以八月瓜为研究对象,对比分析了八月瓜果皮和种籽的化学组成,同时进一步探讨了总酚、总黄酮、总皂苷含量、抗氧化活性以及对α-葡萄糖苷酶的抑制能力。通过超高效液相色谱-质谱联用仪定性分析,从八月瓜中检出9种活性成分,主要为酚类和皂苷类物质。进一步定量研究发现,八月瓜的总酚(72.8~122.4 mg/g DW)、总黄酮(50.5~54.5 mg/g DW)和总皂苷(85.2~90.9 mg/g DW)含量高,尤其是在种籽提取物中。另外,八月瓜具有较高的清除自由基和抑制α-葡萄糖苷酶活性的能力,其中果皮和种籽提取物对DPPH自由基清除能力的IC50值分别为53.56和47.24 μg/mL,果皮和种籽提取物对α-葡萄糖苷酶的活性抑制率的IC50值分别为14.15和15.54 μg/mL。研究结果为进一步探究八月瓜抑制糖尿病机制研究以及开发相关产品提供数据支持。
The phytochemicals, total phenolics, total flavonoids, total saponins, antioxidant activity, and α-glucosidase inhibitory ability of the peels and seeds of Akebia trifoliata were analysed and compared. The results showed nine key functional compounds belonging to phenols and saponins. The total content of phenolics, flavonoids and saponins were 72.8-122.4 mg/g DW, 50.5-54.5 mg/g DW and 85.2-90.9 mg/g DW, respectively. These contents in seeds were significantly higher than those in peels. In addition, their free radicals scavenging ability and α-glucosidase inhibitory acitivity were investigated: the IC50 values of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging in peel and seed extracts were 53.56 μg/mL and 47.24 μg/mL, respectively. The IC50 values of α-glucosidase activity inhibition in peel and seed extracts were 14.15 μg/mL and 15.54 μg/mL, respectively. The results of this study provided data support for further research and product development of Akebia trifoliata.
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