研究报告

西北酸菜中吸附黄曲霉毒素B1乳酸菌株的筛选鉴定及稳定性研究

  • 唐璎 ,
  • 孟宪刚 ,
  • 邓展瑞 ,
  • 马芮萍 ,
  • 黄佳
展开
  • 1(甘肃省产品质量监督检验研究院,甘肃 兰州,730050)
    2(兰州交通大学 化学与生物工程学院,甘肃 兰州,730070
硕士,工程师(孟宪刚教授为通讯作者,E-mail:mengxg@mail.lzjtu.cn)

收稿日期: 2020-04-13

  修回日期: 2020-05-11

  网络出版日期: 2020-08-15

Screening and stability of an aflatoxin B1-adsorbing lactic acid bacterium isolated from pickled vegetable in northwest China

  • TANG Ying ,
  • MENG Xiangang ,
  • DENG Zhanrui ,
  • MA Ruiping ,
  • HUANG Jia
Expand
  • 1(Gansu Province Product Quality Supervision and Inspection Research Institute,Lanzhou 730050,China)
    2(School of Chemical and Biological Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China)

Received date: 2020-04-13

  Revised date: 2020-05-11

  Online published: 2020-08-15

摘要

为工业化控制黄曲霉毒素B1(aflatoxin B1,AFB1)污染,筛选吸附率高、稳定性好、脱毒后具备食品安全的优势菌株, 应用超高效液相色谱-三重四级杆串联质谱(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)技术检测菌株发酵液AFB1残留量的方法,从35份采集自甘肃不同地域的西北酸菜样品中筛选到1株活菌脱毒率为66.41%、解吸后仍保留脱毒率为53.7%的菌株L34-2。菌株L34-2经形态学、生理生化及16S rRNA基因序列扩增测序,鉴定为布氏乳杆菌(Lactobacillus buchneri)。通过磷酸缓冲盐溶液(phosphate buffer saline,PBS)洗脱,体外模拟胃液消化,研究菌株-AFB1复合体稳定性,结果表明,菌株L34-2吸附AFB1具有较好的稳定性。

本文引用格式

唐璎 , 孟宪刚 , 邓展瑞 , 马芮萍 , 黄佳 . 西北酸菜中吸附黄曲霉毒素B1乳酸菌株的筛选鉴定及稳定性研究[J]. 食品与发酵工业, 2020 , 46(15) : 60 -65 . DOI: 10.13995/j.cnki.11-1802/ts.024201

Abstract

In order to solve the problem of industrial large-scale application of biological method to remove aflatoxin B1(AFB1),an AFB1-adsorptive strain L34-2 was isolated from pickled vegetable in northwest China and its AFB1 detoxification rate was 66.41%. Based on the morphological,physiological,biochemical analysis and sequence alignment of 16S rRNA,it was identified as Lactobacillus buchneri. The stability of the bacteria-AFB1complex was studied by phosphate buffer saline(PBS) elution and in vitro gastric juice digestion simulation. The results showed that it had good stability in adsorption of AFB1.

参考文献

[1] 罗自生,秦雨,徐艳群,等.黄曲霉毒素的生物合成、代谢和毒性研究进展[J].食品科学,2015,36(3):250-257.
[2] 赵萌,高婧,褚华硕,等.黄曲霉毒素B1的分子致毒机理及其微生物脱毒研究进展[J].食品科学,2019,40(11):235-245.
[3] 乔宏兴,姜亚乐,王永芬,等.黄曲霉毒素的危害及其脱毒方法研究进展[J].动物医学进展,2017,38(1):89-93.
[4] LIU Ruijie,WANG Ruiqi,LU Jian,et al.Degradation of AFB1 in aqueous medium by electron beam irradiation:Kinetics,pathway and toxicology[J].Food Control,2016,66:151-157.
[5] MOON Y S,KIM H M,CHUN H S,et al.Organic acids suppress aflatoxin production via lowering expression of aflatoxin biosynthesis-related genes in Aspergillus flavus[J].Food Control,2018,88:207-216.
[6] RASTEGAR H,SHOEIBI S,YAZDANPANAH H,et al.Removal of aflatoxin B1 by roasting with lemon juice and/or citric acid in contaminated pistachio nuts[J].Food Control,2017,71:279-284.
[7] 蔡俊,田尔诺,邵帅,等.黄曲霉毒素B1生物脱毒的研究进展[J].微生物学通报,2017,44(3):726-731.
[8] VERHEECKE C,LIBOZ T,MATHIEU F.Microbial degradation of aflatoxin B1: Current status and future advances[J].International Journal of Food Microbiology,2016,237:1-9.
[9] ADEBO O A,NJOBEH P B,GBASHI S,et al.Review on microbial degradation of aflatoxins[J].Critical Reviews in Food Science and Nutrition,2017,57(15):3 208-3 217.
[10] XU L,AHMED M F E,SANGARE L,et al. Novel aflatoxin-degrading enzyme from Bacillus shackletonii L7[J].Toxins,2017,9(1):36.
[11] SHUKLA S,PARK J H,CHUNG S H,et al.Ochratoxin a reduction ability of biocontrol agent Bacillus subtilis isolated from korean traditional fermented food kimchi.[J].Scientific reports,2018,8(1):8 039.
[12] TAHEUR F B,FEDHILA K,CHAIEB K,et al.Adsorption of aflatoxin B1,zearalenone and ochratoxin A by microorganisms isolated from Kefir grains[J]. International Journal of Food Microbiology,2017,251:1-7.
[13] 黄丽. 植物乳杆菌C88干预黄曲霉毒素B1毒性的分子机制[D].长春:东北师范大学,2019.
[14] CARLOS A F O,FERNANDA B,CARLOS H C,et al.Recent trends in microbiological decontamination of aflatoxins in foodstuffs[J].Recent Advances and Future Prospects,2013,2013:59-92.
[15] 刘畅,刘阳,邢福国,等.黄曲霉毒素B1吸附菌株的筛选及吸附机理研究[J].核农学报,2010,24(4):766-771.
[16] XIA Xiaoshuang,ZHANG Ye,LI Mingyan,et al.Isolation and characterization of a Bacillus subtilis strain with aflatoxin B1 biodegradation capability[J].Food Control,2017,75:92-98.
[17] 李超波,李文明,杨文华,等.黄曲霉毒素B1降解菌的分离鉴定及其降解特性[J].微生物学报,2012,52(9):1 129-1 136.
[18] 孟繁磊,宋志峰,牛红红,等.基于UPLC-MS-MS技术快速测定花生中4种黄曲霉毒素[J].中国油脂,2018,43(1):136-139.
[19] 邵帅,戴军,杜馨,等.降解黄曲霉毒素B1菌株的发酵条件优化及降解机制[J].食品科学,2016,37(5):138-143.
[20] GARRITY G M,BELL J A,LILBURN T G.Bergey’s Manual of Systematic Bacteriology[M]. Ninth Edition. New York: Springer New York Berlin Herdelberg,2004.
[21] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:318-419.
[22] HERNANDEZ-MENDOZA A,GUZMAN-DE-PENA D,GARCIAH S.Key role of teichoic acids on aflatoxin B1 binding by probiotic bacteria[J].Journal of Applied Microbiology,2009,107(2):395-403.
[23] Kim S,Lee H,Lee S,et al.Invited review:Microbe-mediated aflatoxin decontamination of dairy products and feeds[J]. Journal of Dairy Science,2017,100(2):871-880.
[24] 田申,李贻杰,曹文红,等.利用虾头内源酶模拟胃肠道消化制备短肽的研究[J].食品工业科技,2016,37(9):92-96.
[25] 王宁. 基于菌藻联合策略防控刺参圈水绵的研究[D].大连:大连理工大学,2019.
[26] 凌代文. 乳酸细菌分类鉴定及实验方法[J].微生物学通报,1999(1):23.
文章导航

/