食品与发酵工业 >

2020 , Vol. 46 >Issue 11: 307 - 314

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.023443

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基于磁性材料的适配体传感器在赭曲霉毒素超灵敏检测中的研究进展

  • 蔡小霞 ,
  • 苑静 ,
  • 林童 ,
  • 郭徐静 ,
  • 熊勇华 ,
  • 吴科盛 ,
  • 郭亮
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  • 1(南昌大学 食品科学与技术国家重点实验室,江西 南昌,330047)
    2(南昌大学 中德联合研究院,江西 南昌,330047)
    3(江西省兽药饲料监察所,江西 南昌,330029)
硕士研究生(郭亮助理研究员为通讯作者,E-mail:bioguo@163.com)

收稿日期: 2020-01-21

  网络出版日期: 2020-06-24

基金资助

“十三五”国家重点研发计划重点专项(2018YFC1602500);国家自然科学基金地区科学基金项目(31360385);江西省教育厅落地计划(KJLD13011)

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The progress of aptasensor based on magnetic material in ultratracedetection of ochratoxin

  • CAI Xiaoxia ,
  • YUAN Jing ,
  • LIN Tong ,
  • Guo Xujing ,
  • XIONG Yonghua ,
  • WU Kesheng ,
  • GUO Liang
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  • 1(State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)
    2(Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China)
    3(Jiangxi Province Institute of Veterinary Drug and Feed Control, Nanchang, 330029, China)

Received date: 2020-01-21

  Online published: 2020-06-24

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摘要

赭曲霉毒素A(ochratoxin A,OTA)毒性大、分布广,因此建立简便且适于复杂基质的超灵敏OTA检测方法十分必要。基于磁性材料的适配体传感器兼具适配体稳定性高和磁性材料磁操作简便的特点,可实现待测物或检测探针的快速分离富集,有效提高检测灵敏度并缩短检测时间,适于食品中痕量OTA的检测。该文按输出信号类型的不同综述了近年来基于磁性材料的OTA适配体传感器的研究进展。着重阐述了磁性材料、新型纳米材料和生物技术在放大检测信号、提高磁性适配体传感器检测灵敏度中的应用及其机制,旨在为适配体传感器的设计和真菌毒素的痕量检测提供参考依据。

关键词： 适配体; 传感器; 磁性材料; 赭曲霉毒素; 超灵敏检测

本文引用格式

蔡小霞 , 苑静 , 林童 , 郭徐静 , 熊勇华 , 吴科盛 , 郭亮 . 基于磁性材料的适配体传感器在赭曲霉毒素超灵敏检测中的研究进展[J]. 食品与发酵工业, 2020 , 46(11) : 307 -314 . DOI: 10.13995/j.cnki.11-1802/ts.023443

Abstract

Due to the high toxicity and wide distribution of (ochratoxin A,OTA), it's essential to establish facile and ultrasensitive methods for the detection of OTA in complex matrices. Benefiting from the high stability of aptamer and easy magnetic handling of magnetic material, the magnetic material-based aptasensor could achieve a rapid magnetic separation and enrichment of analytes or detection probes to improve the detection sensitivity and shorten the detection duration effectively which made it extremely suitable for the detection of trace OTA in a food sample. Herein, the recent progress in magnetic material-based OTA aptasensor using different output signals was summarized, with special emphasis on the applications of magnetic materials, novel nanomaterials and biotechnology, as well as their mechanisms in amplifying detection signals and improving the detection sensitivities, aiming at providing a reference for the design of aptasensor and ultratrace detection of mycotoxin.

Key words： aptamer; sensor; magnetic meterial; ochratoxin; ultrasensitive detection

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