以滤纸作为唯一碳源,成功从人体肠道微生物中筛选分离出1株产纤维素酶的菌株H1。分离富集后的菌株用纤维素刚果红培养基进行筛选鉴定、革兰色染色、对菌株进行分类并对该菌种进行CMC酶活测定与分析、分子鉴定。H1在纤维素刚果红平板上能呈现阳性反应;镜检发现,H1菌株为短杆状,革兰色阴性菌;该菌株在13h酶活最大,为0.8U左右;分子鉴定结果为假单孢菌属。

Cellulase-secreting microorganisms include fungi and a few cellulose-decomposing bacteria.In this study,human rectal microbiota were screened by using filter paper as the only carbon source,and one cellulase-secreting bacteria,H1,was successfully separated.Its cellulase-secreting activity was identified using cellulose congo-red medium,morphology identification undertaken by gram staining,and classification accomplished via molecular identification.The cellulase activity of H1 was also analyzed.The results show it is positive in the cellulose congo-red medium,short and rod-shaped and gram-negative.Cellulase activity analysis indicated that its highest cellulase activity is about 0.8 U occurred at 13 hours.Molecular identification characterized the strain as Pseudomonas specie.