通过生物信息学方法研究豆科植物中木糖异构酶基因家族分类、系统进化关系。从美国国家生物信息中心(National Center for Biotechnology Information,NCBI)网站筛选出34条豆科植物木糖异构酶基因序列,利用WoLF PSORT、MEME、NCBI、ExPASy、PredictProtein以及MEGA等软件分析豆科植物的木糖异构酶基因的亚细胞定位、基序(Motif)、保守区、一级结构、二级结构和进化树。结果表明:从豆科植物中共筛选出34条木糖异构酶基因序列,大部分的序列长度在1 410~1 440 bp;亚细胞定位的主要位置在线粒体、细胞质、内质网;Motif分析显示,34个不同物种中的木糖异构酶可以分为15类Motif;一级结构分析显示,大部分氨基酸的分子质量的范围是52 952.24~54 793.33 Da,等电点的范围为pH 5.52~7.95;二级结构数据显示,无规则卷曲的占比最高;进化树分析显示,34个蛋白序列在进化树上有5个可信度高的分支。研究结论为深入研究豆科植物中木糖异构酶基因的功能奠定了理论基础。
Bioinformatics methods were used to reveal the classification and phylogenetic relationship of xylose isomerase gene family in the Leguminosae. Thirty-four xylose isomerase gene sequences in the Leguminosae were obtained from NCBI website. Their subcellular localizations, motifs, conserved regions, primary structures, secondary structures, and phylogenetic relationships were analyzed with WoLF PSORT, MEME, NCBI, ExPASy, PredictProtein and MEGA, respectively. The results showed that the length of the 34 sequences ranged from 1 410 bp to 1 440 bp, and the main subcellular localizations were mitochondria, cytoplasm and endoplasmic reticulum. Furthermore, motif analysis demonstrated that the 34 sequences could be divided into 15 different motifs. Primary structure analysis demonstrated that the ranges of molecular mass and isoelectric points (pH) were around 52 952.24-54 793.33 Da and 5.52-7.95, respectively. The random coil had the highest proportion through analyzing the secondary structure data. Phylogenetic analysis demonstrated that the xylose isomerase genes could be divided into five groups. This study provides a theory basis for the further research of the xylose isomerase gene family in the Leguminosae.
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