采用高效液相色谱、质谱和核磁共振分析,对液态发酵红曲黄色素粗提物色素组分进行分离纯化和分子结构鉴定,确定其化学结构式。结果表明,红曲黄色素有2种主要色素成分,组分A和组分B,两者在分析型液相色谱上分离度达到2.51。进一步将分析型液相分离条件在制备型液相上进行放大并调整,收集得到组分A和组分B,纯度分别为98.95%和99.02%,达到标准品的纯度。通过质谱分析结合一维及二维核磁共振,确定组分A的相对分子质量为358,为红曲素(C21H26O5),组分B的相对分子质量为332,为Monaphilone B(C20H28O4),A和B的显色性能来自于其分子结构中的α, β-不饱和酮结构。
The purpose of this study was to isolate Monascus yellow pigments and to study its chemical structure. Components of Monascus yellow pigments from fermentation broth were isolated, purified and identified by high-performance liquid chromatography (HPLC), mass spectrometry (MS) and Nuclear magnetic resonance (NMR). Two major components showed on HPLC spectrum as two adjacent peaks, namely A and B. Resolution between A and B was 2.51. Then A and B were isolated using preparative HPLC with a few adjustments. High purity of A (98.95%) and B (99.02%) were obtained. Combing data from MS with 1D and 2D NMR analysis, A and B were elucidated as Monascin (C21H26O5, molecular weight 358) and Monaphilone B (C20H28O4, molecular weight 332), respectively. The yellow pigment comes from its α, β-unsaturated ketone structures.
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