研究报告

缫丝蚕蛹与黄豆的纳豆菌发酵上清液功能活性的比较

  • 卢美杉 ,
  • 朱蓓薇 ,
  • 付云 ,
  • 刘小玲
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  • 1(广西大学 轻工与食品工程学院,广西 南宁,530004)
    2(国家海洋食品工程技术研究中心,辽宁 大连,116034)
硕士研究生(刘小玲教授为通讯作者,E-mail:1106133912@qq.com)

收稿日期: 2020-02-24

  网络出版日期: 2020-07-15

基金资助

广西重大基金项目(2016GXNSFEA380003);广西创新驱动发展重大专项(桂科AA17204075)

Functional activity comparison of silkworm chrysalis and soybean fermentation broths produced by Bacillus natto

  • LU Meishan ,
  • ZHU Beiwei ,
  • FU Yun ,
  • LIU Xiaoling
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  • 1(College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China)
    2(National Engineering Research Center of Seafood, Dalian 116034, China)

Received date: 2020-02-24

  Online published: 2020-07-15

摘要

蚕蛹是我国“食品新资源”的一种,也是仅有的昆虫类原料。该文以缫丝副产物—新鲜蚕蛹为研究对象,接种纳豆菌(Bacillus natto)发酵并探讨其发酵产物的生物活性,期望发掘蚕蛹的新功能和新价值。研究表明,蚕蛹蛋白质、脂肪含量极高,氨基酸组成与黄豆相似,纳豆菌在蚕蛹培养基和黄豆培养基中的生长曲线相似。煮熟蚕蛹发酵液的抗氧化性高于新鲜蚕蛹,在发酵32 h时ABTS自由基清除率达到96.8%,降血脂和抗凝血活性及纳豆激酶活力比鲜蛹发酵液低。2种蚕蛹发酵液在抗氧化活性、降血脂活性和抗凝血活性上的表现均优于黄豆发酵液,其中,蚕蛹发酵液在32 h时3种胆酸盐结合率都在75%以上。综上,蚕蛹纳豆菌发酵产物具有显著的优势,该研究是实现缫丝蚕蛹高价值利用的重要途径。

本文引用格式

卢美杉 , 朱蓓薇 , 付云 , 刘小玲 . 缫丝蚕蛹与黄豆的纳豆菌发酵上清液功能活性的比较[J]. 食品与发酵工业, 2020 , 46(12) : 109 -115 . DOI: 10.13995/j.cnki.11-1802/ts.023739

Abstract

Silkworm chrysalis is a “new food source” in China, and also the only insect-derived food material. In this study, we fermented silkworm chrysalis, a silk reeling byproduct, using Bacillus natto, and then examined the biological activity of the fermentation product, aiming to explore new functions and values of silkworm chrysalis. The results showed that silkworm chrysalis was rich in protein and fat, and its amino acid composition was similar to that of soybean. Moreover, the growth curve of Bacillus natto was highly similar in both media (soybean and silkworm chrysalis). The fermentation broth of the boiled silkworm chrysalis had higher antioxidant activity than that of fresh silkworm chrysalis, with an ABTS clearance rate reaching 96.8% after 32 h of fermentation; however, its antilipemic and anticoagulant activities were lower. Both types of silkworm chrysalis fermentation broths had superior anti-oxidant, antilipemic, and anticoagulant activities compared to the soybean fermentation broth. Among these properties, silkworm chrysalis fermentation broths exhibited binding rates above 75% to three types of cholic acid after 32 h of fermentation. In conclusion, the Bacillus natto fermentation product of silkworm chrysalis showed significant advantages. Our study highlights an important method for the high value utilization of silkworm chrysalis.

参考文献

[1] 周志峰.物理改性对蚕蛹蛋白功能特性及酶解特性的影响[D]. 镇江:江苏科技大学, 2017.
[2] 李勇, 王文昕.蚕蛹蛋白提取及其多肽生理功能研究进展[J].粮食与油脂, 2010(10):44-46.
[3] 陈义安, 刘俊凤, 张友洪, 等.不同品种桑蚕蛹氨基酸含量差异及其评价[J].贵州农业科学, 2018,46(2):89-92.
[4] 季晓娇, 闫文杰, 张婧婕, 等.蚕蛹蛋白制备应用与功能特性的研究进展[J].食品与发酵工业,2019,45(18):277-283.
[5] 刘静, 徐立, 黄先智.蚕蛹的生物活性成分及药理作用研究进展[J].食品科学, 2012,33(17):303-307.
[6] 孔繁东, 鲁玉.蚕蛹纳豆菌发酵物抗菌性研究[J].食品工业科技, 2010,31(11):213-215.
[7] 龙厚宁, 张硕, 姚磊, 等.纳豆菌液体发酵转化黄芩苷和汉黄芩苷的工艺研究[J].中国中药杂志, 2015, 40(23):4 623-4 628.
[8] 李健, 蒋艳忠.碱法提取蚕蛹蛋白的工艺研究[J].食品研究与开发, 2016,37(9):52-54.
[9] 穆利霞, 廖森泰, 詹宝瑟, 等.超声处理对碱法制备蚕蛹蛋白条件的优化[J].热带作物学报, 2014,35(5):1 005-1 011.
[10] 王伟, 何国庆, 金英哲, 等.蚕蛹蛋白的综合利用现状分析和开发前景展望[J].食品与发酵工业, 2006,32(9):112-115.
[11] 练钊.繅丝蚕蛹蛋白功能性质及谷蛋白糖基化修饰的研究[D].南宁:广西大学, 2018.
[12] WEI X, LUO M, XU L, et al.Production of fibrinolytic enzyme from Bacillus amyloliquefaciens by fermentation of chickpeas, with the evaluation of the anticoagulant and antioxidant properties of chickpeas[J].Journal of Agricultural and Food Chemistry, 2011,59(8):3 957-3 963.
[13] CAI D, WEI X, QIU Y, et al.High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase[J].Journal of Applied Microbiology, 2016,121(3):704-712.
[14] 法芸, 张金玲, 赵海杰, 等.纳豆激酶分离纯化和酶活性测定的研究进展[J].色谱, 2019,37(3):274-278.
[15] DAI C, MA H, HE R, et al.Improvement of nutritional value and bioactivity of soybean meal by solid-state fermentation with Bacillus subtilis[J].LWT-Food Science and Technology, 2017,86:1-7.
[16] QUEIROZ SANTOS V A, NASCIMENTO C G, SCHMIDT C A P, et al.Solid-state fermentation of soybean okara: Isoflavones biotransformation, antioxidant activity and enhancement of nutritional quality[J].LWT-Food Science and Techology, 2018,92:509-515.
[17] 王立博, 陈复生.黄豆活性肽生理保健功能研究进展[J].食品与机械, 2016,32(2):198-201.
[18] 刘山, 李冬梅, 许喆, 等.裙带菜孢子叶多糖体外降血脂活性的研究[J].食品工业, 2016,37(11):177-179.
[19] 王静辉, 王倩, 高林林, 等.山稔子提取物降血脂作用的研究[J].食品科技, 2018,43(2):235-240.
[20] ZHOU K, XIA W, ZHANG C, et al.In vitro binding of bile acids and triglycerides by selected chitosan preparations and their physico-chemical properties[J].LWT - Food Science and Technology, 2006,39(10):1 087-1 092.
[21] 于美汇, 赵鑫, 尹红力, 等.碱提醇沉黑木耳多糖体外和体内降血脂功能[J].食品科学, 2017,38(1):232-237.
[22] 李岩, 张翠香, 罗永会, 等.云南产地参多糖脱色、脱蛋白及其抗凝血活性研究[J].安徽农学通报, 2019,25(7):13-16.
[23] 王程.蜈蚣提取物抗肝损伤、抗肿瘤、抗凝血作用及其急性毒性研究[D].武汉:湖北中医药大学, 2018.
[24] 李为喜, 朱志华, 刘三才, 等.中国黄豆(Glycine max)品种及种质资源主要品质状况分析[J].植物遗传资源学报, 2004,5(2):185-192.
[25] 蒋涛, 杨文钰, 刘卫国, 等.套作大豆贮藏蛋白、氨基酸组成分析及营养评价[J].食品科学, 2012,33(21):275-279.
[26] 黄惠华, 高孔荣, 郭乾初, 等.大豆原料及其分离蛋白的SDS-PAGE图谱研究[J].食品科学, 2000,21(6):15-19.
[27] 吕永宁, 陈东生, 徐楚鸿.菝葜活血化瘀药理作用[J].中国医院药学杂志, 2002, 22(9):538-540.
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