食品与发酵工业 >

2019 , Vol. 45 >Issue 18: 254 - 262

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.020946

综述与专题评论

基于核酸适配体检测动物性食品中氯霉素残留的研究进展

  • 王鑫 ,
  • 刘河冰 ,
  • 陶晓奇
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  • 1.西南大学 食品科学学院,重庆,400715;
    2.北京维德维康生物技术有限公司,北京,100095;
    3.重庆市特色食品工程技术研究中心,重庆,400715
王鑫(硕士研究生)和刘河水(博士)为共同第一作者

网络出版日期: 2019-11-06

基金资助

国家自然科学基金面上项目(31672605);重庆市基础研究与前沿探索项目(cstc2018jcyjAX0242);重庆市基础科学与前沿技术研究(cstc2017jcyjAX0313);中国博士后科学基金面上资助(2016M590855);重庆市博士后科研项目特别资助(Xm2017 074)

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Advances in detection of chloramphenicol residues with aptamers in animal-derived foods

  • WANG Xin ,
  • LIU Hebing ,
  • TAO Xiaoqi
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  • 1. College of Food Science, Southwest University, Chongqing 400715, China;
    2. Beijing WDWK Biotech Co., Ltd, Beijing 10095, China;
    3. Chongqing Engineering Research Center of Regional Food, Chongqing 400715, China

Online published: 2019-11-06

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摘要

氯霉素是一种禁用于食品动物的广谱抗生素,建立对其残留的快速检测尤为重要;核酸适配体作为新型生物识别元件,稳定性好且易合成,结合核酸适配体对氯霉素进行残留检测是继传统抗体检测之后又一新的突破方向,因此具有良好发展前景。在此综述了近5年基于核酸适配体检测动物性食品中氯霉素残留的研究进展,主要包括比色分析法、荧光分析法、化学发光分析法、表面增强拉曼散射检测法以及电化学生物传感器法等,发现当前适配体在氯霉素残留检测领域缺乏系统深入研究,相关检测应用难以同时兼顾简单快速和灵敏准确的检测需求,特此比较和讨论不同分析方法,并分析其优缺点及发展趋势,以期为动物性食品中氯霉素残留的高效监管提供有益参考。

关键词： 核酸适配体; 氯霉素; 分析方法; 动物性食品

本文引用格式

王鑫 , 刘河冰 , 陶晓奇 . 基于核酸适配体检测动物性食品中氯霉素残留的研究进展[J]. 食品与发酵工业, 2019 , 45(18) : 254 -262 . DOI: 10.13995/j.cnki.11-1802/ts.020946

Abstract

As one of the broad spectrum antibacterial antibiotics, the rapid detection of chloramphenicol(CAP) is crucial because it was banned from food animals. Aptamer is a novel biological recognized element with good stability and facile synthesis. Developing sensitive, accurate and rapid detection of CAP with aptamers becomes a new breakthrough and has good development prospects compared with traditional antibody detection. Therefore, the main methods in CAP residues detection with aptamers have been reviewed in recent five years, including colorimetric, fluorescence, chemiluminescence, surface-enhanced Raman scattering and electrochemical biosensor. However, lack of systematic in-depth research, related detection applications are difficult to take into account both rapid and accurate detection requirements. It is hereby to compare different analytical methods and, moreover, analyze the advantages, disadvantages and development trends, which provides a useful reference for the efficient regulation of CAP residues in animal-derived foods.

Key words： aptamer; chloramphenicol; analytical methods; animal-derived food

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