为了节约发酵液灭菌过程中蒸汽的用量,用臭氧灭菌水代替多糖发酵液中部分水的灭菌,并研究对发酵过程和结果的影响。结果表明,在水中臭氧质量浓度达到3、4、5 mg/L时,自来水达到能够满足发酵使用的无菌状态的时间分别为120、60、40 min。用臭氧灭菌好的自来水代替发酵液中50%的水,分别进行摇瓶和中试发酵,与全部采用蒸汽灭菌的发酵液发酵相比,不论是发酵过程中还原糖和多糖的变化趋势还是发酵结束时多糖的产量,采用臭氧灭菌的发酵液都没有受到影响。对10 m3发酵罐灭菌的蒸汽耗量和冷却水进行计算,采用臭氧灭菌发酵液可以节约蒸汽47.3%,减少冷却水用量5 835 kg/罐。该研究表明,臭氧灭菌水技术可以为发酵液灭菌节约大量蒸汽和冷却水,为发酵行业的节能发展提供新方法。
In order to save the amount of steam used in fermentation broth sterilization, ozone sterilized water was used to replace part of the water in polysaccharide fermentation broth and the influences on fermentation process and results were studied. The results showed that 3, 4 and 5 mg/L ozone could sterilize tap water for fermentation in 120, 60, and 40 min, respectively. With 50% water replaced by ozone-sterilized tap water in the fermentation broth, there were no changes in reducing sugar and polysaccharides during or at the end of the fermentation, both in shake flask and pilot-scale fermentation, compared with steam-sterilized fermentation broth. Moreover, it was found that ozone-sterilized fermentation broth could save 47.3% steam and reduce 5 835 kg cooling water in a 10 m3 fermentor. Therefore, using ozone sterilized water can save a large amount of steam and cooling water, which provides a new method for energy-saving in fermentation industry.
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