为进一步提高粪肠乳酸球菌合成γ-氨基丁酸(GABA)的产量,在250 mL摇瓶水平上考察了转速(0和100 r/min)对菌体生长和产物合成的影响。通过分析不同转速下发酵曲线和动力学参数,提出两阶段转速调控策略,并对影响此调控策略的发酵条件(阶段时间、装液量及转速)进行正交优化。72 h、0 r/min产量最高,稳定期、100 r/min生物量最高。优化后发酵条件为装液量100 mL,在发酵前期(0~12 h)控制转速150 r/min,发酵后期(12~72 h)控制转速0 r/min;在此基础上,GABA产量达到(6.713±0.135) g/L,比优化前(0和100 r/min)分别提高了12.2%与60.1%。两阶段转速控制策略可以有效提高粪肠乳酸球菌合成GABA的能力,对GABA工业化生产具有重要的参考价值和指导意义。
In order to further improve the production of GABA synthesized by Enterococcus lactic acid faecalis, the effects of rotational speed (0 and 100 r/min) on the growth and GABA production of bacteria were investigated at a 250 mL shake flask level. By analyzing the fermentation curve and kinetic parameters at different rotational speeds, a two-stage rotational speed regulation strategy was proposed, and the fermentation conditions (stage time, liquid volume and rotational speed) that affected this strategy were optimized. It was found that the yield at 72 h and 0 r/min was the highest, and the biomass at 100 r/min in stable periods was the highest. The optimized fermentation conditions were as follows: the liquid volume was 100 mL, rotational speed was 150 r/min at the first stage of fermentation (0-12 h), then switched to 0 r/min at the second stage (12-72 h). On this basis, the production of GABA reached (6.713 ± 0.135) g/L, which was 12.2 % and 60.1 % higher than that before optimization at 0 and 100 r/min, respectively. Therefore, two-stage rotational speed control strategy can effectively improve the ability of E. lactic acid faecalis to synthesize GABA, which has an important reference value and guiding significance for industrial production of GABA.
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