考察了紫苏籽低聚肽蛋白质含量、低聚肽含量、相对分子质量分布、氨基酸组成以及具有功能活性特征肽段成分。结果显示,蛋白质含量为93.14%,低聚肽含量为86.35%,相对分子质量主要分布在140~1 000 Da,占总比例的82.8%,氨基酸组成较为平衡,精氨酸含量较高,为102.72 mg/g。胱氨酸和蛋氨酸是紫苏籽低聚肽的限制氨基酸。必需氨基酸指数为59.55%,氨基酸组成基本符合FAO/WHO推荐的人体必需氨基酸的构成模式。从紫苏籽低聚肽中鉴定出了2条具有生物活性的二肽结构,分别为甘氨酰精氨酸二肽(glycyl-arginine,GR)和丙氨酰精氨酸二肽(alanlyl-arginine,AR),这2条二肽序列均为国内外首次从紫苏籽低聚肽中鉴定出来。
The contents of protein and oligopeptides, molecular weight distribution, amino acid profile and bioactive peptides of perilla (Perilla frutescens L. Britt.) seed oligopeptides were studied. The results showed that the protein content and the oligopeptides content were 93.14% and 86.35%, respectively. And the relative molecular weight was mainly distributed in the range of 140 – 1 000 Da, which accounted for 82.8% of the total proportion. Additionally, the amino acid composition is relatively balanced, while the arginine content is relatively high, reaching 102.72 mg/g. Cysteine and methionine were the limiting amino acids of perilla seed oligopeptides. The essential amino acid index was 59.55%, and the amino acid composition basically conforms to the pattern of amino acid requirements of adults recommended by FAO/WHO. Moreover, two biologically active dipeptide structures were identified from the perilla seed oligopeptides, namely glycyl-arginine dipeptide (GR) and alanyl-arginine dipeptide (AR). And it was the first time that these two dipeptide sequences were identified from perilla seed oligopeptides.
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