食品与发酵工业 >

2019 , Vol. 45 >Issue 21: 38 - 45

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.021589

研究报告

高产胞外多糖植物乳杆菌筛选及其发酵工艺优化

  • 张文平 ,
  • 赵英杰 ,
  • 罗晟 ,
  • 程新
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  • 江西农业大学 生物科学与工程学院,江西 南昌,330045
硕士研究生(程新副教授为通讯作者,E-mail:xincheng@jxau.edu.cn)。

收稿日期: 2019-07-07

  网络出版日期: 2019-11-15

基金资助

国家星火计划项目(2015GA730012);江西省教育厅科技计划项目(GJJ180211)

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Screening of Lactobacillus plantarum with higher yield of exopolysaccharides andoptimization of fermentation conditions

  • ZHANG Wenping ,
  • ZHAO Yingjie ,
  • LUO Sheng ,
  • CHENG Xin
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  • College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China

Received date: 2019-07-07

  Online published: 2019-11-15

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摘要

为获得高产胞外多糖的乳酸菌,采用菌落拉丝法和硫酸-苯酚法相结合的手段筛选获得1株高产胞外多糖的乳酸菌LPC-1,初步鉴定为Lactobacillus plantarum。以胞外多糖产量为指标,采用单因素和响应面实验对发酵工艺进行优化。优化的培养条件为:蔗糖30 g/L、大豆蛋白胨10 g/L、发酵时间24 h、温度30 ℃、初始pH值6.5、接种量4%(体积分数)。通过Plackett-Burman实验确定温度、pH、柠檬酸氢二铵为显著因子,结合中心组合实验及响应面分析,确定最优发酵工艺条件为温度32 ℃、pH值6.7、柠檬酸氢二铵3 g/L,在此条件下发酵测得实际产量为2 064.69 mg/L,与预测值基本吻合,与优化前相比增加了48.64%,为乳酸菌胞外多糖的规模化生产提供了依据。

关键词： 植物乳杆菌; 胞外多糖; 条件优化; Plackett-Burman设计; 响应面法

本文引用格式

张文平 , 赵英杰 , 罗晟 , 程新 . 高产胞外多糖植物乳杆菌筛选及其发酵工艺优化[J]. 食品与发酵工业, 2019 , 45(21) : 38 -45 . DOI: 10.13995/j.cnki.11-1802/ts.021589

Abstract

In order to obtain the lactic acid bacteria (LAB) with high exopolysaccharide (EPS)-producing ability, a strain of LPC-1 was finally screened by observing whether they could form sticky colonies and sulfuric acid-phenol method, which was identified as Lactobacillus plantarum. Using the yield of extracellular polysaccharide as an indicator, the fermentation process was optimized by single factor and response surface tests. The results showed that the optimal culture conditions were: 30 g/L sucrose and 10 g/L soybean peptone, inoculation amount of 4%, fermented at 30 ℃ and pH 6.5 for 24 h. The production of EPS was significantly affected by temperature, pH and concentration of diammonium citrate as determined by the Plackett-Burman test. Combined with the central combination test and response surface analysis, the optimal fermentation conditions were 32 ℃, pH 6.7 and 3 g/L diammonium citrate. Under this condition, the EPS yield was 2 064.69 mg/L, which was consistent with the predicted value and increased by 48.64% compared with that before optimization. These results provided a basis for the scale production of EPS by LAB.

Key words： Lactobacillus plantarum; exopolysaccharides; optimization; Plackett-Burman design; response surface methodology

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