该文以果胶-乳清蛋白混合体系为研究对象,采用Haake RS6000流变仪对钙离子诱导条件下,果胶-乳清蛋白混合体系凝胶形成及流变学特性的影响因素进行研究。结果表明,随着果胶和乳清蛋白添加量的增加,混合体系黏度增强。在一定的乳清蛋白浓度下,随着pH值的增加溶液黏度逐渐减小,当pH=7、乳清蛋白体系质量浓度为40 g/L时,黏度最大(2.304 Pa·s)。果胶-40 g/L乳清蛋白体系能被Ca2+诱导形成凝胶,随着Ca2+浓度增加,黏度值逐渐增大,最高值出现在100 mmol/L处。果胶-40 g/L乳清蛋白混合体系呈现剪切稀化现象。
This paper aimed to understand the gelation mechanism of mixed whey protein-pectin system. The effect of whey protein (WP) concentration, pectin concentration, pH value and Ca2+ on the gel formation and rheological properties of mixed pectin-whey protein system were studied by Haake RS6000 rheometer. The results showed that with the increase of pectin and WP, the viscosity of the mixed system increased. At a certain concentration of WP, the viscosity of the mixture gradually decreased with the increase of pH value. When pH=7 and the concentration of WP system was 40 g/L, the viscosity was the largest (2.304 Pa·s). Moreover, the pectin-40 g/L WP system could be induced by Ca2+ to form a gel. As the concentration of Ca2+ increased, the viscosity value gradually increased, and the highest value appeared at 100 mmol/L. According to the rheological characteristics, the viscosity of mixture decreased gradually with the increase of shear rate, indicating that the mixed whey protein-pectin system was pseudoplastic fluid.
[1] 孙炜,陈璐,刘迪茹,等.热改性聚合乳清蛋白对酸豆奶发酵及贮藏稳定性的影响[J]. 农产品加工(学刊), 2014 (10): 19-22.
[2] WANG Wenbo, BAO Yihong, HENDRICKS G M, et al. Consistency, microstructure and probiotic survivability of goats′ milk yoghurt using polymerized whey protein as a co-thickening agent [J]. International Dairy Journal, 2012, 24 (2): 113-119.
[3] WALSH H, ROSS J, HENDRICKS G, et al. Physico-chemical properties,probiotic survivability, microstructure, and acceptability of a yoghurt-like symbiotic oats-based product using pre-polymerized whey protein as a gelation agent [J]. Journal of Food Science, 2010, 75 (5): 327-337.
[4] 许朵霞,王小亚,尤嘉,等.蛋白质-多糖复合物对β-胡萝卜素乳液的影响[J].食品研究与开发,2012, 33 (4): 9-13.
[5] 王翠娜.聚合乳清蛋白与多糖相互作用对其凝胶特性影响及应用[D]. 长春:吉林大学,2015.
[6] 南海函,郑建仙.乳清蛋白凝胶及其影响因素的机理研究[J]. 广西轻工业,2001 (4): 22-25.
[7] PANG Zhihua, DEETH H, SHARMA R, et al. Rheology, texture and microstructure of gelatin gels with and without milk proteins [J]. Food Hydrocolloids, 2014, 35: 484-493.
[8] 白英,刘乃齐. Enterococcus faecium AS8及其胞外多糖对发酵乳流变学特性的影响[J]. 食品科学, 2019, 40 (10): 70-76.
[9] MCSWEENEY, PAUL L H, FOX P F. Advanced Dairy Chemistry-Volume 1A: Proteins: Basic Aspects[M]. 4th Edition. New York: Springer Science, 2013: 211-273.
[10] SKELTE G A, ANTHONY B M. Reaction kinetics of thermal denaturation of whey proteins in heated reconstituted whole milk [J]. Journal of Agricultural and Food Chemistry, 1996, 44 (2): 422-428.
[11] MIZUHO INAGAKI, KAWAI SHUJI, IJIER X, et al. Effects of heat treatment on conformation and cell growth activity of alpha - lactalbumin and beta-lactoglobulin from market milk [J]. Biomedical Research, 2017, 38 (1): 53-59.
[12] PATRICK F FOX, UNIACKE-LOWE T, PAUL L H MCSWEENEY. Dairy Chemistry and Biochemistry [M]. Switzerland: Springer International Publishing Publishing AG Switzerland, 2015: 164.
[13] 郭本恒.乳品化学[M].北京:中国轻工业出版社, 2001: 83-92.
[14] MIGUEL ANGEL DE LA F, HARJINDER SINGH, YACINE HEMAR. Recent advances in the characterization of heat-induced aggregates and intermediates of whey proteins [J]. Trends in Food Science & Technology, 2002, 13(8): 262-274.
[15] JU Zhiyong, KILARA A. Effects of preheating on properties of aggregates and of cold-set gels of whey protein isolate[J]. Journal of Agricultural and Food Chemistry, 1998, 46 (9): 3 604-3 608.
[16] MARTIN ALBERTO MASUELLI. Viscometric study of pectin. effect of temperature on the hydrodynamic properties [J]. International Journal of Biological Macromolecules, 2010, 48 (2): 286-291.
[17] WANG Cuina, GAO Feng, ZHANG Tiehua, et al. Physiochemical, textural, sensory properties and probiotic survivability of Chinese Laosuan Nai (protein-fortified set yoghurt) using polymerised whey protein as a co-thickening agent [J]. International Journal of Dairy Technology, 2015, 68 (2): 261-269.
[18] VARDHANABHUTI B, FOEGEDING A E, MCGUFFEY K M, et al. Gelation properties of dispersions containing polymerized and native whey protein isolate [J]. Food Hydrocolloids, 2001, 15 (2): 165-175.
[19] MAUD L, ANNE-MARIE H. Fine-stranded and particulate gels of β-lactoglobulin and whey protein at varying ph [J]. Food Hydrocolloids, 1992, 5 (6): 523-539.
[20] 高晓平,黄现青,孙灵霞,等.凝固型酸奶在不同贮藏条件下的品质变化研究[J]. 现代食品科技, 2011, 27 (5): 517-519.
[21] 陈笛,王存芳.不同因素对羊奶热稳定性的影响机理研究现状[J]. 中国乳品工业, 2017, 45 (12): 30-33.
[22] MARCOTTE M, HOSHAHILI R T A, RAMASWAMY H S. Rheological properties of selected hydrocolloids as a function of concentration and temperature[J]. Food Research International, 2001, 34 (8): 695-703.
[23] SINEAD M F, DANIEL M M, EDWIN R M. Segregative interactions between gelatin and polymerised whey protein [J]. Food Hydrocolloids, 2007, 22(3):485-491.
[24] SHAI BARBUT. Effects of calcium level on the structure of pre-heated whey protein isolate gels[J]. LWT-Food Science and Technology, 1995, 28(6):598-603.
[25] 文丽杰.难溶性钙与乳蛋白相互作用及对稳定性的影响[D]. 无锡:江南大学, 2015.
[26] GIRARD, MAUDE, SCHAFFER-LEQUART, CHRISTELLE. Gelation and resistance to shearing of fermented milk: Role of exopolysaccharides [J]. International Dairy Journal, 2007, 17 (6): 666-673.
[27] MENDE S, PETER M, BARTELS K, et al. Addition of purified exopolysaccharide isolates from S. thermophilus to milk and their impact on the rheology of acid gels [J]. Food Hydrocolloids, 2013, 32 (1): 178-185.
[28] 李向东,乔成亚,吕加平,等.弱后酸化发酵剂对长保质期酸奶品质特性影响的研究[J].食品科技, 2008, 33 (12): 99-102.
[29] KONRAAD DULLAERT,JAN MEWIS. Thixotropy: Build-up and breakdown curves during flow[J]. Journal of Rheology, 2005,49 (6): 1 213-1 230.
[30] SEYED M A RAZAVY,HOJJAT KARAZHIYAN. Flow properties and thixotropy of selected hydrocolloids: Experimental and modeling studies [J]. Food Hydrocolloids, 2008, 23 (3): 908-912.