从广州花都芙蓉彰分离纯化得到1株产漆酶的野生灵芝菌株,形态学与分子鉴定表明该菌株为韦伯灵芝。对该菌株液态发酵产漆酶的营养条件进行了优化,并就粗酶液对靛蓝染料的脱色能力进行了探讨。结果表明,发酵产漆酶的最佳碳源为35.0g/L麸皮和10.0g/L葡萄糖;最佳氮源为2.5g/L酵母膏;添加0.5mmol/L的Cu2+对漆酶的合成有一定促进作用,在优化条件下培养6d,漆酶活力高达10943.8U/L,是优化前的68.3倍。采用漆酶粗酶液可直接对靛蓝染料(100mg/L)进行有效脱色,在pH3.0、50℃条件下反应120min,脱色率可达92.6%。

A wild Ganoderma strain was isolated from Furongzhang in Huadu,Guangzhou. It was identified as Ganoderma weberianum by its morphological and molecular characteristics. The culture conditions for laccase production by Ganoderma weberianum was optimized in liquid fermentation and the discoloration ability of crude laccase for indigo dye was investigated. The results showed that wheat bran and glucose were the best carbon source for laccase production by Ganoderma weberianum and their optimal concentration were 35 g/L and 10 g/L,respectively. Yeast extract was the best nitrogen source for laccase production and its optimal concentration was 2.5 g/L. Cu2+ could enhance laccase production. Laccase activity in the ferment medium under the optimal culture conditions was 10 943.8 U/L after 6-day incubation,which was 68.3 fold of that of the original medium. Under the condition of pH3.0 and 50 ℃,indigo dye could be decolorized effectively by crude laccase,the decolorization rate reached 92.6% after 120-min reaction.