In order to provide a theoretical basis for developing and utilizing mulberry leaf alkaloids, the resistance of mulberry leaf alkaloids on DNA oxidative stress in mice was analyzed. A DNA oxidative damage model was induced by D-Gal and different doses of mulberry alkaloids were administered. At the end of the 8th week, the contents of 8-OH-dG and 5-OH-dC, enzyme activities of MTH1, OGG1 and MUTYH in plasma, the liver DNA ladder results and the expressions of MTH1, OGG1 and MUTYH mRNA in liver tissue were detected. Compared with the control, the plasma 8-OH-dG and 5-OH-dC in mice significantly decreased by 65.35% and 59.13%, respectively, after intragastric administration of the highest dose of mulberry leaf alkaloids (200 mg/kg·BW) (P<0.01). Moreover, the activities of MTH1, OGG1 and MUTYH in plasma increased by 37.09%, 134.44% and 114.29%, respectively, and their mRNA levels in liver increased by 192.35%, 103.96% and 109.36%, respectively (P<0.01). In addition, there was no DNA ladder observed in liver in the highest dose mulberry leaf alkaloid group (P>0.05). Therefore, mulberry leaf alkaloids have good repairing effects on D-Gal-induced DNA oxidative damage, and the mechanisms may be due to regulating relevant enzyme activity at transcription level through BER pathway.
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