综合主成分分析、偏最小二乘回归分析、通径分析以及相关分析探讨1-甲基环丙烯(1-methylcyclopropene,1-MCP)处理对牛心甘蓝贮藏特性的影响。结果表明,1-MCP处理可推迟组织中叶绿素、总硫代葡萄糖苷、萝卜硫素、叶酸含量的下降,抑制丙二醛和亚硝酸盐的积累; 在1-MCP处理甘蓝中,乙烯释放和呼吸强度也得以降低;此外,1-MCP处理维持了组织中较高的抗坏血酸、总酚及抗氧化能力。主成分分析表明,1-MCP对叶绿素、总硫代葡萄糖苷、叶酸、抗坏血酸、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率及羟自由基(·OH)清除率、丙二醛和亚硝酸盐均有影响;由偏最小二乘分析可知,甘蓝叶绿素与叶酸、抗坏血酸、DPPH·清除率、总硫代葡萄糖苷、·OH清除率正相关,与丙二醛呈负相关;由通径分析可知,DPPH·清除率主要通过自身的直接作用和叶酸、抗坏血酸及·OH清除率的间接作用来影响甘蓝的叶绿素水平。综上所述,1-MCP对牛心甘蓝采后贮藏特性的影响主要与其维持组织的抗氧化能力有关。
The effects of 1-methylcyclopropene (1-MCP) treatment on storage characteristics of heart-shaped cabbage were investigated by principal component analysis, partial least square regression analysis, path analysis and correlation analysis. The results showed that 1-MCP could delay the decrease of chlorophyll, glucosinolates, sulforaphane and folate, and inhibit the accumulation of malondialdehyde (MDA) and nitrite. Ethylene production and respiratory intensity were also reduced in 1-MCP-treated cabbage. Additionally, 1-MCP treatment maintained higher ascorbic acid, total phenol and antioxidant capacity in tissues. Moreover, principal component analysis indicated that 1-MCP had effects on chlorophyll, total glucosinolates, folic acid, ascorbic acid, DPPH· scavenger activity, ·OH scavenger activity, MDA, and nitrite. The partial least square analysis displayed that the chlorophyll of cabbage was positively correlated with folic acid, ascorbic acid, DPPH· scavenger activity, total glucosinolates, ·OH scavenger activity, and negatively correlated with MDA. Furthermore, the results of path analysis manifested that DPPH scavenger activity affected the chlorophyll level of cabbage mainly through its direct action and indirect action of folic acid, ascorbic acid and ·OH scavenger activity. In conclusion, the effect of 1-MCP on postharvest storage characteristics of heart-shaped cabbage was mainly related to its ability to maintain tissue antioxidant capacity.
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