食品与发酵工业 >

2020 , Vol. 46 >Issue 5: 1 - 8

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.023280

研究报告

唾液乳杆菌CCFM 1054通过改变肠道菌群缓解空肠弯曲杆菌在小鼠体内的感染

  • 金星 ,
  • 贺禹丰 ,
  • 周永华 ,
  • 陈晓华 ,
  • 王刚 ,
  • 赵建新 ,
  • 张灏 ,
  • 陈卫
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  • 1(江南大学 食品学院,江苏 无锡,214122)
    2(国家卫生健康委寄生虫病预防与控制技术重点实验室,江苏 无锡,214064)
    3(衡阳师范学院 生命科学与环境学院,湖南 衡阳,421008)
博士研究生(周永华研究员和王刚副教授为共同通讯作者,E-mail:zhouyonghua@jipd.com;wanggang@jiangnan.edu.cn)

收稿日期: 2020-01-07

  网络出版日期: 2020-04-10

基金资助

国家自然科学基金资助项目(31671839,31601444,31301407);湖南省教育厅资助科研项目(15B034);湖南省自然科学基金资助项目(2019JJ50014)

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Lactobacillus salivarius CCFM 1054 alleviates the infection ofCampylobacter jejuni in mice by regulating the gut microbiota

  • JIN Xing ,
  • HE Yufeng ,
  • ZHOU Yonghua ,
  • CHEN Xiaohua ,
  • WANG Gang ,
  • ZHAO Jianxin ,
  • ZHANG Hao ,
  • CHEN Wei
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  • 1(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    2(Key Laboratory of Parasitic Disease Prevention and Control Technology for National Health andHealth Commission,Wuxi 214064, China)
    3(College of Life Science and Environment, Hengyang Normal University, Hengyang 421008, China)

Received date: 2020-01-07

  Online published: 2020-04-10

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摘要

考察了唾液乳杆菌(Lactobacillus salivarius)CCFM 1054体外培养产酸及发酵上清液抑制空肠弯曲杆菌(Campylobacter jejuni)生长能力、对人工模拟胃肠液中的耐受、对共培养条件下的抑菌能力、对HT-29细胞的粘附以及自我形成生物膜的能力,并以鼠李糖乳杆菌LGG和植物乳杆菌N49作为对比菌株,分别干预空肠弯曲杆菌和弓形虫复合感染的小鼠。结果显示,CCFM 1054能显著改变小鼠肠道菌群的组成,降低空肠弯曲杆菌在小鼠体内的定植率并缓解其感染。肠道菌群变化和乳酸菌拮抗空肠弯曲杆菌相关的体内外特性的相关性分析表明,CCFM 1054对细胞的高粘附性及其较强的生物膜形成能力使得其能在小鼠体内显著改变肠道菌群丰度。

关键词： 唾液乳杆菌; 空肠弯曲杆菌; 肠道菌群

本文引用格式

金星 , 贺禹丰 , 周永华 , 陈晓华 , 王刚 , 赵建新 , 张灏 , 陈卫 . 唾液乳杆菌CCFM 1054通过改变肠道菌群缓解空肠弯曲杆菌在小鼠体内的感染[J]. 食品与发酵工业, 2020 , 46(5) : 1 -8 . DOI: 10.13995/j.cnki.11-1802/ts.023280

Abstract

In this study, the biological characteristics of Lactobacillus salivarius CCFM 1054 in vitro were evaluated, such as the ability of acid production, the antibacterial ability of free supernatant for Campylobacter jejuni growth, the tolerance to artificial gastrointestinal fluids, the inhibition to co-cultured C. jejuni, the adhesion to HT-29 cell and the ability of biofilms generation. It was founded that CCFM 1054 had strong acid-producing ability and could inhibit the growth of C. jejuni in vitro. At the same time, it had good tolerance under artificial simulated gastrointestinal fluid, high adhesion to HT-29 cells and strong self-film-forming ability. The L. rhamnosus LGG and L. plantarum N49 were used as control strains to CCFM 1054 when administered to C. jejuni and Toxoplasma co-infected mice by gavage. The results showed that CCFM 1054 could significantly change the composition of gut microbiota, such as Firmicutes, Bacteroidetes and Proteobacteria at the phylum level and Campylobacter, Lactobacillus, Pediococcus, Faecoccus, Coprococcus and Unclassified Enterobacteriaceae at the genera level. Changes in gut microbes reduced the colonization of C. jejuni in mice and alleviated the infection of C. jejuni in vivo. The correlation between the changes of gut microbiota and the biological characteristics of lactic acid bacteria in vitro indicated that CCFM 1054's high adhesion to HT-29 cell and strong biofilm-forming ability made it change the gut microbiota in mice significantly.

Key words： Lactobacillus salivarius; Campylobacter jejuni; gut microbiota

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