评价共轭亚油酸(conjugated linoleic acid, CLA)作为功能组分在肠炎配方食品中的应用效果,同时研究基于益生菌在结肠原位产生CLA对慢性肠炎的缓解作用。选取雄性SPF级C57BL / 6J(雄性,8 周龄,18~20 g)小鼠50 只,随机分为对照组、模型组、实验组〔包括营养配方组B、营养配方组C(含体积分数1%CLA)、营养配方组P(含1×109 CFU/mL的益生菌)〕,每组10 只。除对照组外,在小鼠饮用水中添加质量浓度20~30 g/L葡聚糖硫酸钠(dextran sulfate sodium, DSS)进行3个周期诱导慢性结肠炎;分别喂养营养配方2 周,以体质量、结肠长度、髓过氧化物酶(myeloperoxidase, MPO)、病理切片、炎症因子、肠道菌群等作为评价指标,评估不同营养配方对慢性肠炎小鼠恢复的影响。与模型组小鼠相比,经各配方饲养后均能显著地改善小鼠的慢性肠炎病症。其中,添加CLA或益生菌的营养配方组小鼠分别在结肠长度、促炎因子的表达和体质量MPO、菌群调节等指标中效果更为显著。营养配方有利于慢性肠炎小鼠的恢复,添加CLA或益生菌后效果更为显著。
This research aimed to evaluate the application of conjugated linoleic acid (CLA) as a functional component in formula food for patients with colitis, and probiotics can play an important role in the colon by producing CLA. Therefore, these ingredients were added to nutritional formulas to explore their effects on mice with chronic ulcerative colitis (UC). 50 specific pathogen free (SPF) C57BL/6J mice (male, 8 weeks, 18-20 g) were randomly divided into control group, experimental group including nutrition formula group B, C(including 1% CLA), P(containing 1×109 CFU/mL probiotics). Mice were administered 20-30 g/L dextran sulfate sodium (DSS) in their drinking water for three cycles to induce chronic colitis except the control group. Then the experimental groups were given sterilized water and fed nutrition formula B, C, P ad libitum for 2 weeks. The body weight, colon length, myeloperoxidase (MPO), pathological sections, inflammatory factors, and microbial composition were used as evaluation indicators to assess the effects of different dietary supplements on the recovery of mice with UC. Results showed that all the dietary supplements were beneficial against UC compared with the DSS-induced mice. Among them, diet with CLA or probiotic had better effect on colon length, cytokine expression body weight, MPO, and gut microbial composition, respectively. Nutrition formula diet in this research was beneficial to UC and the effect of diet with CLA or probiotic were better.
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