采用响应面法优化马齿苋黄酮的提取条件,筛选出最佳水提工艺;通过测定马齿苋黄酮的还原力,1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和羟自由基(·OH)清除能力评价其体外抗氧化活性;通过AAPH诱导建立斑马鱼氧化应激模型,以仔鱼体内活性氧(reactive oxygen species,ROS)产生率、细胞死亡率和脂质过氧化生成率评价马齿苋黄酮对AAPH诱导斑马鱼仔鱼氧化应激的缓解作用。结果表明,马齿苋黄酮的最佳提取工艺为:提取时间55.5 min,提取温度59.27 ℃,料液比1∶19.28(g∶mL),在此条件下黄酮含量达到16.98 mg/g;体外抗氧化活性研究显示马齿苋黄酮具有一定还原力,且对DPPH自由基和·OH均具有较强的清除作用;50~300 μg/mL的马齿苋黄酮预处理可显著降低AAPH诱导后斑马鱼仔鱼ROS产生率,细胞死亡率和脂质过氧化生成率(P<0.05),且其缓解作用呈剂量依赖性。综上,马齿苋黄酮具有较强的体内和体外抗氧化活性,可作为一种天然的抗氧化剂,研究结果将为马齿苋资源的深度开发利用提供数据支撑。
王杰
,
王瑞芳
,
王园
,
任雪荣
,
陈秋燕
,
齐景伟
,
王浩然
,
杨帆
,
赵旭阳
,
郭文浩
,
安晓萍
. 响应面优化马齿苋黄酮水提工艺及其抗氧化活性评价[J]. 食品与发酵工业, 2020
, 46(19)
: 197
-204
.
DOI: 10.13995/j.cnki.11-1802/ts.024263
The present study aimed to explore the optimum water extraction conditions of flavonoids from Portulaca oleracea L. and evaluate its antioxidant activity in vitro and in vivo. Extraction time, temperature and material-water ratio were enrolled in this experiment, and total flavonoids was used to evaluate the extraction process. The response surface methodology was used to optimize the extraction process. Moreover, the antioxidant activity of flavonoids in vitro was evaluated by measuring the reducing power, the scavenging ability of DPPH and hydroxyl radicals. And the in vivo activity was assessed by establishing oxidative stress model of zebrafish induced by AAPH. The results showed that the optimized water extraction process was as follows: extraction was performed at 59.27 ℃ for 55.5 min and the ratio of material to water was 1∶19.28(g∶mL). Under the optimal conditions, the content of flavonoids from P. oleracea L. was 16.98 mg/g. Moreover, the antioxidant results showed that flavonoids from P. oleracea L. had a certain reducing power and strong scavenging effect on DPPH and hydroxyl radicals. Different concentrations of purslane flavonoids (50, 100, 200, 300 μg/mL) could significantly reduce the ROS production rate, cell death rate and lipid peroxidation production rate of zebrafish larval induced by AAPH. And this alleviatory effect of flavonoids on oxidative stress of zebrafish was dose-dependent. Therefore, the analysis results obtained by response surface method are reliable, efficient and simple, and can be used for extraction of flavonoids from P. oleracea L. The flavonoids from P. oleracea L. exhibit obvious antioxidant activity in vitro and in vivo and could be used as a natural antioxidant. This is the first time to report in vivo antioxidant effect of flavonoids from P. oleracea L. through zebrafish model. It provides a basis for deep exploitation and utilization of flavonoids from P. oleracea L.
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