食品与发酵工业 >

2020 , Vol. 46 >Issue 10: 35 - 43

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.023271

研究报告

罗伊氏乳杆菌CCFM8631缓解小鼠非酒精性脂肪性肝病与其对肠道菌群及短链脂肪酸的调节显著相关

  • 焦婷 ,
  • 朱慧越 ,
  • 司倩 ,
  • 许梦舒 ,
  • 孙姗姗 ,
  • 马方励 ,
  • 王刚 ,
  • 赵建新 ,
  • 张灏 ,
  • 陈卫
展开
  • 1(江南大学 食品学院,江苏 无锡,214122)
    2(无限极(中国)有限公司,广东 广州,510623)
硕士研究生(马方励工程师和王刚副教授为共同通讯作者,E-mail:Mary.Ma@infinitus-int.com;wanggang@jiangnan.edu.cn)

收稿日期: 2020-01-06

  网络出版日期: 2020-06-17

基金资助

国家自然科学基金项目(31671839)

收起

Significant correlation between relieving of non-alcoholic fatty liver disease and regulation on gut microbiota and short-chain fatty acids in mice by Lactobacillus reuteri CCFM8631

  • JIAO Ting ,
  • ZHU Huiyue ,
  • SI Qian ,
  • XU Mengshu ,
  • SUN Shanshan ,
  • MA Fangli ,
  • WANG Gang ,
  • ZHAO Jianxin ,
  • ZHANG Hao ,
  • CHEN Wei
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  • 1(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    2(Infinitus (China) Company Ltd., Guangzhou 510623, China)

Received date: 2020-01-06

  Online published: 2020-06-17

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摘要

为探究不同乳酸菌(lactic acid bacteria,LAB)对高脂高胆固醇饮食(high-fat and high-cholesterol diet,HFHCD)诱导的非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)小鼠的疾病进程的影响,该研究对4周龄C57BL/6J小鼠喂食HFHCD的同时灌胃不同LAB 23周。实验结束时,测定小鼠血脂血糖指标、肝脏病理指标、粪便短链脂肪酸(short-chain fatty acids,SCFAs)的含量以及肠道菌群情况。结果表明,补充LAB能够缓解NAFLD小鼠体重增量增加以及肝脏损伤,但在血脂和血糖调控上具有不同效果,其中罗伊氏乳杆菌CCFM8631在各方面均有良好的调控效果,另外,CCFM8631的摄入显著提高了4种SCFAs的含量,上调了Bifidobacterium、Lactobacillus和Desulfovibrio的丰度,降低了Mucispirillum、Turicibacter、SMB53以及Allobaculum的丰度。肠道内指标与肝脏病理指标之间的相关性分析表明,SCFAs水平和肠道菌群结构与NAFLD的缓解显著相关。因此,罗伊氏乳杆菌CCFM8631对肠道菌群结构及SCFAs含量的调节可能是其发挥缓解NAFLD作用的重要途径。该研究为NAFLD预防或治疗提供了新的选择,且为制备防治NAFLD的益生菌制剂提供了参考依据。

关键词： 罗伊氏乳杆菌; 高脂高胆固醇饮食; 非酒精性脂肪性肝病; 短链脂肪酸; 肠道菌群

本文引用格式

焦婷 , 朱慧越 , 司倩 , 许梦舒 , 孙姗姗 , 马方励 , 王刚 , 赵建新 , 张灏 , 陈卫 . 罗伊氏乳杆菌CCFM8631缓解小鼠非酒精性脂肪性肝病与其对肠道菌群及短链脂肪酸的调节显著相关[J]. 食品与发酵工业, 2020 , 46(10) : 35 -43 . DOI: 10.13995/j.cnki.11-1802/ts.023271

Abstract

In order to investigate the effects of different lactic acid bacteria (LAB) on the progression of non-alcoholic fatty liver disease (NAFLD) in mice which was induced by a high-fat and high-cholesterol diet (HFHCD), 4-week-old C57BL/6J mice were fed with HFHCD while administrated different LAB for 23 weeks. At the end of experiment, the blood lipid, blood glucose, liver inflammation, liver damage, fecal short-chain fatty acids (SCFAs), and gut microbiota were evaluated. It was showed that LAB supplementation prevented abnormal weight gain and liver damage induced by HFHCD, while showing different effects on the blood lipid and blood glucose regulation. Among all the strains, Lactobacillus reuteri CCFM8631 showed the highest capacity in alleviation of NAFLD. Furthermore, at the end of the treatment, the fecal SCFAs content and the abundance of Bifidobacterium, Lactobacillus, and Desulfovibrio significantly increased, whilst the abundance of Mucispirillum, Turicibacter, SMB53, and Allobaculum were significantly reduced. Correlation analysis between intestinal and liver pathological indicators showed that regulation on the SCFAs and gut microbiota was significantly associated with NAFLD alleviation. Therefore, the regulation on the SCFAs and gut microbiota by Lactobacillus reuteri CCFM8631 may be an important way for it to relieve NAFLD. This study provides a reference for the preparation of probiotic products in NAFLD prevention. This study provides a new option for the prevention and treatment of NAFLD. Also, it provides a reference for the development of probiotic preparations for NAFLD.

Key words： Lactobacillus reuteri; high-fat and high-cholesterol diet; non-alcoholic fatty liver disease; short chain fatty acids; gut microbiota

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