该研究以鲟鱼皮为原料利用碱性蛋白酶水解制备胶原蛋白肽,进行了鲟鱼皮基本成分分析,酶解条件优化和酶解产物抗氧化活性研究。结果表明,最佳酶解条件为pH 9,温度55℃,添加酶质量分数3%,酶解时间5 h时水解度为22.0%。酶解液经超滤分离得到4种不同分子质量范围的胶原蛋白肽组分SSCP-I(分子质量>10 000 Da)、SSCP-II(分子质量=5 000~10 000 Da)、SSCP-Ⅲ(分子质量=1 000~5 000 Da)和SSCP-IV(分子质量<1 000 Da)。其中(分子质量=1 000~5 000 Da) SSCP-Ⅲ对超氧阴离子自由基的清除能力最高,其半抑制清除浓度IC50为5.938 g/L。该研究为鲟鱼皮的综合加工和高值化利用提供了理论指导。
Collagen peptides are products from collagen degradation, which have antioxidant properties. Collagen peptides were prepared from sturgeon skin by alcalase, followed by analyzing the composition of sturgeon skin, optimizing the enzymatic condition and revealing the antioxidant properties of enzymatic products. The hydrolysis degree reached 22% at the optimal enzymatic hydrolysis condition: hydrolyzed at pH 9 and 55℃ for 5 h with 3% enzyme. Four polypeptides with different molecular weights were obtained by ultrafiltrating the enzymatic hydrolysate, including SSCP-I (MW>10 000 Da), SSCP-II (MW=5 000-10 000 Da), SSCP-III (MW=1 000-5 000 Da) and SSCP-IV (MW<1 000 Da). Among them, SSCP-III had the highest scavenging ability for superoxide anion radicals with an IC50 of 5.938 mg/mL. This research provides a theoretical guidance for comprehensive processing and high value utilization of sturgeon skin.
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