茶多酚在抗癌方面具有多种生物和药理作用,但在空气中很容易被氧化,故长期作用受到限制。微胶囊化封装技术可以帮助克服这些缺点。肿瘤血管内栓塞疗法通过微胶囊栓塞剂,阻塞肿瘤细胞增殖需要的养分以及氧气,抑制肿瘤生长。同时,通过磁性微胶囊的药物靶向缓释技术,向癌靶区释放抗癌药物,杀伤癌细胞,毒副作用小,治疗效果显著,是一种治疗癌症的新方法。磁性微胶囊的性能,如包覆率和粒径,受到多种因素的影响。利用Box-Behnken响应曲面的实验设计优化了磁性微胶囊的配方:海藻酸钠质量浓度18.36 g/L,电压6.222 kV,推进速度80 mm/h。在此条件下,磁性微胶囊的包覆率为87.836 2%,平均尺寸为217.67 μm。茶多酚缓释持续20 h以上,有较好的缓释性能。功能性验证显示,优化后的磁性微胶囊相比于优化前,具有更好的缓释性和抗氧化性。
Tea polyphenols exhibited various biological and pharmacological effects on cancer prevention. However, they were easily oxidized in the air, which restricted its long-term effects. Encapsulation technology was one of the most effective methods to overcome these drawbacks. Tumor endovascular embolization therapy could block the nutrients and oxygen for tumor cell proliferation and inhibited tumor growth through microcapsule embolization. Meanwhile, the magnetic microcapsule released anti-cancer drugs into the target area to kill cancer cells. Due to little side effects and remarkable therapeutic effects, this therapy was regarded as a new method to treat cancer. The properties of magnetic microcapsules, such as encapsulation efficiency and particle size, were affected by many factors. This study optimized the formula of tea polyphenol magnetic microcapsules by using the experimental design of BBD-RSM. The optimal formula for the preparation of magnetic microcapsules was as follows: the concentration of sodium carboxymethyl cellulose was 1.863 9%, the voltage was 6.222 kV and the propulsion speed was 80 mm/h, respectively. The encapsulation efficiency of magnetic microcapsules was 87.836 2% and the average particle size was 217.67 μm. The magnetic microcapsules had good antioxidant property, and the release of the magnetic microcapsules was sustained for more than 20 h. The result of functional verification showed that the optimized magnetic microcapsules had better slow-release performance and anti-oxidation property.
[1] YANG G. Inhibition of growth and induction of apoptosis in human cancer cell lines by tea polyphenols[J]. Carcinogenesis, 1998, 19(4):611-616.
[2] 刘学铭, 梁世中. 茶多酚的保健和药理作用及应用前景[J]. 食品与发酵工业, 1998(5):47-51.
[3] 马小明. 微胶囊技术及其在食品工业中的应用[J]. 食品与发酵工业, 1991(5):69-73.
[4] AUGST A D, KONG H J, MOONEY D J. Alginate Hydrogels as Biomaterials[J]. Macromolecular Bioscience, 2006, 6(8):623-633.
[5] GEORGE M, ABRAHAM T E. Polyionic hydrocolloids for the intestinal delivery of protein drugs: Alginate and chitosan-a review[J]. Journal of Controlled Release Official Journal of the Controlled Release Society, 2006, 114(1):1-14.
[6] LÓPEZ CÓRDOBA, ALEX, DELADINO L, MARTINO M. Effect of starch filler on calcium-alginate hydrogels loaded with yerba mate antioxidants[J]. Carbohydrate Polymers, 2013, 95(1):315-323.
[7] 王咏梅, 龚长源, 程永德. 海藻酸钠微球血管栓塞剂治疗子宫
肌瘤二例[J]. 介入放射学杂志, 2004, 13(4):290-290.
[8] 于波涛, 张志荣, 曾仁杰,等. 肝靶向氟尿嘧啶类脂纳米粒的研究[J]. 药学学报, 2000, 35(9):700-705.
[9] DRAGET K I, KJERSTI STEINSVAG, EDVAR ONSOYEN, et al. Na- and K-alginate; effect on Ca2+-gelation[J]. Carbohydrate Polymers, 1998, 35(s 1-2):1-6.
[10] KRASAEKOOPT W, BHANDARI B, DEETH H. The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria[J]. International Dairy Journal, 2004, 14(8):737-743.
[11] 张武杰, 李保国, 张超, 等. 静电喷雾法制备海藻酸钙-羧甲基纤维素钠液芯微胶囊的工艺选优[J]. 上海理工大学学报, 2007, 29(4):349-352.
[12] 李琳琳, 陈东, 丁明慧,等. 磁性微胶囊的制备及其药物缓控释性能[J]. 物理化学学报, 2007, 23(12):1 969-1 973.
[13] FOURNIER C, MICHÈLE HAMON, HAMON M, et al. Preparation and preclinical evaluation of bioresorbable hydroxyethylstarch microspheres for transient arterial embolization[J]. International Journal of Pharmaceutics, 1994, 106(1):41-49.
[14] 方敏, 宫智勇, 王耀峰. 玉米须乙醇提取物体外抗氧化活性研究[J]. 中国食物与营养, 2008(4):45-47.
[15] 旷英姿, 马全红, 郝小祯, 等. 茶多酚脂质体的制备研究[J]. 中国生化药物杂志, 2006, 27(1):28-31.
[16] HARRIS R, LECUMBERRI E, MATEOS-APARICIO I, et al. Chitosan nanoparticles and microspheres for the encapsulation of natural antioxidants extracted from Ilex paraguariensis[J]. Carbohydrate Polymers, 2011, 84(2):803-806.
[17] 高宝玉, 岳钦艳, 于慧,等. 利用透射电镜研究PACS的形态及絮凝机理[J]. 环境科学学报, 1998, 18(4):392-395.
[18] 李向东, 李娟. 粒径分析法研究稳定剂对含乳饮料稳定性的影响[J]. 食品科学, 2011, 32(13):58-61.
[19] 吴晓青, 魏俊富, 李嘉禄. 差示扫描量热法研究改性双马来酰亚胺的固化过程[J]. 复合材料学报, 1999, 16(2):34-38.
[20] 丁恩勇, 梁学海. 不同实验条件对DSC峰形的影响以及相变温度的确定[J]. 分析测试学报, 1993(5):50-54.
[21] 宋春财, 胡浩权, 朱盛维,等. 生物质秸秆热重分析及几种动力学模型结果比较[J]. 燃料化学学报, 2003, 31(4):311-316.
[22] 王春红, 李洪昌, 李锡伯, 等. 一种氯化钙交联的海藻酸钠纳米纤维支架材料及其制备方法:, CN105457094A[P].
[23] 李琳琳, 陈东, 丁明慧,等. 磁性微胶囊的制备及其药物缓控释性能[J]. 物理化学学报, 2007, 23(12):1 969-1 973.
[24] 刘军海, 黄宝旭, 蒋德超. 响应面分析法优化艾叶多糖提取工艺研究[J]. 食品科学, 2009, 30(2):114-118.
[25] 刘洋, 赵谋明, 杨宁,等. 响应面分析法优化仙人掌多糖提取工艺的研究[J]. 食品与机械, 2006, 22(6):42-44.
[26] 程琮, 范华. Levene方差齐性检验[J]. 中国卫生统计, 2005, 22(6):408;420.
[27] 范琳琳, 王红瑞, 宋乃琦, 等. 基于T检验的水文时间序列HHT分析方法及应用[J]. 系统工程理论与实践, 2015,35(5):1 324-1 331.
[29] 李志勇. 改性海藻酸钠凝胶的制备及性能研究[D]. 无锡:江南大学, 2008.
