考察超声波诱变对猴头菇菌丝体和子实体中粗多糖含量及活性的影响。将猴头菇菌株进行不同时间的超声波诱变,各诱变菌株经平板培养初筛和液体发酵培养复筛后,最终得到1株菌丝体生物量最大、粗多糖含量最高的猴头菇变异菌株H-2,然后将原始菌株和H-2菌株进行袋料栽培以获得子实体,并对子实体粗多糖含量进行了比较,最后,比较了原始菌株和H-2菌株子实体粗多糖的抑菌活性。变异菌株H-2菌丝体和子实体的粗多糖含量比原始菌株分别提高了1.2%和2.3%,并且H-2子实体粗多糖对金黄色葡萄球菌有显著抑菌效果。猴头菇菌株经适当时间的超声波诱变后,可以提高其菌丝体和子实体中粗多糖的含量和抑菌活性。
To investigate the effects of ultrasonic mutagenesis on the content and activity of crude polysaccharides in mycelium and sporophore of Hericium erinaceus. The strains of Hericium erinaceus were subjected to ultrasonic mutagenesis at different times. After each mutagenized strain was initial screened by plate culture and then rescreened by liquid fermentation culture, a mutant strain of Hericium erinaceus with the highest biomass and the highest crude polysaccharide content was obtained finally. The original strain and the H-2 strain were then subjected to bag cultivation to obtain sporophores, and the crude polysaccharide content of the sporophores were compared. Finally, the antibacterial activities of the crude polysaccharides in the original strain and H-2 strain sporophores were compared. The crude polysaccharide contents of mycelium and sporophores of mutant strain H-2 were increased by 1.2% and 2.3%, respectively, and the crude polysaccharide of H-2 sporophore had significant antibacterial effect against Staphylococcus aureus. The content and antibacterial activity of crude polysaccharides in mycelium and sporophore can be improved by ultrasonic mutagenesis of Hericium erinaceus strains at an appropriate time.
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