综合主成分分析、偏最小二乘回归分析、通径分析以及相关分析探讨1-甲基环丙烯(1-methylcyclopropene,1-MCP)处理对牛心甘蓝贮藏特性的影响。结果表明,1-MCP处理可推迟组织中叶绿素、总硫代葡萄糖苷、萝卜硫素、叶酸含量的下降,抑制丙二醛和亚硝酸盐的积累; 在1-MCP处理甘蓝中,乙烯释放和呼吸强度也得以降低;此外,1-MCP处理维持了组织中较高的抗坏血酸、总酚及抗氧化能力。主成分分析表明,1-MCP对叶绿素、总硫代葡萄糖苷、叶酸、抗坏血酸、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率及羟自由基(·OH)清除率、丙二醛和亚硝酸盐均有影响;由偏最小二乘分析可知,甘蓝叶绿素与叶酸、抗坏血酸、DPPH·清除率、总硫代葡萄糖苷、·OH清除率正相关,与丙二醛呈负相关;由通径分析可知,DPPH·清除率主要通过自身的直接作用和叶酸、抗坏血酸及·OH清除率的间接作用来影响甘蓝的叶绿素水平。综上所述,1-MCP对牛心甘蓝采后贮藏特性的影响主要与其维持组织的抗氧化能力有关。
The effects of 1-methylcyclopropene (1-MCP) treatment on storage characteristics of heart-shaped cabbage were investigated by principal component analysis, partial least square regression analysis, path analysis and correlation analysis. The results showed that 1-MCP could delay the decrease of chlorophyll, glucosinolates, sulforaphane and folate, and inhibit the accumulation of malondialdehyde (MDA) and nitrite. Ethylene production and respiratory intensity were also reduced in 1-MCP-treated cabbage. Additionally, 1-MCP treatment maintained higher ascorbic acid, total phenol and antioxidant capacity in tissues. Moreover, principal component analysis indicated that 1-MCP had effects on chlorophyll, total glucosinolates, folic acid, ascorbic acid, DPPH· scavenger activity, ·OH scavenger activity, MDA, and nitrite. The partial least square analysis displayed that the chlorophyll of cabbage was positively correlated with folic acid, ascorbic acid, DPPH· scavenger activity, total glucosinolates, ·OH scavenger activity, and negatively correlated with MDA. Furthermore, the results of path analysis manifested that DPPH scavenger activity affected the chlorophyll level of cabbage mainly through its direct action and indirect action of folic acid, ascorbic acid and ·OH scavenger activity. In conclusion, the effect of 1-MCP on postharvest storage characteristics of heart-shaped cabbage was mainly related to its ability to maintain tissue antioxidant capacity.
[1] HAN N, KU K M, KIM J. Postharvest variation of major glucosinolate and their hydrolytic products in Brassicoraphanus ‘BB1’[J]. Postharvest Biology and Technology, 2019, 154: 70-78.
[2] 曹帅颖,李洋,何晨阳, 等. 热处理及贮藏温度对紫甘蓝花色苷稳定性和抗氧化能力的影响[J].食品研究与开发, 2018, 39(2): 21-27.
[3] BERARD L S. Effects of harvest dates, field frost, and preservative coating on storage disorders of cabbage[J]. Hortscience, 1987, 22: 1 140-1 140.
[4] LEE Y J, HA J Y, OH J E, et al. The effect of LED irradiation on the quality of cabbage stored at a low temperature[J]. Food Science and Biotechnology, 2014, 23: 1 087-1 093.
[5] GOMEZ-LOBATO M E, HASPERUE J H, CIVELLO P M, et al. Effect of 1-MCP on the expression of chlorophyll degrading genes during senescence of broccoli (Brassica oleracea L.)[J]. Scientia Horticulturae, 2012, 144: 208-211.
[6] AL-UBEED H M S, WILLS R B H, BOWYER M C, et al. Comparison of hydrogen sulphide with 1-methylcyclopropene (1-MCP) to inhibit senescence of the leafy vegetable, pak choy[J]. Postharvest Biology and Technology, 2018, 137: 129-133.
[7] HASSAN F A S, MAHFOUZ S A. Effect of 1-methylcyclopropene (1-MCP) on the postharvest senescence of coriander leaves during storage and its relation to antioxidant enzyme activity[J]. Scientia Horticulturae, 2012, 141: 69-75.
[8] MASSOLO J F, FORTE L G, CONCELLON A, et al. Effects of ethylene and 1-MCP on quality maintenance of fresh cut celery[J]. Postharvest Biology and Technology, 2019, 148: 176-183.
[9] MENG J, ZHOU Q, ZHOU X, et al. Ethylene and 1-MCP treatments affect leaf abscission and associated metabolism of Chinese cabbage[J]. Postharvest Biology and Technology, 2019, 157: 110963.
[10] PORTER K L, COLLINS G, KLIEBER A. 1-MCP does not improve the shelf-life of Chinese cabbage[J]. Journal of The Science of Food and Agriculture, 2005, 85: 293-296.
[11] 刘红艳, 胡花丽, 罗淑芬, 等. 6-苄氨基嘌呤处理对鲜切西兰花品质的影响[J]. 江苏农业学报, 2018, 34(1): 186-193.
[12] 王瑶, 罗淑芬, 胡花丽, 等. 外源NO处理对采后鲜莲子品质及乙烯代谢的影响[J]. 现代食品科技, 2019, 35(11):100-108.
[13] 高建晓, 刘丹, 古荣鑫, 等. 6-苄氨基嘌呤处理对上海青贮藏品质的影响[J].食品科学, 2015, 36(4): 247-253.
[14] WEI J, MIAO H, WANG Q. Effect of glucose on glucosinolates, antioxidants and metabolic enzymes in brassica sprouts[J]. Scientia Horticulturae, 2011, 129(4): 535-540.
[15] GUO R, YUAN G, WANG Q. Sucrose enhances the accumulation of anthocyanins and glucosinolates in broccoli sprouts[J]. Food Chemistry, 2011, 129(3): 1 080-1 087.
[16] 刘红艳,胡花丽,罗淑芬,等. 6-苄氨基嘌呤减缓鲜切西兰花的衰老机理分析[J].食品科学, 2016, 37(24): 313-321.
[17] 张毅. 叶菜中叶酸含量的HPLC测定条件优化及不同处理方式对叶酸保留效果研究[D]. 杨凌:西北农林科技大学, 2016.
[18] 史君彦, 高丽朴, 左进华, 等. 纳米膜和PVC膜包装对西兰花贮藏保鲜的影响[J]. 食品工业科技, 2016, 37(19): 255-258.
[19] 王亚楠, 胡花丽, 古荣鑫, 等. 不同薄膜包装对桑葚采后品质的影响[J]. 食品科学, 2014, 35(18): 224-229.
[20] JING G X, HUANG H, YANG B, et al. Effect of pyrogallol on the physiology and biochemistry of litchi fruit during storage[J]. Chemistry Central Journal, 2013, 7: 1-11.
[21] SUN B, YAN H Z, LIU N, et al. Effect of 1-MCP treatment on postharvest quality characters, antioxidants and glucosinolates of Chinese kale[J]. Food Chemistry, 2012, 131: 519-526.
[22] FARCUH M, TOUBIANA D, SADE N, et al. Hormone balance in a climacteric plum fruit and its non-climacteric bud mutant during ripening[J]. Plant Science, 2019, 280: 51-65.
[23] CIESLIK E, LESZCZYNSKA T, FILIPIAK-FLORKIEWICZ A, et al. Effects of some technological processes on glucosinolate contents in cruciferous vegetables[J]. Food Chemistry, 2007, 105: 976-981.
[24] YUAN G F, SUN B, YUAN J, et al. Effect of 1-methylcyclopropene on shelf life, visual quality, antioxidant enzymes and health-promoting compounds in broccoli florets[J]. Food Chemistry, 2010, 118: 774-781.
[25] 王友升, 张萌, 王丹, 等. 减压处理对草莓贮藏效果及活性氧代谢影响的多变量解析[J]. 中国食品学报, 2015, 15(6): 231-238.
