategy, the production period shortened by 20.4% and the productivity increased by 14.5%. The results indicated that the two-step feeding strategy can effectively improve the growth capacity of yeast, which provides valuable reference and significant guidance for industrial production of yeast.
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