为研究干腌食盐用量对南京盐水鸭特征风味的影响,采用固相微萃取-气质联用法(SPME-CG-MS)测定挥发性风味物质,以市售盐水鸭为对照,结合气味活度值(OAV)确定南京盐水鸭特征风味成分,采用PCA法分析干腌盐量(4%、6%、8%,W/W)与盐水鸭特征风味物质间的相关性。结果表明,南京盐水鸭的特征风味物质为戊醛、1-己醇、己醛、甲酸乙酯、2-庚酮、庚醛、1-辛烯-3-醇、2,3-辛二酮、1-辛醇、辛醛、壬醛、2-辛烯醛、2,4-壬二烯醛、2,4-癸二烯醛、2-戊基呋喃共15种。随着干腌盐量的提高,盐水鸭特征风味物质的含量有显著增加。4%盐量不利于盐水鸭特征风味的形成,与6%盐量盐水鸭相关性较高的特征风味物质有甲酸乙酯、庚醛、2,4-壬二烯醛,与8%盐量盐水鸭相关性较高的特征风味物质有1-辛醇、壬醛。该结果可为传统盐水鸭制品的低盐化及风味稳定化提供依据和参考。
To investigate the effects of dry-cured salt amount on the characteristic flavor of Nanjing boiled-salted duck, solid-phase micro-extraction-gas-chromatography-mass spectrometry(SPME-CG-MS) was used to determine the volatile flavor compounds, and the characteristic flavor component was determined by odor activity value (OAV) using commercial boiled-salted ducks as control, and PCA method was used to analyze the correlation between dry-cured salt amount (4%, 6%, 8%, w/w) as well as the characteristic flavor compounds in boiled-salted ducks. The results showed that there were 15 kinds of characteristic flavor compounds in Nanjing boiled-salted duck, including pentanal, 1-hexanol, hexanal, ethyl formate, 2-heptanone, heptanal, 1-octene-3-ol, 2,3-octanedione, 1-octanol, octanal, nonanal, 2-octenal, 2,4-nonadienal, 2,4-decadienal, and 2-pentylfuran. Moreover, the content of characteristic flavor substances increased significantly with the increase of dry-cured salt amount. 4% salt was not conducive to the formation of characteristic flavor of boiled-salted duck. The characteristic flavor substances had high correlations with 6% salt group were ethyl formate, heptanal and 2,4-nonadienal, while 1-octanol and nonanal were more correlated with 8% salt group. In conclusion, the results provide a theoretical basis and reference for lowing salt content and stabilizing flavor of traditional boiled-salted duck products.
[1] 谢伟, 徐幸莲,周光宏. 不同生产工艺对盐水鸭风味的影响[J]. 食品科学, 2010, 31(8):110-115.
[2] 谢伟, 刘登勇,徐幸莲,等. 不同卤水复卤对盐水鸭风味的影响[J]. 江苏农业学报, 2010, 26(3):664-666.
[3] 刘汉文, 颜秀花,杨文萍. 盐水鸭生产工艺的研究[J]. 食品科技, 2008, 33(8):81-83.
[4] 周慧敏, 张顺亮,成晓瑜,等. 食盐用量对风干猪肉挥发性风味物质的影响[J]. 肉类研究, 2017, 31(4):23-28.
[5] WANG Y, JIANG Y T, CAO J X, et al.Study on lipolysis-oxidation and volatile flavour compounds of dry-cured goose with different curing salt content during production[J]. Food Chemistry, 2016, 190:33-40.
[6] VAN G L J. Complilations of Odour Threshold Values in Air,Water and Other Media[M].Van Setten Kwadraat: Houten, The Netherlands, 2003.
[7] 何聪聪, 苏柯冉,刘梦雅,等. 基于AEDA和OAV值确定西瓜汁香气活性化合物的比较[J]. 现代食品科技, 2014, 30(7): 279-285.
[8] MOTTRAM D S. Flavour formation in meat and meat products: a review[J]. Food Chemistry, 1998, 62(4):415-424.
[9] ELMORE J S, MOTTRAM D S, ENSER M, et al. Effect of the polyunsaturated fatty acid composition of beef muscle on the profile of aroma volatiles[J]. Journal of Agricultural and Food Chemistry, 1999, 47(4):1 619-1 625.
[10] FORSS D A.Odor and flavor compounds from lipids[J]. Progress in the Chemistry of Fats & Other Lipids, 1973, 13:177-258.
[11] 江新业, 宋焕禄,华永兵,等. 北京鸭/樱桃谷鸭肉汤中香味物质的比较[J]. 食品与发酵工业, 2004, 30(1):21-24.
[12] WURZENBERGER M, GROSCH W. Stereo chemistry of the cleavage of the 10-hydroperoxide isomer of linoleic acid to 1-octen-3-ol by a hydroperoxide lyase from mushrooms (psalliota bispora)[J]. Biochimica Et Biophysica Acta, 1984, 795(1):163-165.
[13] CALKINS C R, HODGEN J M. A fresh look at meat flavor[J]. Meat Science, 2007, 77(1): 8 053-8 063.
[14] 李聪, 徐宝才,李世保,等. 市售盐水鸭挥发性风味物质研究分析[J]. 现代食品科技, 2016, 12:356-364.
[15] 刘源, 徐幸莲,王锡昌,等. 同时蒸馏萃取法分析鸭肉挥发性风味[J]. 食品与机械, 2007, 23(4):15-17.
[16] 高先楚, 王锡昌,顾赛麒,等. 中华绒螯蟹性腺加热熟制前后挥发性成分和脂肪酸组成分析[J]. 现代食品科技, 2014(9):265-274.
[17] SHAHIDI F, RUBIN L J, D'SOUZA L A, et al. Meat flavor volatiles: A review of the composition, techniques of analysis, and sensory evaluation[J]. Critical Reviews in Food Science & Nutrition, 1986, 24(2):141-243.
[18] 汪新洁, 刘凤杰,郑俊,等. 不同挤压膨化温度对燕麦片风味成分的影响[J]. 现代食品科技, 2018(6):188-196.
[19] ANDRES A I, CAVA R, RUIZ J. Monitoring volatile compounds during dry-cured ham ripening by solid-phase micro-extraction coupled to a new direct-extraction device[J]. Journal of Chromatography A, 2002, 963(1):83-88.
[20] 席嘉佩, 詹萍,田洪磊,等. 基于SPME-GC-MS和PCA的不同萃取头对新疆烤羊肉香气成分萃取效果比较[J]. 食品科学,2018, 39(10):240-247.
[21] 白雪, 杨爽,孟鑫. GC-MS结合电子鼻检测微生物脂肪酶对乳品风味的影响[J]. 食品工业科技, 2018, 39(14):209-218.
