为研究东海海参性腺丙酮提取物(Am-GE)的功能活性,该文采用MTT法检测了Am-GE对HepG2和Caco-2细胞增殖的影响,分析了Am-GE对HepG2细胞凋亡和周期的影响,利用Gimsa染色法检测了Am-GE对Caco-2细胞成瘤能力的影响,并利用实时荧光定量PCR方法检测Am-GE对小鼠巨噬细胞炎症状因子基因mRNA表达水平的影响。结果显示,Am-GE能显著抑制HepG2和Caco-2细胞的增殖作用,促进HepG2细胞的凋亡和增加G2期细胞比率,减弱Caco-2细胞的成瘤能力;同时Am-GE能显著抑制RAW264.7细胞炎症因子TNF-α、IL-1β、IL-6和iNOS基因转录水平的相对表达量。东海海参性腺丙酮提取物具有较好的抗肿瘤和抗炎症作用。
The present study aims to investigate the functional activities of extraction with acetone form Acaudina molpadioidea gonad (Am-GE) in vitro. The effects of Am-GE on cell proliferation were detected by MTT method in HepG2 and Caco-2 cells. Am-GE-influenced cell apoptosis and cycle were measured by flow cytometry. The inflammatory gene mRNA relative expression levels were determined by quantitative real time PCR in the macrophage of mice. Results showed that Am-GE could significantly inhibited cell proliferation in HepG2 and Caco-2 cells, promoted cell apoptosis and the ratio of G2 stage of HepG2 cells, mitigated the tumorigenic ability in Caco-2 cells. Meanwhile, Am-GE significantly inhibited the inflammatory genes relative expression at transcriptional level, namely TNF-α, IL-1β, IL-6 and iNOS. These indicated that Am-GE exhibited better anti-tumor and anti-inflammatory effects.
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