为开发brevilaterin的高效提取技术,首先,通过考察大孔树脂对brevilaterin的吸附解吸附能力,筛选出吸附解吸附效果好的大孔树脂XAD-7HP,并通过优化获得最佳工艺条件,即将大孔树脂XAD-7HP以30 g/L的添加量于35 ℃条件下吸附发酵液中的brevilaterin,吸附时间为1.5 h,再采用70%乙醇解吸附2 h,brevilaterin回收率达到82%。通过吸附等温线模型及吸附动力学研究了XAD-7HP对brevilaterin的吸附机理,发现XAD-7HP对brevilaterin的吸附符合Langmuir模型与拟二级动力学,说明XAD-7HP吸附brevilaterin的过程属于单分子层的化学吸附;Elovich方程拟合结果显示,吸附过程主要分为3个阶段:边界层扩散、颗粒内扩散、最终平衡。大孔树脂法提取brevilaterin工艺绿色环保、操作简单、成本低廉,为brevilaterin的工业化生产提供了技术支持。
The antimicrobial peptide brevilaterin from Brevibacillus laterosporus has the advantages of high safety, broad antibacterial spectrum and good thermal stability. However, the extraction process of brevilaterin was tedious, which resulted in the high production cost and difficulty in large scale production. Hence, here we aimed to develop an efficient extraction technology of brevialterin. First, the macroporous resins XAD-7HP was screened from eight resins by evaluating the adsorption-desorption ability, and then the adsorption-desorption condition was optimized. The recovery rate reached 82% under the optimal conditions: addition of 30 g/L XAD-7HP, adsorption at 35 ℃ for 1.5 h, then adding 70% ethanol and desorption for 2 h. Then, the adsorption mechanism was investigated by adsorption isotherms and thermodynamics. The results showed that the adsorption of brevilaterin by XAD-7HP fitted Langmuir model and pseudo-second-order kinetics, suggesting that it belongs to chemical adsorption of monolayer. Besides, the results on Elovich models demonstrated that the adsorption process was mainly divided into three stages: boundary layer diffusion, particle diffusion and final equilibrium. In conclusion, the extraction process of brevilaterin by macroporous resin was green and environmentally friendly, easy to operation, and cost-saving, which provids technical support for the industrial production of brevilaterin.
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