综述与专题评论

基于磁性材料的适配体传感器在赭曲霉毒素超灵敏检测中的研究进展

  • 蔡小霞 ,
  • 苑静 ,
  • 林童 ,
  • 郭徐静 ,
  • 熊勇华 ,
  • 吴科盛 ,
  • 郭亮
展开
  • 1(南昌大学 食品科学与技术国家重点实验室,江西 南昌,330047)
    2(南昌大学 中德联合研究院,江西 南昌,330047)
    3(江西省兽药饲料监察所,江西 南昌,330029)
硕士研究生(郭亮助理研究员为通讯作者,E-mail:bioguo@163.com)

收稿日期: 2020-01-21

  网络出版日期: 2020-06-24

基金资助

“十三五”国家重点研发计划重点专项(2018YFC1602500);国家自然科学基金地区科学基金项目(31360385);江西省教育厅落地计划(KJLD13011)

The progress of aptasensor based on magnetic material in ultratracedetection of ochratoxin

  • CAI Xiaoxia ,
  • YUAN Jing ,
  • LIN Tong ,
  • Guo Xujing ,
  • XIONG Yonghua ,
  • WU Kesheng ,
  • GUO Liang
Expand
  • 1(State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)
    2(Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China)
    3(Jiangxi Province Institute of Veterinary Drug and Feed Control, Nanchang, 330029, China)

Received date: 2020-01-21

  Online published: 2020-06-24

摘要

赭曲霉毒素A(ochratoxin A,OTA)毒性大、分布广,因此建立简便且适于复杂基质的超灵敏OTA检测方法十分必要。基于磁性材料的适配体传感器兼具适配体稳定性高和磁性材料磁操作简便的特点,可实现待测物或检测探针的快速分离富集,有效提高检测灵敏度并缩短检测时间,适于食品中痕量OTA的检测。该文按输出信号类型的不同综述了近年来基于磁性材料的OTA适配体传感器的研究进展。着重阐述了磁性材料、新型纳米材料和生物技术在放大检测信号、提高磁性适配体传感器检测灵敏度中的应用及其机制,旨在为适配体传感器的设计和真菌毒素的痕量检测提供参考依据。

本文引用格式

蔡小霞 , 苑静 , 林童 , 郭徐静 , 熊勇华 , 吴科盛 , 郭亮 . 基于磁性材料的适配体传感器在赭曲霉毒素超灵敏检测中的研究进展[J]. 食品与发酵工业, 2020 , 46(11) : 307 -314 . DOI: 10.13995/j.cnki.11-1802/ts.023443

Abstract

Due to the high toxicity and wide distribution of (ochratoxin A,OTA), it's essential to establish facile and ultrasensitive methods for the detection of OTA in complex matrices. Benefiting from the high stability of aptamer and easy magnetic handling of magnetic material, the magnetic material-based aptasensor could achieve a rapid magnetic separation and enrichment of analytes or detection probes to improve the detection sensitivity and shorten the detection duration effectively which made it extremely suitable for the detection of trace OTA in a food sample. Herein, the recent progress in magnetic material-based OTA aptasensor using different output signals was summarized, with special emphasis on the applications of magnetic materials, novel nanomaterials and biotechnology, as well as their mechanisms in amplifying detection signals and improving the detection sensitivities, aiming at providing a reference for the design of aptasensor and ultratrace detection of mycotoxin.

参考文献

[1] O′BRIEN E, DIETRICH D R. Ochratoxin A: the continuing enigma[J]. Critical Reviews in Toxicology, 2005, 35(1): 33-60.
[2] 周景明, 李春革, 祁艳华, 等. 赭曲霉毒素 A 完全抗原的制备及鉴定[J]. 动物医学进展, 2016, 37(1): 38-42.
[3] 黎睿, 谢刚, 王松雪. 高效液相色谱法同时检测粮食中常见 8 种真菌毒素的含量[J]. 食品科学, 2015,36(6): 206-210.
[4] LIU B H, TSAO Z J, WANG J J, et al. Development of a monoclonal antibody against ochratoxin A and its application in enzyme-linked immunosorbent assay and gold nanoparticle immunochromatographic strip[J]. Analytical Chemistry, 2008, 80(18): 7 029-7 035.
[5] CRUZ-AGUADO J A, PENNER G. Determination of ochratoxin A with a DNA aptamer[J]. Journal of Agricultural and Food Chemistry, 2008, 56(22): 10 456-10 461.
[6] AGUILAR-ARTEAGA K, RODRIGUEZ J A, BARRADO E. Magnetic solids in analytical chemistry: a review[J]. Analytica Chimica Acta, 2010, 674(2): 157-165.
[7] SU Y, SHAO C G, HUANG X L, et al. Extraction and detection of bisphenol A in human serum and urine by aptamer-functionalized magnetic nanoparticles[J]. Analytical and Bioanalytical Chemistry, 2018, 410(7): 1 885-1 891.
[8] CHEN J, HAO L, WU Y, et al. Integrated magneto-fluorescence nanobeads for ultrasensitive glycoprotein detection using antibody coupled boronate-affinity recognition[J]. Chemical Communications, 2019, 55(69): 10 312-10 315.
[9] XIANYU Y L, WANG Q L, CHEN Y P. Magnetic particles-enabled biosensors for point-of-care testing[J]. TrAC Trends in Analytical Chemistry, 2018, 106: 213-224.
[10] SELIM Ü, CHIARI M, ÖZCAN A. Introduction to the special issue of optical biosensors[J]. Nanophotonics, 2017, 6(4): 623-625.
[11] LIN C, ZHENG H, SUN M, et al. Highly sensitive colorimetric aptasensor for ochratoxin A detection based on enzyme-encapsulated liposome[J]. Analytica Chimica Acta, 2018, 1 002: 90-96.
[12] HUANG Y, REN J, QU X. Nanozymes: classification, catalytic mechanisms, activity regulation, and applications[J]. Chemical Reviews, 2019, 119(6): 4 357-4 412.
[13] WANG C, QIAN J, WANG K, et al. Colorimetric aptasensing of ochratoxin A using Au@ Fe3O4 nanoparticles as signal indicator and magnetic separator[J]. Biosensors and Bioelectronics, 2016, 77: 1 183-1 191.
[14] TIAN F, ZHOU J, JIAO B, et al. A nanozyme-based cascade colorimetric aptasensor for amplified detection of ochratoxin A[J]. Nanoscale, 2019,11(19):9 547-9 555.
[15] HAO N, LU J, ZHOU Z, et al. A pH-resolved colorimetric biosensor for simultaneous multiple target detection[J]. ACS Sensors, 2018, 3(10): 2 159-2 165.
[16] 李素, 肖义陂, 武乐, 等. 金标记羟胺放大化学发光检测赭曲霉毒素A[J]. 分析测试学报, 2018,37(1): 57-61.
[17] HUN X, LIU F, MEI Z H, et al. Signal amplified strategy based on target-induced strand release coupling cleavage of nicking endonuclease for the ultrasensitive detection of ochratoxin A[J]. Biosensors and Bioelectronics, 2013, 39(1): 145-151.
[18] ZHANG Z, XIA X, XIANG X, et al. Conjugated cationic polymer-assisted amplified fluorescent biosensor for protein detection via terminal protection of small molecule-linked DNA and graphene oxide[J]. Sensors and Actuators B: Chemical, 2017, 249: 8-13.
[19] LIU Y, YAN H, SHANGGUAN J, et al. A fluorometric aptamer-based assay for ochratoxin A using magnetic separation and a cationic conjugated fluorescent polymer[J]. Microchimica Acta, 2018, 185(9): 1-7.
[20] HAYAT A, MISHRA R K, CATANANTE G, et al. Development of an aptasensor based on a fluorescent particles-modified aptamer for ochratoxin A detection[J]. Analytical and Bioanalytical Chemistry, 2015, 407(25): 7 815-7 822.
[21] WANG R, XIANG Y, ZHOU X, et al. A reusable aptamer-based evanescent wave all-fiber biosensor for highly sensitive detection of Ochratoxin A[J]. Biosensors and Bioelectronics, 2015, 66: 11-18.
[22] DUAN H, HUANG X L, SHAO Y N, et al. Size-dependent immunochromatographic assay with quantum dot nanobeads for sensitive and quantitative detection of ochratoxin A in corn[J]. Analytical Chemistry, 2017, 89(13): 7 062-7 068.
[23] GUO L, SHAO Y, DUAN H, et al. Magnetic quantum dot nanobead-based fluorescent immunochromatographic assay for the highly sensitive detection of aflatoxin B1 in dark soy sauce[J]. Analytical chemistry, 2019, 91(7): 4 727-4 734.
[24] WANG C, QIAN J, WANG K, et al. Magnetic-fluorescent-targeting multifunctional aptasensorfor highly sensitive and one-step rapid detection of ochratoxin A[J]. Biosensors and Bioelectronics, 2015, 68: 783-790.
[25] QIAN J, REN C, WANG C, et al. Magnetically controlled fluorescence aptasensor for simultaneous determination of ochratoxin A and aflatoxin B1[J]. Analytica Chimica Acta, 2018, 1 019: 119-127.
[26] YAO L, CHEN Y, TENG J, et al. Integrated platform with magnetic purification and rolling circular amplification for sensitive fluorescent detection of ochratoxin A[J]. Biosensors and Bioelectronics, 2015, 74: 534-538.
[27] DAI S, WU S, DUAN N, et al. A near-infrared magnetic aptasensor for ochratoxin A based on near-infrared upconversion nanoparticles and magnetic nanoparticles[J]. Talanta, 2016, 158: 246-253.
[28] WU S, DUAN N, WANG Z, et al. Aptamer-functionalized magnetic nanoparticle-based bioassay for the detection of ochratoxin a using upconversion nanoparticles as labels[J]. Analyst, 2011, 136(11): 2 306-2 314.
[29] ZHANG J, ZHANG X, YANG G, et al. A signal-on fluorescent aptasensor based on Tb3+ and structure-switching aptamer for label-free detection of ochratoxin A in wheat[J]. Biosensors and Bioelectronics, 2013, 41: 704-709.
[30] CHEN Z, LIU C, CAO F, et al. DNA metallization: principles, methods, structures, and applications[J]. Chemical Society Reviews, 2018, 47(11): 4 017-4 072.
[31] CHEN J, ZHANG X, CAI S, et al. A fluorescent aptasensor based on DNA-scaffolded silver-nanocluster for ochratoxin A detection[J]. Biosensors and Bioelectronics, 2014, 57: 226-231.
[32] ZHANG J, XIA Y, CHEN M, et al. A fluorescent aptasensor based on DNA-scaffolded silver nanoclusters coupling with Zn (II)-ion signal-enhancement for simultaneous detection of OTA and AFB1[J]. Sensors and Actuators B: Chemical, 2016, 235: 79-85.
[33] HE Y, TIAN F, ZHOU J, et al. A fluorescent aptasensor for ochratoxin A detection based on enzymatically generated copper nanoparticles with a polythymine scaffold[J]. Microchimica Acta, 2019, 186(3): 199.[34] ZHAO Y, YANG Y, LUO Y, et al. Double detection of mycotoxins based on SERS labels embedded Ag@ Au core-shell nanoparticles[J]. ACS Applied Materials & Interfaces, 2015, 7(39): 21 780-21 786.
[35] SONG D, YANG R, FANG S, et al. SERS based aptasensor for ochratoxin A by combining Fe3O4@Au magnetic nanoparticles and Au-DTNB@Ag nanoprobes with multiple signal enhancement[J]. Microchimica Acta, 2018, 185(10): 491.
[36] SHAO B, MA X, ZHAO S, et al. Nanogapped Au (core)@ Au-Ag (shell) structures coupled with Fe3O4 magnetic nanoparticles for the detection of Ochratoxin A[J]. Analytica Chimica Acta, 2018, 1 033: 165-172.
[37] CHEN H, LIN M, WANG C, et al. Large-scale hot spot engineering for quantitative SERS at the single-molecule scale[J]. Journal of the American Chemical Society, 2015, 137(42): 13 698-13 705.
[38] BARTHELMEBS L, HAYAT A, LIMIADI A W, et al. Electrochemical DNA aptamer-based biosensor for OTA detection, using superparamagnetic nanoparticles[J]. Sensors and Actuators B: Chemical, 2011, 156(2): 932-937.
[39] RHOUATI A, HAYAT A, HERNANDEZ D B, et al. Development of an automated flow-based electrochemical aptasensor for on-line detection of ochratoxin A[J]. Sensors and Actuators B: Chemical, 2013, 176: 1 160-1 166.
[40] BONEL L, VIDAL J C, DUATO P, et al. An electrochemical competitive biosensor for ochratoxin A based on a DNA biotinylated aptamer[J]. Biosensors and Bioelectronics, 2011, 26(7): 3 254-3 259.
[41] WANG C, QIAN J, WANG K, et al. Nitrogen-doped graphene quantum dots@SiO2 nanoparticles as electrochemiluminescence and fluorescence signal indicators for magnetically controlled aptasensor with dual detection channels[J]. ACS Applied Materials & Interfaces, 2015, 7(48): 26 865-26 873.
[42] WANG C, QIAN J, AN K, et al. Magneto-controlled aptasensor for simultaneous electrochemical detection of dual mycotoxins in maize using metal sulfide quantum dots coated silica as labels[J]. Biosensors and Bioelectronics, 2017, 89: 802-809.
[43] HAO N, JIANG L, QIAN J, et al. Ultrasensitive electrochemical Ochratoxin A aptasensor based on CdTe quantum dots functionalized graphene/Au nanocomposites and magnetic separation[J]. Journal of Electroanalytical Chemistry, 2016, 781: 332-338.
[44] TONG P, ZHAO W, ZHANG L, et al. Double-probe signal enhancing strategy for toxin aptasensing based on rolling circle amplification[J]. Biosensors and Bioelectronics, 2012, 33(1): 146-151.
[45] SHI L, RONG X, WANG Y, et al. High-performance and versatile electrochemical aptasensor based on self-supported nanoporous gold microelectrode and enzyme-induced signal amplification[J]. Biosensors and Bioelectronics, 2018, 102: 41-48.
[46] MODH H, SCHEPER T, WALTER J. Detection of ochratoxin A by aptamer-assisted real-time PCR-based assay (Apta-qPCR)[J]. Engineering in Life Sciences, 2017, 17(8): 923-930.
[47] LISI F, PETERSON J R, GOODING J J. The application of personal glucose meters as universal point-of-care diagnostic tools[J]. Biosensors and Bioelectronics, 2019, 148: 111 835.
[48] GU C, LONG F, ZHOU X, et al. Portable detection of ochratoxin A in red wine based on a structure-switching aptamer using a personal glucometer[J]. RSC Advances, 2016, 6(35): 29 563-29 569.
[49] QIU S, YUAN L, WEI Y, et al. DNA template-mediated click chemistry-based portable signal-on sensor for ochratoxin A detection[J]. Food Chemistry, 2019, 297:124 929.
文章导航

/