该文建立了一种高效液相色谱二极管阵列检测器同时测定蓝莓酒中6种花青素(飞燕草色素、矢车菊色素、矮牵牛色素、天竺葵色素、芍药素、锦葵色素)质量浓度的方法。样品采用 V(乙醇)∶V(水)∶V(盐酸)=2∶1∶1溶液超声提取,120 ℃ 的烘箱中加热水解后测定。以0.1%的磷酸-乙腈溶液作为流动相,梯度洗脱,经Poroshell 120 EC-C₁₈柱分离,检测波长为525 nm。结果表明,6种花青素的浓度与峰面积在0.5～10 mg/L的质量浓度范围内具有良好线性关系,相关系数r>0.999 9,6种花青素的回收率为80.50%~108.80%(相对标准偏差为0.54%~5.63%),除了锦葵色素的检出限为0.10 mg/kg外,其余5种花青素的检出限均为0.15 mg/kg。该方法简便快捷、精确可靠、重现性好,适用于蓝莓酒中6种花青素的检测。

A simple, accurate and stable method for the analysis of anthocyanins in blueberry wine was established. Six kinds of anthocyanins, namely, delphinidin, cyanidin, petunidin, pelargonidin, peonidin, and malvidin were determined by high-performance liquid chromatography with diode array detection. A mixture of ethanol, water and hydrochloric acid at a ratio of 2∶1∶1(V/V/V) was used for the ultrasonic extraction. The samples were detected after heating and hydrolysis in an oven at 120 ℃. Poreshell 120 EC-C₁₈ column was used as an analytical column. A mixture of 0.1% phosphoric acid and acetonitrile was used as the mobile phase in gradient mode and the detection wavelength was 525 nm. The results showed that the concentration of anthocyanins was in accordance with linear regression in the range of 0.5-10 mg/L (r>0.999 9). The range of the spiked recoveries was 80.50%-108.80%, with the relative standard deviations (RSD) of 0.54%-5.63%. The limit of detection (LOD) obtained for malvidin and other five anthocyanins were 0.10 mg/kg and 0.15mg/kg, respectively. Method validation showed that the developed method was reliable for the analysis of anthocyanins.