研究建立了食品中鱼源性成分的普通PCR检测方法,该方法特异、灵敏。应用鱼源性引物对35种鱼类、24种非鱼类动物和10种植物样品进行PCR检测,只在35种鱼类中出现224bp特异扩增条带,在其他的非鱼类动植物DNA中未出现扩增条带,实验表明,该PCR检测方法具有特异性。该检测方法的检测限为0.1ngDNA和0.1%(W/W)。运用建立的方法对市场上的80个不同类别的食品样品进行检测,其中有13个样品错误标识了鱼源性成分。该检测方法能够用于食品中鱼源性成分的鉴别。
A specific and sensitive PCR method of fish derived material in food was developed.Primers fish1 and fish2 amplified a 224bp fragment from 35 fish samples,whereas no amplification was obtained from 24 other animal samples and 10 plant samples.The detection limit of the assay was 0.1ng DNA and 0.1%(W/W).80 food samples were analyzed by PCR,fish derived material were labeled incorrectly in 13 samples.The detection method can be used to identify fish derived material in food.
