研究了嗜热枯草芽孢杆菌TT68的发酵条件的优化。经碳源、氮源、碳氮比、氨基酸和添加维生素5个单因素发酵条件的优化,得到枯草芽孢杆菌TT68发酵产β-甘露聚糖酶的最适摇瓶发酵培养条件:4g/L魔芋精粉为碳源,0.7g/L豆粕粉为氮源,碳氮比为6∶1,2g/LL-缬氨酸和0.75mg/L盐酸吡哆辛。在上述条件下培养6d,胞外β-甘露聚糖酶活性最高,可达7500U/mL。采用5L发酵罐进行放大培养,48h酶活即达到最高,可达9870U/mL。该嗜热枯草芽孢杆菌能利用廉价的碳源和氮源高产甘露聚糖酶,且发酵周期短,在工业化生产甘露聚糖酶中具有很大的应用潜力。

The thermophilic Bacillus subtilis TT68 was optimized for β-mannanase production in the present report. The fermentation conditions,including carbon source,nitrogen source,ratio of carbon source and nitrogen source,amino acids and vitamins,were optimized in order to improve the production of β-mannanase. The optimized medium is composed of 4 g/L konjac powder as carbon source,0.7 g/L soybean meal powder,2 g/L L-valine,and 0.75 mg/L Pyridoxine hydrochloride (VB6). The highest enzyme activity of 7500 U/mL was obtained after incubation for 6 days in the optimized medium. When the strain was incubated using a 5 L fermentor for 2 days,the activity was up to 9870 U/mL. Bacillus subtilis TT 68 could produce a high yield of β-mannanase utilizing the inexpensive carbon source and nitrogen source in a short period. Thus,the strain displays a great potential for the industrial production of β-mannanase.