分析与检测

不同品种海棠叶茶游离氨基酸组成分析与评价

  • 云金虎 ,
  • 江皓 ,
  • 韩文学 ,
  • 卞健 ,
  • 孙艳艳 ,
  • 张往祥
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  • 1(南京林业大学 林学院,南方现代林业协同创新中心,江苏 南京,210037);
    2(扬州小苹果园艺有限公司,江苏 扬州,225200)
第一作者:硕士研究生(张往祥教授为通讯作者,E-mail:malus2011@163.com)

收稿日期: 2020-04-24

  修回日期: 2020-05-31

  网络出版日期: 2020-11-02

基金资助

现代农业重点项目(BE2019389); 林业科学技术推广项目【2019】17号

Analysis and evaluation of free amino acid in different cultivars of crabapple leaf tea

  • YUN Jinhu ,
  • JIANG Hao ,
  • HAN Wenxue ,
  • BIAN Jian ,
  • SUN Yanyan ,
  • ZHANG Wangxiang
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  • 1(College of Forestry, Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University, Nanjing 210037, China);
    2(Yangzhou Crabapple Horticulture Limited Company, Yangzhou 225200, China)

Received date: 2020-04-24

  Revised date: 2020-05-31

  Online published: 2020-11-02

摘要

为了探究不同海棠品种叶片的茶用开发价值,以15个海棠品种嫩芽制成的海棠叶茶为材料,利用全自动氨基酸分析仪对海棠叶茶游离氨基酸的含量和种类进行检测。通过味道活性值(taste active value,TAV)、氨基酸评分(amino acid score,AAS)和必需氨基酸指数(essential amino acid index,EAAI)对氨基酸进行呈味分析和营养评价。结果表明:15个海棠品种叶茶均含有17种氨基酸,游离氨基酸的总量为655.12~3059.69 mg/100 g,必需氨基酸占氨基酸总量的8.49%~37.53%,药用氨基酸含量占氨基酸总量的25.87%~58.14%;基于氨基酸评分,海棠叶茶的第一限制性氨基酸为蛋氨酸和半胱氨酸,必需氨基酸指数最平衡的品种为‘黛玉'、‘完美紫'和‘丰盛'通过味道活性值的比较,谷氨酸和丝氨酸对呈味贡献最大;利用主成分分析,从氨基酸角度较好地反映出不同海棠品种之间的差异性。该研究为海棠叶茶营养价值及其叶片资源的有效利用提供了参考和依据。

本文引用格式

云金虎 , 江皓 , 韩文学 , 卞健 , 孙艳艳 , 张往祥 . 不同品种海棠叶茶游离氨基酸组成分析与评价[J]. 食品与发酵工业, 2020 , 46(19) : 237 -243 . DOI: 10.13995/j.cnki.11-1802/ts.024288

Abstract

To explore the value of crabapple leave, the free amino acids in crabapple leaf tea made from the buds of 15 crabapple cultivars, were detected by automatic amino acid analyzer. The taste activity value, amino acid score and essential amino acid index were used for the evaluation. The results showed that there were 17 amino acids that could be detected in the leaves of 15 crabapple cultivars, including the total amount of free amino acids 655.12-3 059.69 mg/100 g. Moreover, the essential amino acids and the medicinal amino acids account for 8.49%-37.53% and 25.87%-58.14% of the total amino acids, respectively. Based on the amino acid score, the top two limiting amino acids of crabapple leaf tea were Met and Cys, and the most balanced essential amino acid index were M. ‘Black Jade', M. ‘Purple prince' and M. “Profussion”. Glu and Ser contribute most to the flavor compared with taste active value. The principal component analysis revealed the differences of different crabapple cultivars. This study provides reference and basis for the nutritional value of crabapple leaf tea and its effective utilization of leaf resources.

参考文献

[1] 李育农. 苹果属植物种质资源研究[M]. 北京: 中国农业出版社, 2001.
[2] 周婷, 沈星诚, 周道建, 等. 海棠品种分类研究进展[J]. 园艺学报, 2018,45(2):380-396.
[3] 姜文龙,范俊俊,张丹丹,等.观赏海棠不同叶位色彩特征及特异种质挖掘[J].园艺学报,2017,44(6):1 135-1 144.
[4] 李珮, 申洁, 毕武, 等. 苹果属植物叶片的代谢组学分析及5种成分的含量测定研究[J]. 中草药, 2018, 49(22): 5 378-5 387.
[5] LIU Bihui, ZHANG Chengfeng, ZHANG jing, et al. Wu shan shen cha(Malus asiatica Nakai. leaves)-derived flavonoids alleviate alcohol-induced gastric injury in mice via an anti-oxidative mechanism[J]. Biomolecules,2019,9(5):169.
[6] 毛世红. 基于风味组学的工夫红茶品质分析与控制研究[D]. 重庆: 西南大学, 2018.
[7] TSENG Y H, LEE Y L, LI R C, et al.Non-volatile flavour components of Ganoderma tsugae[J]. Food Chemistry, 2005, 90(3):409-415.
[8] 闫征, 郝利民, 张黎明, 等. 3种不同产地灵芝子实体主要化学成分比较[J]. 食品科学, 2019,40(6):240-246.
[9] HORANNI R, ENGELHARDT U H.Determination of amino acids in white, green, black, oolong, pu-erh teas and tea products[J]. Journal of Food Composition and Analysis, 2013, 31(1):94-100.
[10] YıLMAZ C, ÖZDEMIR F,GÖKMEN V. Investigation of free amino acids, bioactive and neuroactive compounds in different types of tea and effect of black tea processing[J]. LWT, 2020,117.
[11] GB/T 30987—2014 植物中游离氨基酸的测定[S]B/T 30987—2014 植物中游离氨基酸的测定[S]. 北京:中国标准出版社, 2014.
[12] ZHOU Mengyi, HUA Tongtong, MA Xiaofang, et al.Protein content and amino acids profile in 10 cultivars of ginkgo (Ginkgo biloba L.) nut from China[J]. Royal Society Open Science, 2019,6(3): 181 571.
[13] 王馨雨, 王蓉蓉, 杨绿竹, 等. 不同品种及内外百合鳞片游离氨基酸组成的主成分分析及聚类分析[J]. 食品科学,2020,41(12):211-220.
[14] 张晓煜, 刘静, 袁海燕, 等. 不同地域环境对枸杞蛋白质和药用氨基酸含量的影响[J]. 干旱地区农业研究, 2004,22(3): 100-104.
[15] 侯娜, 赵莉莉, 魏安智, 等. 不同种质花椒氨基酸组成及营养价值评价[J].食品科学, 2017,38(18):113-118.
[16] 秦颖超, 周加义, 朱敏, 等. 谷氨酸吸收转运及对肠道发育影响的研究进展[J]. 动物营养学报, 2019,31(2):544-552.
[17] BROSNAN J T, BROSNAN M E.Glutamate: a truly functional amino acid[J]. Amino Acids, 2013, 45(3):413-418.
[18] 杨小芳, 王文建, 刘建福, 等. 枇杷花茶氨基酸组成分析及营养价值评价[J]. 安徽农业大学学报, 2018,45(2):225-228.
[19] 刘建福, 高俊杰, 田奥磊, 等. 武夷岩茶氨基酸组分及含量分析[J]. 热带作物学报, 2017,38(2):283-287.
[20] 陈玉芹, 尹红星, 王应清, 等. 辣木茶的氨基酸分析及营养价值评定[J]. 南方农业学报, 2017,48(7):1 280-1 285.
[21] GONZALEZ J A, KONISHI Yotaro, BRUNO M, et al.Interrelationships among seed yield, total protein and amino acid composition of ten quinoa (Chenopodium quinoa) cultivars from two different agroecological regions[J]. Journal of the Science of Food and Agriculture, 2012, 92(6): 1 222-1 229.
[22] CHAVEROUX C, LAMBERTL-ANGLAIS S, CHERASSE Y, et al.Molecular Nechanisms involved in the adaptation to aminoacid limitation in mammals[J]. Biochimie, 2010, 92(7):736-745.
[23] LIOE H N, APRIYANTONO A, TAKARA K, et al.Umami taste enhancement of MSG/NaCl mixtures by subthreshold L-α-aromatic amino acids[J]. Journal of Food Science, 2005,70(7): s401-s405.
[24] 刘伟, 张群, 李志坚, 等. 不同品种黄花菜游离氨基酸组成的主成分分析及聚类分析[J]. 食品科学, 2019, 40(10):243-250.
[25] 黄翠香. 海棠优系制茶的品质研究[J].中国农学通报,2019,35(36):61-66.
[26] 王春叶, 童华荣. 滋味稀释分析及其在食品滋味活性成分分析中的应用[J]. 食品与发酵工业, 2007, 33(12):117-121.
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