This study aimed to explore the anti-fatigue and antioxidant activity of walnut peptide compound walnut milk by a mouse sports fatigue model, and compare with walnut protein and soybean peptide compounding walnut milk in order to provide a scientific basis for the development of functional walnut milk product. The anti-fatigue and anti-oxidation effects of three kinds of compound walnut milk through various sports experiments, antioxidant and anti-fatigue physiological and biochemical indicators. The results showed that the swimming and hanging scores were ranked as follows: walnut peptide compound walnut milk (WPP) group> soybean peptide compound walnut milk (SP) group> walnut protein compound walnut milk (WEP) group. The WEP group, WPP group and SP group all significantly extended the time of rod climbing and weight-bearing swimming. The WPP group had the best effect, with 183.67 s and 84.75 min, respectively, which were 2.7 times and 3.53 times than the fatigue model (FC) group. All of the three groups could improve the exercise endurance of mice and significantly reduce the levels of lactic acid in serum, skeletal muscle and serum urea nitrogen (P<0.01), among which the highest reductions were 37.81%, 37.2% and 39.40%, respectively. Moreover, the three groups significantly increased the content of liver glycogen and muscle glycogen, reaching a maximum of 7.43 mg/g liver tissue and 1.54 mg/g muscle tissue, respectively. In serum, skeletal muscle and liver, the three groups WEP, WPP and SP showed stronger glutathione peroxidase activity, catalase activity and superoxide dismutase activity than the FC group. Thus, it accelerated the timely removal of malondialdehyde and significantly improved the total antioxidant capacity.
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