采用溶剂萃取和离心分离,将紫色红曲菌W-2发酵液分为上、中、下三相,并用分光光度计在300~600 nm对发酵液及三相中的色素提取液进行扫描。上层相和中层相中的色素是脂溶性的,下层相的是水溶性的。4种色素提取液的扫描图显示出的波峰所对应的波长分别是:发酵液萃取物406和466 nm,上层相391和468 nm,中层相408和468 nm,下层水相332 nm。用板层析法对这三相中的红曲色素分离,总计发现20种以上的红曲色素,大多数是黄色素和橙色素;测定了这些色素的Rf值。比较板层析法和分光光度计法的结果,可看出,前者可以直观地观察到样品中色素的色调、种类及数量,可评估色素相对含量;后者虽然方法简便,但只能用于色素的初步定性(色调)及主要色素相对含量的评判。
The fermentation broth of Monascus purpureus W-2 was separated to three phases by solvent extraction and centrifugation. The pigments in the extracts of the broth and these three phases were scanned using a spectrophotometer at 300-600 nm. It was found that most of these pigments in the upper and middle phase were liposoluble. And the pigments in the lower phase were water-soluble. The wave lengths corresponding to the wave peaks for the pigments in the extracts of the broth were 406 and 466 nm; and were 391 and 468 nm in the upper phase; 408 and 468 nm in the middle phase; and 332 nm in the lower phase. After separation by thin layer chromatography (TLC), more than 20 varieties of pigments were detected in the fermentation broth, and most of them were monascorubrin(orange) and monascin(yellow). The Rf values of these pigments were determined. By TLC method, the color types, quantities of these pigments and their contents can be determined, while the scanning method is simple but can only be used for preliminary qualitative analysis and the evaluation of the relative content of the main pigment.
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