通过优化获得适合双歧杆菌工业化生产的,成本低廉的工艺条件。通过单因素试验,从番茄汁、低聚果糖、废糖蜜、马铃薯淀粉、玉米浆、豆饼粉6个因素中选出较优因素,并通过L9(3)4正交试验确定双歧杆菌发酵培养基的适宜配方及适宜发酵条件。结果表明:适宜的发酵培养基组成为番茄汁80ml/L、低聚果糖15g/L、废糖蜜20ml/L、玉米浆110mL/L、0.1%刃天青1mL/L、吐温-801mL/L、盐溶液40mL/L及L-半胱氨酸盐酸0.5g/L;适宜的培养条件为温度40℃、起始pH6.8、接种量3%,发酵时间32h。优化得到的培养基成本较低,发酵条件简单易控,适合双歧杆菌工业化生产。

In this study,the culture medium element and fermentation conditions of Bifidobacterium bifidum were optimized to determine the technical conditions that are beneficial for industrial batch production at low cost.Four out of six factors,namely tomato juice,fructo-oligosaccharide,waste molasses,potato starch,corn steep liquor,bean cake powder,were selected through single factor experiments.By conducting orthogonal experiments with the orthogonal table L9(3)4,we determined the optimum medium formula and the favorite fermentation conditions.The medium formula were:tomato juice 80 ml/L,fructo-oligosaccharide15 g/L,waste molasses 20 ml/L,corn steep liquor 110 ml/L,0.1%resazurin1 ml/L,tween-80 1 ml/L,salt solution 40 ml/L,L-cysteine hydrochloride0.5 g/L;the favorable fermentation conditions were temperature 40 ℃,initial pH 6.8,inoculation amount 3%,and culture hour 32 h.The low production cost and the easy-to-control fermentation conditions make the optimum medium element suitable for industrial batch production of Bifidobacterium bifidum.