食品与发酵工业 >

2019 , Vol. 45 >Issue 20: 262 - 266

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.020608

分析与检测

聚γ-氨基丁酸修饰电极测定食品中的莱克多巴胺

  • 陈美凤 ,
  • 孙章华 ,
  • 程道娟 ,
  • 赵延慧 ,
  • 马心英
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  • 1(菏泽学院 化学化工学院,山东 菏泽,274015);
    2(山东菏泽市环境监测中心站,山东 菏泽,274000)
硕士研究生,副教授(马心英副教授为通讯作者, E-mail:maxinying5966@163.com)

收稿日期: 2019-03-20

  网络出版日期: 2019-12-20

基金资助

国家自然科学基金项目(21602097);山东省高校科技计划项目(J17KB062);菏泽学院海外智力为国服务行动计划

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Determination of ractopamine in food by poly-γ-aminobutyric acid modified electrode

  • CHEN Meifeng ,
  • SUN Zhanghua ,
  • CHENG Daojuan ,
  • ZHAO Yanhui ,
  • MA Xinying
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  • 1 (College of Chemistry and Chemical Engineering of Heze University, Heze 274015, China);
    2(Environmental Monitoring Central Station of Heze, Heze 274000, China)

Received date: 2019-03-20

  Online published: 2019-12-20

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摘要

采用循环伏安法制备聚γ-氨基丁酸修饰电极,并对聚合条件进行了优化,以建立测定食品中莱克多巴胺含量的电化学分析方法。用循环伏安或差分脉冲法探讨了莱克多巴胺在聚γ-氨基丁酸修饰电极上的电化学行为。结果表明,莱克多巴胺的氧化峰电流与其浓度在6.0×10-8~1.0×10-5 mol/L范围线性关系良好,R2=0.998 8。检出限为8.0×10-9 mol/L。回收率为98.0%~102%,RSD为2.3%~3.1%。循环伏安法简单、快速、灵敏,为莱克多巴胺的测定提供了新方法,为食品控制提供了依据。

关键词： 莱克多巴胺; 修饰电极; 聚γ-氨基丁酸; 循环伏安法; 食品

本文引用格式

陈美凤, 孙章华, 程道娟, 赵延慧, 马心英. 聚γ-氨基丁酸修饰电极测定食品中的莱克多巴胺[J]. 食品与发酵工业, 2019, 45(20): 262-266. DOI: 10.13995/j.cnki.11-1802/ts.020608

Abstract

To establish a method for the determination of ractopamine in food by poly-γ-aminobutyric acid modified electrode, the poly-γ-aminobutyric acid was synthesized on the surface of glassy carbon electrode using an electrochemical method. The polymerization conditions of poly-γ-aminobutyric acid modified electrode were discussed and optimized. Electrochemical behavior of ractopamine was investigated at poly modified electrode by cyclic voltammetry or differential pulse method. A good linear relationship was obtained between the oxidation peak current and concentration of ractopamine in the range of 6.0×10-8-1.0×10-5 mol/L (R=0.998 8), with the detection limit of 8.0×10-9 mol/L. The recoveries ranged from 98.0% to 102%, and RSD was 2.3%-3.1%. This method is simple, rapid and sensitive, which creates a new method for the determination of ractopamine and provides a basis for food control.

Key words： ractopamine; modified electrode; poly-γ-aminobutyric acid; cyclic voltammetry; food

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