食品与发酵工业 >

2020 , Vol. 46 >Issue 15: 60 - 65

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024201

研究报告

西北酸菜中吸附黄曲霉毒素B1乳酸菌株的筛选鉴定及稳定性研究

  • 唐璎 ,
  • 孟宪刚 ,
  • 邓展瑞 ,
  • 马芮萍 ,
  • 黄佳
展开
  • 1(甘肃省产品质量监督检验研究院,甘肃 兰州,730050)
    2(兰州交通大学 化学与生物工程学院,甘肃 兰州,730070
硕士,工程师(孟宪刚教授为通讯作者,E-mail：mengxg@mail.lzjtu.cn)

收稿日期: 2020-04-13

  修回日期: 2020-05-11

  网络出版日期: 2020-08-15

收起

Screening and stability of an aflatoxin B1-adsorbing lactic acid bacterium isolated from pickled vegetable in northwest China

  • TANG Ying ,
  • MENG Xiangang ,
  • DENG Zhanrui ,
  • MA Ruiping ,
  • HUANG Jia
Expand
  • 1(Gansu Province Product Quality Supervision and Inspection Research Institute,Lanzhou 730050,China)
    2(School of Chemical and Biological Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China)

Received date: 2020-04-13

  Revised date: 2020-05-11

  Online published: 2020-08-15

Fold

摘要

为工业化控制黄曲霉毒素B1(aflatoxin B1,AFB1)污染,筛选吸附率高、稳定性好、脱毒后具备食品安全的优势菌株, 应用超高效液相色谱-三重四级杆串联质谱(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)技术检测菌株发酵液AFB1残留量的方法,从35份采集自甘肃不同地域的西北酸菜样品中筛选到1株活菌脱毒率为66.41%、解吸后仍保留脱毒率为53.7%的菌株L34-2。菌株L34-2经形态学、生理生化及16S rRNA基因序列扩增测序,鉴定为布氏乳杆菌(Lactobacillus buchneri)。通过磷酸缓冲盐溶液(phosphate buffer saline,PBS)洗脱,体外模拟胃液消化,研究菌株-AFB1复合体稳定性,结果表明,菌株L34-2吸附AFB1具有较好的稳定性。

关键词： 黄曲霉毒素B1; 乳酸菌; 超高效液相色谱-三重四级杆串联质谱; 西北酸菜; 模拟体外胃液消化

本文引用格式

唐璎 , 孟宪刚 , 邓展瑞 , 马芮萍 , 黄佳 . 西北酸菜中吸附黄曲霉毒素B1乳酸菌株的筛选鉴定及稳定性研究[J]. 食品与发酵工业, 2020 , 46(15) : 60 -65 . DOI: 10.13995/j.cnki.11-1802/ts.024201

Abstract

In order to solve the problem of industrial large-scale application of biological method to remove aflatoxin B1(AFB1),an AFB1-adsorptive strain L34-2 was isolated from pickled vegetable in northwest China and its AFB1 detoxification rate was 66.41%. Based on the morphological,physiological,biochemical analysis and sequence alignment of 16S rRNA,it was identified as Lactobacillus buchneri. The stability of the bacteria-AFB1complex was studied by phosphate buffer saline(PBS) elution and in vitro gastric juice digestion simulation. The results showed that it had good stability in adsorption of AFB1.

Key words： aflatoxin B1; lactic acid bacteria; UPLC-MS/MS; pickled vegetable in northwest China; simulates in vitro gastric juice digestion

参考文献

[1] 罗自生,秦雨,徐艳群,等.黄曲霉毒素的生物合成、代谢和毒性研究进展[J].食品科学,2015,36(3):250-257.
[2] 赵萌,高婧,褚华硕,等.黄曲霉毒素B1的分子致毒机理及其微生物脱毒研究进展[J].食品科学,2019,40(11):235-245.
[3] 乔宏兴,姜亚乐,王永芬,等.黄曲霉毒素的危害及其脱毒方法研究进展[J].动物医学进展,2017,38(1):89-93.
[4] LIU Ruijie,WANG Ruiqi,LU Jian,et al.Degradation of AFB1 in aqueous medium by electron beam irradiation:Kinetics,pathway and toxicology[J].Food Control,2016,66:151-157.
[5] MOON Y S,KIM H M,CHUN H S,et al.Organic acids suppress aflatoxin production via lowering expression of aflatoxin biosynthesis-related genes in Aspergillus flavus[J].Food Control,2018,88:207-216.
[6] RASTEGAR H,SHOEIBI S,YAZDANPANAH H,et al.Removal of aflatoxin B1 by roasting with lemon juice and/or citric acid in contaminated pistachio nuts[J].Food Control,2017,71:279-284.
[7] 蔡俊,田尔诺,邵帅,等.黄曲霉毒素B1生物脱毒的研究进展[J].微生物学通报,2017,44(3):726-731.
[8] VERHEECKE C,LIBOZ T,MATHIEU F.Microbial degradation of aflatoxin B1: Current status and future advances[J].International Journal of Food Microbiology,2016,237:1-9.
[9] ADEBO O A,NJOBEH P B,GBASHI S,et al.Review on microbial degradation of aflatoxins[J].Critical Reviews in Food Science and Nutrition,2017,57(15):3 208-3 217.
[10] XU L,AHMED M F E,SANGARE L,et al. Novel aflatoxin-degrading enzyme from Bacillus shackletonii L7[J].Toxins,2017,9(1):36.
[11] SHUKLA S,PARK J H,CHUNG S H,et al.Ochratoxin a reduction ability of biocontrol agent Bacillus subtilis isolated from korean traditional fermented food kimchi.[J].Scientific reports,2018,8(1):8 039.
[12] TAHEUR F B,FEDHILA K,CHAIEB K,et al.Adsorption of aflatoxin B1,zearalenone and ochratoxin A by microorganisms isolated from Kefir grains[J]. International Journal of Food Microbiology,2017,251:1-7.
[13] 黄丽. 植物乳杆菌C88干预黄曲霉毒素B1毒性的分子机制[D].长春:东北师范大学,2019.
[14] CARLOS A F O,FERNANDA B,CARLOS H C,et al.Recent trends in microbiological decontamination of aflatoxins in foodstuffs[J].Recent Advances and Future Prospects,2013,2013:59-92.
[15] 刘畅,刘阳,邢福国,等.黄曲霉毒素B1吸附菌株的筛选及吸附机理研究[J].核农学报,2010,24(4):766-771.
[16] XIA Xiaoshuang,ZHANG Ye,LI Mingyan,et al.Isolation and characterization of a Bacillus subtilis strain with aflatoxin B1 biodegradation capability[J].Food Control,2017,75:92-98.
[17] 李超波,李文明,杨文华,等.黄曲霉毒素B1降解菌的分离鉴定及其降解特性[J].微生物学报,2012,52(9):1 129-1 136.
[18] 孟繁磊,宋志峰,牛红红,等.基于UPLC-MS-MS技术快速测定花生中4种黄曲霉毒素[J].中国油脂,2018,43(1):136-139.
[19] 邵帅,戴军,杜馨,等.降解黄曲霉毒素B1菌株的发酵条件优化及降解机制[J].食品科学,2016,37(5):138-143.
[20] GARRITY G M,BELL J A,LILBURN T G.Bergey’s Manual of Systematic Bacteriology[M]. Ninth Edition. New York: Springer New York Berlin Herdelberg,2004.
[21] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:318-419.
[22] HERNANDEZ-MENDOZA A,GUZMAN-DE-PENA D,GARCIAH S.Key role of teichoic acids on aflatoxin B1 binding by probiotic bacteria[J].Journal of Applied Microbiology,2009,107(2):395-403.
[23] Kim S,Lee H,Lee S,et al.Invited review:Microbe-mediated aflatoxin decontamination of dairy products and feeds[J]. Journal of Dairy Science,2017,100(2):871-880.
[24] 田申,李贻杰,曹文红,等.利用虾头内源酶模拟胃肠道消化制备短肽的研究[J].食品工业科技,2016,37(9):92-96.
[25] 王宁. 基于菌藻联合策略防控刺参圈水绵的研究[D].大连:大连理工大学,2019.
[26] 凌代文. 乳酸细菌分类鉴定及实验方法[J].微生物学通报,1999(1):23.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司