食品与发酵工业 >

2020 , Vol. 46 >Issue 19: 78 - 83

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024164

研究报告

美国豆芋块茎提取物有效成分及其α-葡萄糖苷酶抑制活性

  • 陈镠 ,
  • 卢路路 ,
  • 邓珂 ,
  • 许光治 ,
  • 张有做 ,
  • 倪勤学
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  • (浙江农林大学 农业与食品科学学院,浙江 杭州,311300)
第一作者:硕士研究生(倪勤学副教授为通讯作者,E-mail:niqinxue@zafu.edu.cn)

收稿日期: 2020-04-07

  修回日期: 2020-05-19

  网络出版日期: 2020-11-02

基金资助

浙江省自然科学基金项目(LY19C200016)

收起

Active constituents and α-glucosidase inhibitory activity of Apios americana Medikus tuber extract

  • CHEN Liu ,
  • LU Lulu ,
  • DENG Ke ,
  • XU Guangzhi ,
  • ZHANG Youzuo ,
  • NI Qinxue
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  • (College of Agriculture and Food Science, Zhejiang Agricultural and Forestry University, Hangzhou 311300, China)

Received date: 2020-04-07

  Revised date: 2020-05-19

  Online published: 2020-11-02

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摘要

为探究美国豆芋块茎不同提取物有效成分含量及α-葡萄糖苷酶抑制活性,利用紫外法测定美国豆芋块茎不同极性提取物中总生物碱、总糖、总皂苷、总黄酮和异黄酮含量,并利用不同体外抗氧化体系测定其抗氧化活性和α-葡萄糖苷酶活性抑制能力。结果表明,甲醇提取物中总皂苷、总黄酮和异黄酮含量最高,甲醇提取物抗氧化和α-葡萄糖苷酶抑制活性明显强于其他提取物(P<0.05)。对甲醇提取物进一步分级得石油醚相、乙酸乙酯相、正丁醇相和水相,发现乙酸乙酯相中总皂苷(5.30%)、总黄酮(13.22%)、异黄酮(15.89%)含量均最高,抗氧化活性(DPPH自由基、ABTS阳离子自由基清除能力的IC50值分别为55.573和4.543 μg/mL,FRAP值为50.134 μmol/mg)和α-葡萄糖苷酶抑制能力最强清除能力的(IC50为430.042 μg/mL)。美国豆芋块茎抗氧化活性主要与总糖和总皂苷有关,α-葡萄糖苷酶抑制活性与总糖、总皂苷、总黄酮和异黄酮都密切相关。该研究为美国豆芋块茎在天然抗氧化剂和降血糖方面的应用提供了理论基础,也为合理开发和利用美国豆芋资源提供了科学依据。

关键词： 美国豆芋; 块茎; 有效成分; 抗氧化; α-葡萄糖苷酶抑制活性

本文引用格式

陈镠 , 卢路路 , 邓珂 , 许光治 , 张有做 , 倪勤学 . 美国豆芋块茎提取物有效成分及其α-葡萄糖苷酶抑制活性[J]. 食品与发酵工业, 2020 , 46(19) : 78 -83 . DOI: 10.13995/j.cnki.11-1802/ts.024164

Abstract

To investigate the content of active ingredients and the inhibitory activity of α-glucosidase in different extracts of Apios americana Medikus tubers, the contents of total alkaloids, polysaccharide, total saponins, total flavones and isoflavones in different polar extracts of Apios americana Medikus tubers were determined by UV method; the antioxidant activity and α-glucosidase inhibitory activity were measured. Results indicated that the content of total saponins, total flavonoids and isoflavones in methanol extract was the highest; the antioxidant and α-glucosidase inhibitory activities of methanol extract were significantly stronger than those of other extracts(P<0.05). The methanol extract was further fractionated into petroleum ether phase, ethyl acetate phase, n-butanol phase and aqueous phase. It was found that the content of total saponins (5.30%), total flavones (13.22%) and isoflavones (15.89%) were the highest in the ethyl acetate phase, and the antioxidant activities (DPPH, ABTS radical IC50values were 55.573 μg/mL and 4.543 μg/mL, respectively, and FRAP value was 50.134 μmol/mg) and the inhibitory ability of α-glucosidase was the strongest (IC50was 430.042 μg/mL). The antioxidant activity of Apios americana Medikus tubers is mainly related to polysaccharide and total saponins, and the inhibitory activity of α-glucosidase is closely related to polysaccharide, total saponins, total flavonoids and isoflavones. This study provides a theoretical basis for the application of natural antioxidants and hypoglycemic agents in Apios americana Medikus tubers, and also provides a scientific basis for the rational development and utilization of Apios americana Medikus resources.

Key words： Apios americana Medikus; tuber; active ingredient; antioxidant; α-glucosidase inhibition;

参考文献

[1] CARLISI JACKIE, WOLLARD DENNIS.History,culture,and nutrition of Apios americana[J].Journal of Nutraceuticals, Functional & Medical Foods, 2005, 4(3-4):85-92.
[2] ZHANG Y Z, ZHOU C S, TANG S M, et al.Effect of AATI,a Bowman-Birk type inhibitor from Apios americana,on proliferation of cancer cell lines[J].Food Chemistry, 2011, 128(4):909-915.
[3] MIZUKI T, KAZUHIRO N, ETSUO N.Evaluation of biological activities of a groundnut (Apios americana Medik) extract containing a novel isoflavone[J].Food Chemistry, 2013, 138(1):298-305.
[4] IWAI K, MAITSUEH. Ingestion of Apios americana Medikus tuber suppresses blood pressure and improves plasma lipids in spontaneously hypertensive rats[J].Nutrition Research, 2007, 27(4):218-224.
[5] ZHANG Y Z, KOUZUMA Y, MIUAJI T, et al.Purification,characterization,and cDNA cloning of a Bowman-Birk type trypsin inhibitor from Apios americana Medikus tubers[J].Bioscience Biotechnology & Biochemistry, 2008, 72(1):171-178.
[6] BAMES S, WANG C, KIRK M, et al.HPLC-mass spectrometry of isoflavonoids in soy and the American groundnut,Apios americana[J].Flavonoids in Cell Function, 2002, 505(2):77-88.
[7] 杨芸芸, 楚强, 阎芙洁, 等.美国豆芋的营养成分和生理功能[J].中国粮油学报, 2018, 33(6):141-146.
[8] 戚利潮, 杨芸芸, 郑子桓, 等.美国豆芋不同部位生化特征成分分析[J].浙江农业科学, 2016, 57(4):529-534.
[9] YOUSHIKI Y, OKUBO K.Active oxygen scavenging activity of DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4 H-pyran-4-one) saponin in soybean seed[J]. Bioscience, Biotechnology, and Biochemistry, 1995, 59(8): 1 556-1 557.
[10] KUNISHISA I, MATSUE H J.Ingestion of Apios americana Medikus tuber suppresses blood pressure and improves plasma lipids in spontaneously hypertensive rats[J].Nutrition Research, 2007, 27(4):218-224.
[11] 安家炜,张有做,高前欣, 等.美国豆芋地上部位有效成分及α-葡萄糖苷酶抑制活性研究[J].核农学报, 2014, 28(12):2 275-2 282.
[12] 陈玉婷, 杨云, 王英华. 常用中药化学鉴定[M].北京:化学工业出版社, 2005.
[13] 郝海燕, 沙爱龙. 硬枝碱蓬化学成分的定性分析及生物碱含量的测定[J].食品科技, 2013, 38(1):244-247.
[14] 戴开金, 罗奇志, 罗佳波, 等.葛根芩连方药中黄连总生物碱的含量测定[J].第一军医大学学报, 2004, 24(4):437-438.
[15] 王晶, 王春国, 李冰, 等.苯酚-硫酸法测定慈姑中多糖的含量[J].吉林中医药, 2017, 37(12):1 258-1 260.
[16] YI C, MINGYONG X, XIAOFENGG. Microwave-assisted extraction used for the isolation of total triterpenoidsaponins from Ganodermaatrum[J].Journal of Food Engineering, 2007, 81(1):162-170.
[17] 郑燕菲, 覃小静, 蔡杰慧. 单性木兰总黄酮的含量测定及提取条件优化[J].南方农业, 2019, 13(14):124-126.
[18] 高燕. 不同大豆中异黄酮含量的差异性分析[J].化学工程与装备, 2019(5):9-11.
[19] PRASHANTKUMAR M, RAVINDRA S, PRIYANKA S, et al.Antifungal,anti-aflatoxigenic,and antioxidant efficacy of Jamrosa essential oil for preservation of herbal raw materials[J].International Biodeterioration & Biodegradation, 2012, 74:11-16.
[20] ROBERTA R, NICOLETTA P, et al.Antioxidant activity applying an improved ABTS radical cationdecolorizationassay[J].Free Radical Biology and Medicine, 1999, 26(9-10):1 231-1 237.
[21] 喻巧容, 黄锁义. 不同极性薏苡仁提取物抗氧化活性研究[J].中国医药导报, 2018, 15(19):20-23.
[22] 和焕香, 郭庆梅, 路欣荣. 忍冬果实抗氧化活性研究[J].山东农业科学, 2019, 51(4):61-64.
[23] TANEVA I, PETKOVA N, et al.Characterization of rose hip (Rosa canina L.) fruits extracts and evaluation of their in vitro antioxidant activity[J].Journal of Pharmacognosy and Phytochemistry, 2016, 5(2):35-35.
[24] DONG Q H, LI M, ZHU F.Inhibitory potentil of trilobatin from Lithocarpus polystachyus Rehd against α-glucosidase and α-amylase linked to type 2 diabetes[J].Food Chemistry, 2012, 130(2):261-266.
[25] 程艳刚, 李国艳, 谭金燕,等.分心木总黄酮体外抗氧化活性及对α-葡萄糖苷酶和α-淀粉酶抑制作用研究[J].辽宁中医药大学学报, 2018, 20(8):22-25.
[26] 冯靖,彭效明,李翠清,等.微波-超声辅助联合提取银杏叶中总黄酮的工艺研究[J].食品研究与开发,2019,40(9):68-75.
[27] 王希,刘水平,成琦,等.响应面法优化超声辅助提取竹叶黄酮工艺[J].广州化工,2018,46(12):75-78;105.
[28] 朱盼,谢娟平,向纪明.Box-Behnken响应面法优化秦巴山区粉葛中总异黄酮的提取工艺[J].食品工业科技,2020,41(1):165-170.
[29] 郭婕,胡利宗,李俐俐,等.黑豆豆芽中大豆异黄酮微波提取工艺研究[J].大豆科学,2015,34(3):493-496.
[30] 张红波,孙婕,申娟利,等.响应面法优化大豆异黄酮的提取工艺[J].食品研究与开发,2014,35(24):60-63.
[31] 乔婧,薛晶晶,高建德,等.星点设计-响应面法优化党参总皂苷提取工艺[J].中兽医医药杂志,2019,38(3):36-42.
[32] 张蕾,周萌,倪勤学,等.大越豆芋花醇提物抗氧化及α-葡萄糖苷酶抑制活性研究[J].浙江林业科技,2014,34(4):5-10.
[33] 陈石梅,黄比翼,黄锁义.草果醇提物不同极性部位的体外抗氧化活性研究[J].中国药房,2020,31(8):953-956.
[34] 邱珊莲,林宝妹,张少平,等.嘉宝果嫩叶提取物不同极性部位抗氧化及抑制α-葡萄糖苷酶活性研究[J].热带作物学报,2019,40(7):1 373-1 378.
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