利用蓝莓花青素对pH值的指示作用制备智能包装膜,以期解决食品保鲜过程中新鲜度的检测难题。以结冷胶、沙蒿胶和蓝莓花青素为主要原料,采用流延法制备智能指示膜,研究膜的色差、机械性能、水溶性、水蒸气透过率以及微观结构等分析其性能。结果表明,红外扫描和电镜分析表明沙蒿胶与结冷胶及蓝莓花青素之间具有良好的相容性;沙蒿胶能够提高结冷胶/沙蒿胶-蓝莓花青素膜的厚度、膜的断裂延伸率、水蒸气透过率,降低膜的抗拉伸强度和水溶性;结冷胶/沙蒿胶复配比8:2(质量比)时,花青素膜的断裂延伸率最大为47.36%,水蒸气透过率为7.643×10-6 g·m/(d·Pa·m2),抗拉强度22.08 MPa,复配比4:6(质量比)的膜厚度达到0.079 mm;复合膜对pH值的指示作用显著,可作为pH指示膜。本研究为新型食品保鲜智能指示膜的应用提供理论基础。
A new sensor film of blueberry anthocyanin, which could indicate pH value changes, was prepared for food freshness detection in the process of food preservation. The intelligent packaging film of gellan gum/Artemisia sphaerocephala Krasch gum-blueberry anthocyanin was prepared by casting method. The color difference, mechanical properties, water solubility, water vapor permeability and microstructure of the film were investigated. The results of Fourier infrared spectroscopy scanning (FTIR) and scanning electron microscopy (SEM) showed that there was good compatibility between Artemisia sphaerocephala Krasch gum with gellan gum and blueberry anthocyanin. The thickness, elongation at break (EAB) and water vapor permeability (WVP) of film enhanced with addition of Artemisia sphaerocephala Krasch gum, while tensile strength (TS) and water solubility of the film reduced. EAB of the anthocyanin film with a compounding ratio of 8:2 reached 47.36%, WVP 7.643×10-6 g·m/(d·Pa·m2), TS at 22.08 MPa. The film thickness of film (4:6) reached 0.079 mm respectively. Furthermore, there were significant color response of the film on the pH conditions and the colors of the film displayed from red to purple as pH condition changed. Therefore, the gellan gum/Artemisia sphaerocephala Krasch gum-blueberry anthocyanin edible film could be used as a novel food preservation pH intelligent indicator film, and these results indicate a theoretical basis of intelligent indicator film for food preservation.
[1] LIU J, WANG H, WANG P, et al. Films based on κ-carrageenan incorporated with curcumin for freshness monitoring [J]. Food Hydrocolloids, 2018, 83: 134-142.
[2] CHOI I, LEE J Y, LACROIX M, et al. Intelligent pH indicator film composed of agar/potato starch and anthocyanin extracts from purple sweet potato [J]. Food Chemistry, 2017, 218: 122-128.
[3] TIAN X Y, CAI Q, ZHANG Y M. Rapid classification of hairtail fish and pork freshness using an electronic nose based on the PCA method[J]. Sensors, 2012, 12(1): 260-277.
[4] LAWAL A T, ADELOJU S B. Progress and recent advances in fabrication and utilization of hypoxanthine biosensors for meat and fish quality assessment: a review[J]. Talanta, 2012, 100: 217-228.
[5] 蒋光阳,肖力源,王章英,等.不同基材复配紫薯花青素制备智能指示膜及其应用[J].食品科学,2019, 40(13):267-273.
[6] ZHAI X, SHI J, ZOU X, et al. Novel colorimetric films based on starch/polyvinyl alcohol incorporated with roselle anthocyanins for fish freshness monitoring[J]. Food Hydrocolloids, 2017, 69: 308-317.
[7] CABRITA L, FOSSEN T, ANDERSEN M. Colour and stability of the six common anthocyanidin 3-glucosides in aqueous solutions[J]. Food Chemistry, 2000, 68(1): 101-107.
[8] SUI X. Changes in the color, chemical stability and antioxidant capacity of thermally treated anthocyanin aqueous solution over storage[M].Impact of Food Processing on Anthocyanins. Springer, Singapore, 2017: 49-65.
[9] LU M, LI Z, LIANG H, et al. Controlled release of anthocyanins from oxidized konjac glucomannan microspheres stabilized by chitosan oligosaccharides[J]. Food Hydrocolloids, 2015, 51: 476-485.
[10] LIANG T, SUN G, CAO L, et al. A pH and NH3 sensing intelligent film based on Artemisia sphaerocephala Krasch. gum and red cabbage anthocyanins anchored by carboxymethyl cellulose sodium added as a host complex[J]. Food Hydrocolloids, 2019, 87: 858-868.
[11] ZIA K M, TABASUM S, KHAN M F, et al. Recent trends on gellan gum blends with natural and synthetic polymers: a review[J]. International Journal of Biological Macromolecules, 2018, 109: 1 068-1 087.
[12] ZHANG N, XU J, GAO X, et al. Factors affecting water resistance of alginate/gellan blend films on paper cups for hot drinks[J]. Carbohydrate Polymers, 2017, 156: 435-442.
[13] ZHU G, SHENG L, TONG Q. Preparation and characterization of carboxymethyl-gellan and pullulan blend films[J]. Food Hydrocolloids, 2014, 35: 341-347.
[14] 朱桂兰, 陶思远,童群义. 结冷胶与黄原胶复配体系流变与凝胶特性[J]. 食品与发酵工业, 2013, 39(3): 56-60.
[15] MEI T, XU X, LI B, et al. Synergistic interaction of konjac glucomannan and gellan gum investigated by rheology and texture analysis[J]. Journal of Applied Polymer Science, 2012, 125(2): 1 363-1 370.
[16] 刘敦华, 谷文英. 沙蒿籽胶的流变学性质研究[J]. 食品科学, 2006,27(2): 76-80.
[17] 刘敦华, 谷文英,丁霄霖. 沙蒿胶对面团流变性质的影响及在面包加工中的应用[J]. 农业工程学报, 2009, 25(S1): 233-236.
[18] 谌小立,黄先静,杨旭芹,等. 三种食用油对红酸汤烹调中全反式番茄红素含量影响研究[J].中国调味品, 2019, 44(3): 91-94.
[19] 陈文莹, 杨旭芹,刘臻,等. 烹调三种蔬菜对红酸汤中全反式番茄红素含量的影响[J]. 中国调味品, 2019, 44(7): 1-4.
[20] WEI Y C, CHENG C H, HO Y C, et al. Active gellan gum/purple sweet potato composite films capable of monitoring pH variations[J]. Food Hydrocolloids, 2017, 69: 491-502.
[21] LUCHESE C L, GARRIDO T, SPADA J C, et al. Development and characterization of cassava starch films incorporated with blueberry pomace[J]. International Journal of Biological Macromolecules, 2018, 106: 834-839.
