为开拓富硒食品新途径,得到具有较强富硒能力的乳酸菌。以不同浓度的亚硒酸钠作用于5种乳酸菌,通过观察菌体情况,结合菌落数筛选出1株富硒优势乳酸菌。采用单因素实验结合响应面法对富硒条件进行优化,并对富硒优化后的乳酸菌活性进行评价。筛选出植物乳杆菌为富硒优势菌,优化后最佳富硒条件为NaCl质量浓度为2 g/100mL、亚硒酸钠质量浓度为16 μg/mL、接种量为3%,富硒率为84.26%。扫描电镜分析富硒植物乳杆菌菌体未出现变形,且经富集后表面有少量单质硒析出。在模拟胃肠道消化中富硒植物乳杆菌经消化后活菌数均在7 lgCFU/mL以上,存活率显著高于未富硒组(P＜0.05)。说明在此富硒培养条件下植物乳杆菌能发挥更好的功能性和益生性,为后续研究富硒乳酸菌发酵食品提供实验数据支撑。
易鑫
,
周琦
,
欧阳祝
,
谈安群
,
范佳莹
,
李则灵
,
朱霞建
,
黄林华
,
李贵杰
,
王华
. 乳酸菌富硒优化及其活性评价[J]. 食品与发酵工业, 2020
, 46(8)
: 179
-186
.
DOI: 10.13995/j.cnki.11-1802/ts.023276
In order to explore a new approach for selenium-rich food and preparation of selenium-enriched probiotics, five Lactobacillus strains were treated with different concentrations of sodium selenite. One Lactobacillus strain was screened by observing the bacteria condition and the colony number. The conditions of selenium enrichment were optimized by single factor experiment and response surface method, the activity of lactic acid bacteria was evaluated after selenium enrichment optimization. The Lactobacillus plantarum was screened as the selenium-rich dominant bacteria. The optimal conditions for selenium enrichment were as follows: NaCl 2 g/100mL, sodium selenite 16 μg/mL, inoculation amount 3%, and selenium enrichment rate 84.26%. Scanning electron microscopy (SEM) showed that the selenium-rich L. plantarum didn’t deform, and selenium was generated on the surface after enrichment. After digestion in simulated gastrointestinal, the number of viable bacteria of se-enriched L. plantarum was above 7 lgCFU/mL, and the survival rate was significantly higher than that of the non-selenium-enriched group (P <0.05), and could play a better functional and probiotic role, providing experimental data support for the subsequent study of selenium-rich lactic acid bacteria fermentation food.
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