该研究旨在建立超快速液相色谱-质谱联用技术(ultra-fast liquid chromatography-mass spectrometry,UFLC-MS/MS)分析方法,对薄荷中31种氨基酸和核苷类成分进行分析评价,为薄荷的资源综合利用提供科学依据。应用Waters XBridge Amide 色谱柱(100 mm×2.1 mm,3.5 μm);流动相为0.2%甲酸水-0.2%甲酸乙腈,梯度洗脱,流速0.2 mL/min,柱温30 ℃,正离子多反应监测(multiple reaction monitoring,MRM)模式测定。采用PCA和TOPSIS方法对样品进行综合评价。结果表明,21种氨基酸和10种核苷类成分线性关系良好(r2>0.999 0),方法精密度、重复性、稳定性良好,RSD值均大于3.85%,加样回收率在94.18%～98.76%,RSD在1.17%～3.56%。薄荷中含有丰富的氨基酸和核苷类成分,不同产地间含量差异较大,其中以阜阳产薄荷样品综合质量最优。该方法准确、高效,结果可为药食两用薄荷的质量评价和综合利用提供可靠依据。
The aim of this study was to establish an ultra-fast liquid chromatography-mass spectrometry (UFLC-MS/MS) method for the analysis and evaluation of 31 amino acids and nucleosides in peppermint in order to provide scientific knowledge for the comprehensive utilization of mint resources. The UFLC-MS/MS parameters were as follows: Waters XBridge Amide column (100 mm×2.1 mm, 3.5 μm); mobile phase 0.2% formic acid -0.2% formic acid acetonitrile, gradient elution, flow rate 0.2 mL/min, column temperature 30 ℃, positive Ion multiple reaction monitoring (MRM) mode determination. PCA and TOPSIS methods were used for the comprehensive evaluation of the samples. The 21 amino acids and 10 nucleosides showed a good linear relationship with the peak area within a certain concentration range (r2>0.999 0). The precision, repeatability and stability of the method were good, and the RSD values were all higher than 3.85%. The recovery rate of the sample was between 94.18% and 98.76% and the RSD was between 1.17% and 3.56% respectively. Peppermint is rich in amino acids and nucleosides and the content varies significantly from different regions. The quality of peppermint samples from Fuyang was the best. In summary, this method developed here is accurate and efficient and the results could provide a reliable basis for the quality evaluation and comprehensive utilization of the dual-use mint.
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