研究报告

Fur过表达对大肠杆菌血红素合成的影响

  • 李蒙蒙 ,
  • 王雨萱 ,
  • 唐蕾
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  • 1 (工业生物技术教育部重点实验室(江南大学),江苏 无锡,214122)
    2 (江南大学 生物工程学院,江苏 无锡,214122)
硕士研究生(唐蕾教授为通讯作者,E-mail:ltang@jiangnan.edu.cn)。

收稿日期: 2019-04-11

  网络出版日期: 2019-09-23

基金资助

111引智计划(111-2-06);国家轻工技术与工程一流学科自主课题资助(LITE2018-27)

Effects of fur overexpression on heme synthesis in Escherichia coli

  • LI Mengmeng ,
  • WANG Yuxuan ,
  • TANG Lei
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  • 1 (Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China)
    2 (School of Biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2019-04-11

  Online published: 2019-09-23

摘要

为了阐明铁摄取调节子基因fur对大肠杆菌血红素合成的影响,对fur基因进行了同源过表达,考察了菌体生长、铁离子浓度及血红素合成变化,并分析了血红素合成途径关键基因的表达水平。结果表明,原始菌株在限铁条件下的血红素量比在非限铁条件下的血红素量下降2.54倍;fur过表达对菌体的生长有抑制作用,胞内的铁离子减少;非限铁条件下,过表达菌株Eco/pEF的血红素量比原始菌株下降了3.55倍;而过表达菌株Eco/pEF在限铁条件下的血红素量比在非限铁条件下的血红素量增加3.10倍。fur过表达引起hemA下调,而gltXhemBhemC分别上调4.93倍、4.61倍和9.39倍;菌株Eco/pEF在限铁条件下hemBhemG分别上调了5.59倍和4.49倍。虽然fur过表达使血红素合成途径基因表达水平上调,但并未导致血红素大量合成。该结果为通过铁离子摄取调控胞内血红素的水平提供了新的理论支持。

本文引用格式

李蒙蒙 , 王雨萱 , 唐蕾 . Fur过表达对大肠杆菌血红素合成的影响[J]. 食品与发酵工业, 2019 , 45(16) : 18 -25 . DOI: 10.13995/j.cnki.11-1802/ts.020834

Abstract

In order to elucidate the effects of iron uptake regulator gene fur on heme synthesis in Escherichia coli, fur was homogenously overexpressed. The growth, the concentration of iron ions and the changes in heme synthesis and the expression level of key genes in heme synthesis pathway were analyzed. The results showed that the heme content in the original strain under iron limited condition was 2.54 times lower than that under non-iron limited condition. Overexpression of fur inhibited the growth and decreased the concentration of intracellular iron ions. Under non-iron limited condition, the heme content in fur overexpressed strain Eco/pEF decreased by 3.55 times compared with that of the original strain and the heme content in Eco/pEF under iron limited condition increased by 3.10 times compared with that under non-iron limited condition. Overexpression of fur down-regulated hemA, while gltX, hemB and hemC were up-regulated by 4.93, 4.61 and 9.39 times, respectively. Under iron limited condition, hemB and hemG in Eco/pEF were up-regulated by 5.59 and 4.49 times, respectively. Although fur overexpression up-regulated the expression levels of the genes involved in heme synthesis pathway, heme content did not increase significantly. These results provide theoretical support for regulating intracellular heme levels by iron ion uptake.

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