生产与科研经验

蛹虫草SOD高活性菌株筛选及液体培养条件优化

  • 马婕馨 ,
  • 蔡程山 ,
  • 高苏 ,
  • 方晔晨 ,
  • 赵国柱
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  • 1(北京林业大学 生物科学与技术学院,北京,100083)
    2(中国食品发酵工业研究院,中国工业微生物菌种保藏中心,北京,100015)
硕士研究生(赵国柱副教授为通讯作者,E-mail:zhaogz@im.ac.cn)。

收稿日期: 2019-01-14

  网络出版日期: 2019-06-17

基金资助

国家自然科学基金(31570019)

Screening and fermentation optimization of Cordyceps militaris having high SOD activity

  • MA Jiexin ,
  • CAI Chengshan ,
  • GAO Su ,
  • FANG Yechen ,
  • ZHAO Guozhu
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  • 1(College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China)
    2(China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China)

Received date: 2019-01-14

  Online published: 2019-06-17

摘要

建立了一种较为系统的蛹虫草液体发酵产SOD的培养条件。以4种来源蛹虫草(Cordyceps militaris)菌株YCC-B、YCC-C、YCC-W、YCC-Y为研究对象,以生物量和SOD活性为指标,筛选出SOD高活性菌株。之后优化SOD高活性菌株的液体发酵条件,以生物量、SOD清除超氧阴离子能力(清除率)、酶活力和比活力为测定指标,选取接种量、装液量和pH展开3因素3水平的正交实验。经筛选,得到SOD高活性菌株为YCC-W。通过优化YCC-W的液体发酵条件,得出最佳组合为接种量4%,装液量100 mL/250 mL和pH 5.5。SOD清除率可提高41.17%,达到54.84%;SOD酶活力可提高42.04%,达到19.74 U/mL;比活力可提高44.23%,达到56.34 U/mg。该研究提供了通过蛹虫草菌丝体发酵产SOD的方法,在替代传统动物血液获取SOD上具有一定的应用前景;通过系统优化发酵条件SOD活性得到显著提升,通过继续扩大实验规模,有望用于SOD的规模化生产。

关键词: 蛹虫草; SOD; 发酵

本文引用格式

马婕馨 , 蔡程山 , 高苏 , 方晔晨 , 赵国柱 . 蛹虫草SOD高活性菌株筛选及液体培养条件优化[J]. 食品与发酵工业, 2019 , 45(10) : 122 -128 . DOI: 10.13995/j.cnki.11-1802/ts.019951

Abstract

To establish a fermentation process to produce SOD by Cordyceps militaris, the biomass and SOD activity of the mycelia from four strains of C. militaris,YCC-B, YCC-C, YCC-W, and YCC-Y, were examined. Of which, C. militaris YCC-W produced the highest activity of SOD. The optimized fermentation condition for producing SOD from YCC-W was as follows: 4% inoculum, 100 mL working volume in a 250 mL flask, and at pH=5.5. Under this condition, the SOD activity was 19.74 U/mL (increased 42.04%) and the specific activity was 56.34 U/mg (enhanced by 44.23%). Besides, the removal rate of SOD increased by 41.17% and reached 54.84%. In conclusion, this study provides a new method for producing SOD by C. militaris fermentation, which shows a certain application prospect in replacing traditional animal blood to obtain SOD.

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